Excised Patch Chamber with Two Slotted Open Baths (RC-28)

Excised Patch Chamber with Two Slotted Open Baths (RC-28)

The dual slotted open bath construction of the excise patch chamber permits exchange of solution surrounding a membrane patch without contamination of the associated cell population.

  • Designed for excised patch studies
  • Open chamber design
  • Dual slotted baths in two sizes
  • Baths separated by a controllable dam

Item# Description U.S. List Price Quantity
64-0243 Excised Patch RC-28 Recording Chamber
64-0282 Series 20 Plain Platform, Model P-6
64-0289 Series 20 Heater Platform, Model PH-6
64-1527 PM-6 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-0709 CS-24/50 Coverslips for use with RC-27, RC-27L, RC-27N, RC-27NE, RC-28, and RC-29, 0.15 mm (0.006 in)24 x 50 mm, pkg of 65
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1431 Straight Suction Tube, Series 20 Classic Design for use with RC-24, RC-24E, and RC-28 chambers
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The RC-28 is a special chamber containing two bath compartments separated by an adjustable dam. The dam functions both as a gate and barrier between the baths. The composition and flow rate of each bath can be independently controlled, and each bath has a separate stainless steel suction tube for perfusion.

The dual slotted, open bath design allows for the exchange of solution surrounding an isolated membrane patch without contaminating or modifying the rest of the population. Raising the solution level in the dam area joins the two baths, while lowering the solution level isolates the two baths. After patching onto a cell in the large bath compartment, the dam is flooded, enabling secure transfer of the electrode attached cell to the smaller compartment. The dam is then drained isolating the two baths.

For the larger chamber, the bath area is 21 x 9.5 mm and the working volume is 243 ul per 1.0 mm of solution height. For the smaller chamber, the bath area is 28 x 3.8 mm and the volume is 111 ul.

A platform (PM-6, PH-6, or P-6) and stage adapter are required to complete the assembly for mounting onto a microscope. The chamber bottom is formed by a 24 x 50 mm coverslip.

A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

The RC-28 is supplied with a pack of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, 50/pkg) and 10 ft of PE-160 polyethylene tubing.

Material Polycarbonate
Dimensions, small bath d
(L x W x H)
20.0 x 3.8 x 4.5 mm
Dimensions, large bath d
(L x W x H)
28.0 x 9.5 x 4.5 mm
Volume, small bath, by depth 111 µl
Volume, large bath, by depth 243 µl
Specimen area, small bath (L x W) 28 x 3.8 mm
Specimen area, large bath (L x W) 21 x 9.5 mm
Bottom coverglass 24 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

RC-28 Drawing

  1. Barium, TEA and sodium sensitive potassium channels are present in the human placental syncytiotrophoblast apical membrane
    Díaz P, Vallejos C, Guerrero I, Riquelme G.
    Placenta. 2008 Oct;29(10):883-91
  2. The maxi-chloride channel in human syncytiotrophoblast: a pathway for taurine efflux in placental volume regulation
    Vallejos C, Riquelme G
    Placenta. 2007 Nov-Dec;28(11-12):1182-91
  3. Correlative Studies of Gating in Cx46 and Cx50 Hemichannels and Gap Junction Channels
    Miduturu Srinivas, Jack Kronengold, Feliksas F. Bukauskas,y Thaddeus A. Bargiello, and Vytas K. Verselisy
    Biophysical Journal Volume 88 March 2005 1725–1739
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-28 (080724).pdfRC-28 Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)