ElectroPrep Electrodialysis System

ElectroPrep Electrodialysis System

The QuikPrep ElectroPrep Electrodialysis System is an extremely versatile patented sample preparation technology that is capable of separating samples by both size and charge. It provides faster dialysis times due to movement of charged molecules in an electric field during dialysis, thus combining electrophoresis with dialysis. With a run-time of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at the very low currents (5 to 10 mA) used with this system.

It is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules. Membranes of different MWCO (molecular weight cut off), from 100 to 300,000 Daltons, can be used for selective buffer exchange, dialysis, filtration, concentration, fractionation and elution.

Item# Description U.S. List Price Quantity
74-1196 ElectroPrep Tank, with lid, gasket, 4 mm red and black connector cables
74-1197 ElectroPrep Connector Cables, 4 mm, red and black (1 each)
74-1103 Power Supply for Electroprep, 200 V, 100 mAmp
7411-502D Dialysis Chamber for ElectroPrep, 50 µl, Qty. of 2
7411-1002D Dialysis Chamber for ElectroPrep, 100 µl, Qty. of 2
7411-2502D Dialysis Chamber for ElectroPrep, 250 µl, Qty. of 2
7411-5002D Dialysis Chamber for ElectroPrep, 500 µl, Qty. of 2
7411-10002D Dialysis Chamber for ElectroPrep, 1000 µl, Qty. of 2
7411-15002D Dialysis Chamber for ElectroPrep, 1500 µl, Qty. of 2
7411-502L Link Chamber for ElectroPrep, 50 µl, Qty. of 2
7411-1002L Link Chamber for ElectroPrep, 100 µl, Qty. of 2
7411-2502L Link Chamber for ElectroPrep, 250 µl, Qty. of 2
7411-5002L Link Chamber for ElectroPrep, 500 µl, Qty. of 2
7411-10002L Link Chamber for ElectroPrep, 1000 µl, Qty. of 2
7411-15002L Link Chamber for ElectroPrep, 1500 µl, Qty. of 2
74-1194 Union (Connector), (1) 600 µl and (1) 3500 µl, to join ElectroPrep Dialysis Chambers
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The QuikPrep ElectroPrep Electrodialysis System is an extremely versatile patented sample preparation technology that is capable of separating samples by both size and charge. It provides faster dialysis times due to movement of charged molecules in an electric field during dialysis, thus combining electrophoresis with dialysis. With a run-time of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at the very low currents (5 to 10 mA) used with this system.

It is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules. Membranes of different MWCO (molecular weight cut off), from 100 to 300,000 Daltons, can be used for selective buffer exchange, dialysis, filtration, concentration, fractionation and elution.

 The ElectroPrep Tank (74-1196) is supplied with a tank, lid and connectors, and a replacement gasket. Power supply adapters must be purchased separately.

Features

  • Faster dialysis times due to movement of charged molecules in the electric field
  • Reusable
  • Available for most sample sizes, large or small
  • Membranes available with MWCO’s to suit almost any application
  • Easy to use
  • Leak proof
  • Autoclaveable
  • Low protein binding
  • High sample recovery
  • Made of totally insert PTFE

Applications

  • Electro-elution from gels and solutions
  • Electro-dialysis (with an average buffer exchange time of 5 to 10 minutes)
  • On-line electrodialysis
  • Electroconcentration
  • Selective electrofiltration
  • Size fractionation
  • Primer removal
  • Salt removal
  • Detergent removal
  • Dye terminator removal


Assembly and Use

A functional ElectroPrep System consists of the ElectroPrep Tank, power supply, and one or more Dialyzer Units.

The ElectroPrep System uses at least one Dialyzer Unit to perform a sample electrodialysis. A basic Dialyzer Unit is comprised of a dialyzer chamber, dialysis membranes at one or both ends of the chamber and two end caps.

Dialyzer Units can be configured in a number of ways to perform different applications using a combination of components:

  • Dialysis Chamber: the major receptacles for either samples or dialyzed materials. Includes one main chamber with two open ports and two matching end caps. The sample chambers are made of PTFE, a completely inert material especially suited for high sample recovery.
  • Union: joins two dialysis chambers together
    • Without membranes to make a larger volume chamber
    • With dialysis membranes of appropriate MWCOs for serial dialysis
    • Two end caps are used, one at the end of each chamber
  • Link Chamber: can be joined directly to a dialysis chamber or to another link chamber to concentrate dialyzed sample or for size fractionation using membranes of different MWCOs. Includes one open end cap.
  • Dialysis membranes are added at one or both ends or between Dialysis Chamber and/or Link Chambers and unions. Membranes with molecular weight cut off points (MWCOs), ranging from 500 to 300,000 daltons, may be used in combination with different Dialysis and Link Chambers for selective elution, filtration, dialysis, fractionation and concentration of complex samples.

 

electroprep components


How to Select Your Chamber and Membrane Configuration

  1. Decide upon your application, e.g., electrodialysis, electroelution, electrofiltration, electroconcentration, electroseparation.
  2. Select a Dialysis Chamber able to hold the desired sample volume (50 to 1500 µl.) Note that a Dialysis Chamber can be joined to a Union to increase sample volume (up to 600 or 3500 µl).
  3. Choose Dialysis Membranes of suitable size, type and MWCO depending on the application being done and the molecular weight of the biological molecule of interest. Take into account the membrane’s suitability for use in aqueous or organic solvents.
    - For organic solvents, use either regenerated cellulose or polycarbonate.
    - For aqueous solutions, use cellulose acetate
  4. Assemble Dialysis Unit
    - With a membrane and open-ended Caps for desalting or buffer exchange (Configuration #1)
    - With a membrane, Union and a smaller volume Chamber for electroconcentration (Configuration #2)
    - With a membrane, Union and equal volume Chamber for electroseparation and electroelution (Configuration #2)
    - With a membrane and Link Chambers for electrofiltration (Configuration #3)
    - With membranes of different MWCO and multiple Link Chambers for electrofractionation (Configuration #4)

Example Configurations

 

electroprep configurations

Specifications