HSE-HA Stimulator CS for Isolated Cells
Electrical square wave stimulator especially designed for electrical field stimulation of isolated cells, e.g. myocytes. Can be used for other stimulation applications where high currents are necessary.
- Easy to use
- Constant voltage mono-phasic, biphasic or alternating stimulation (>1 A)
- High output impulse current
- Backup memory for timing setting
- Specially designed for isolated cell stimulation
The stimulator CS Type 223 is an electronic square wave stimulator especially designed for the electrical stimulation of isolated cells, e.g. myocytes. Stimulating isolated cells needs a high impulse current to trigger a maximum of cells independent of the size, shape specially in cell culture medium having a low resistance. This is of importance in experiments where the threshold voltage of cell reaction has to be determined. To reduce electrochemistry effects such as polarization of the electrodes and double layer of gas bubbles biphasic stimulation with perfect symmetry is requested. To be able to activate an action potential by depolarizing the membrane and to avoid further influence of the stimuli on the cell reaction, short and powerful impulses are required.
- Easy to use
- Constant voltage mono-phasic, biphasic or alternating stimulation (>1 A)
- High output impulse current
- Backup memory for timing setting
- Specially designed for isolated cell stimulation
Stimulation Mode and Timing are displayed on a LCD display and can easily be set in a step through mode using the ‘Menu’ button and the adjust knob. The last setting of the timing is stored in a backup memory and recalled automatically after switching on the instrument. The Stimulus Output Voltage is indicated on the built-in digital voltmeter and can be adjusted with the AMPLITUDE knob on two potentiometers with adjustment in the range of 0 to 45 V. The output is floating; not connected to earth (ungrounded). The output has a very low impedance (less than 1 Ohm). To avoid overheating of the output circuit a dynamic current limiter is built in. It limits the maximum output current relative to the stimulation width.
| Specifications | 73-2667 | 73-0485 |
|---|---|---|
| Delay Time | (leading the stimuli): 2 to 50 msec (in 7 steps) 2, 5, 10, 20, 30, 40, 50 msec | (leading the stimuli): 2 to 50 msec (in 7 steps) 2, 5, 10, 20, 30, 40, 50 msec |
| Dimension (H x W x D) English | 4.3 x 11.8 x 9.8 in | 4.3 x 11.8 x 9.8 in |
| Dimensions (H x W x D) Metric | 100 x 300 x 250 mm | 100 x 300 x 250 mm |
| Indicators - 1 | 1. LCD module (4 lines of 16 characters each) to indicate stimulus parameter settings (except output voltage)
2. Digital voltmeter (DVM) to indicate output voltage setting range selectable: 0 - 50.0 V | 1. LCD module (4 lines of 16 characters each) to indicate stimulus parameter settings (except output voltage)
2. Digital voltmeter (DVM) to indicate output voltage setting range selectable: 0 - 50.0 V |
| Indicators - 2 | 3. LED for stimulus pulse
4. Beep (with each stimulus pulse), can be switched off | 3. LED for stimulus pulse
4. Beep (with each stimulus pulse), can be switched off |
| Stimulation Mode 1 | SINGLE/STRT single stimulus triggered by START switch or by remote contact | SINGLE/STRT single stimulus triggered by START switch or by remote contact |
| Stimulation Mode 2 | REPEAT/STRT repeating stimulus triggered by START switch or by remote contact | REPEAT/STRT repeating stimulus triggered by START switch or by remote contact |
| Stimulation Mode 3 | TRIGGER pulse (TTL, negative-going edge) starts delay circuit; START switch must be in OFF position | TRIGGER pulse (TTL, negative-going edge) starts delay circuit; START switch must be in OFF position |
| Stimulus Output | 1. Output voltage 0 to 50 V; Pulse Current up to 1 A at max. pulse width (10 msec)
2. Monophasic, Biphasic, Alternated 3. Output circuit floating, not connected to earth (ungrounded) | 1. Output voltage 0 to 50 V; Pulse Current up to 1 A at max. pulse width (10 msec)
2. Monophasic, Biphasic, Alternated 3. Output circuit floating, not connected to earth (ungrounded) |
| Timing: Stimulation Frequency | 0.05 to 100 Hz (in 29 steps) 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 Hz | 0.05 to 100 Hz (in 29 steps) 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 Hz |
| Timing: Stimulus Width | 10 µsec to 10 msec (in 28 steps) 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 µsec | 10 µsec to 10 msec (in 28 steps) 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 µsec |
| Trigger Input | BNC socket, input signal starts time delay, TTL level, negative active edge | BNC socket, input signal starts time delay, TTL level, negative active edge |
| Trigger Output | BNC socket, signal: delay time, open collector output (2 kΩ to +5V) | BNC socket, signal: delay time, open collector output (2 kΩ to +5V) |
| Weight (English) | 7.7 lbs | 7.7 lbs |
| Weight (Metric) | 3.5 kg | 3.5 kg |
