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EasyCell System for Cell Isolation

Price on Request
SKU
GRP-Easycell-All

Used to isolate primary cells from hearts or other organs of species in the size range of mouse to guinea pigs. 

To ensure that your system is properly configured as a complete setup that meets your experimental needs, please email us at sales@harvardapparatus.com or call us at 800-597-0580.  In Europe, please call +49 7665 92000 or email sales@hugo-sachs.de.

Easily compare our Cell Isolation Systems. Download our Cell Isolation Systems Guide.

Grouped product items
Item No. Description Qty
73-5153 EasyCell Basic Constant Flow Cardiomyocyte Isolation System for Small Rodents (115 V) Please login to add to Quote
73-3756 Easy Setup for Cell Extraction: Includes Base Stand with Clamps, Heating Coil, Bubble Trap, 2x Gas Needle Valves and Protease Reservoir Please login to add to Quote

This perfusion system has been specifically created to isolate primary cells from hearts of species in the size range of mouse to guinea pigs. The system can also be configured for either in situ or ex vivo organ perfusion with the addition of an operating table or moist chamber and appropriate cannulae.

Cardiomyocyte Isolation

Primary Cell Isolation from Other Organs


 

 

Cardiomyocyte Isolation

Features & Benefits

  • Simple constant flow perfusion system
  • Easy to set up, operate and maintain
  • Temperature control—water-jacketed heat exchanger and heart chamber for maintenance of physiological temperature
  • Pulsation Dampening—air vessel/compliance chamber dampens perfusion pressure pulsation generated by the peristaltic pump. Serves as primary bubble trap to prevent air emboli from causing regional ischemia, increasing potential cell yield
  • Wetted parts can be disinfected with 70% ethanol or autoclaved for sterilization

Applications

  • Cardiomyocyte isolation from small rodent hearts
  • Can be adapted for other small rodent organs in situ or ex vivo
  • Basic research or student lab use

Included Items

230 V EasyCell System (73-4431) 115 V EasyCell System (73-5153)
73-3756 Easy Setup for Cell Extraction by Organ Disintegration, consisting of Base Stand with Clamps, Bubble Trap/Windkessel, Heating Coil, Protease Reservoir 73-3756 Easy Setup for Cell Extraction by Organ Disintegration consisting of Base Stand with Clamps, Bubble Trap/Windkessel, Heating Coil, Protease Reservoir
73-3761 Heart Chamber (ID 40 mm x 60 mm deep, Length 110 mm, OD 60 mm) with Mounting Clamp 73-3761 Heart Chamber (ID 40 mm x 60 mm deep, Length 110 mm, OD 60 mm) with Mounting Clamp
73-3438 Jacketed Reservoir for Buffer Solution with Frit, 1 L 73-3438 Jacketed Reservoir for Buffer Solution with Frit, 1 L
73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valve 73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valve
 75-1004 Reglo Peristaltic (Roller) Pump 70-7000 Peristaltic Pump P70
73-4544 TC120 Thermocirculator, with 5 L stainless steel bath and lid, 220 V 73-4545 TC120 Thermocirculator, with 5 L stainless steel bath and lid, 120 V
73-1837 3-Stop Tygon® E-Lab Tubing, 2.29 mm ID, 12/pack, Purple/White 72-0668 3-Stop PVC Tubing, 2.05 mm ID, Purple/Purple, pkg. of 6
73-0126 3-Stop Tygon® E-Lab Tubing, 1.22 mm ID, 12/pack, Red/Grey 72-0662 3-Stop PVC Tubing, 1.14 mm ID, Red-Red, pkg. of 6
73-2798 Aortic Cannula with Luer Taper for Mice, OD 1.0 mm 73-2798 Aortic Cannula with Luer Taper for Mice, OD 1.0 mm
73-2868 Aortic Metal Cannula with Luer Taper for Small Rat, OD 2.0 mm 73-2868 Aortic Metal Cannula with Luer Taper for Small Rat, OD 2.0 mm
73-2871 Aortic Metal Cannula with Luer Taper for Rat/Guinea Pig, OD 3.0 mm 73-2871 Aortic Metal Cannula with Luer Taper for Rat/Guinea Pig, OD 3.0 mm

Setup & Operation

The EasyCell System is a turnkey ex vivo heart perfusion system for cardiomyocyte isolation that is simple to setup, operate and maintain. Pressure monitoring can be added so that users can adjust flow rate to maintain a physiologic perfusion pressure.

Its compact design has a minimal footprint, conserving valuable bench space.

The heart is first perfused with a warmed, aerated standard perfusion buffer for blanching/clearing the heart of blood and then with a warmed, aerated protease solution for tissue disintegration. Unlike the PSCI system, the clearing buffer and collagenase share the same perfusion line, slightly delaying the delivery of the collagenase after switching over from the clearing buffer. The transition from clearing buffer to collagenase is accomplished by either moving the suction tube or turning a stopcock.

The EasyCell system has a single perfusion pathway that is fully compatible with ethanol for disinfection. The wetted components of the perfusion circuit can be flushed with a 70% ethanol solution or autoclaved for sterilization purposes should the cells need to be incubated for a number of hours following isolation rather than for immediate use.

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Primary Cell Isolation from Other Organs

This perfusion system has been specifically created to isolate primary cells from organs of species in the size range of mouse to guinea pig. The system can be configured for either in situ or ex vivo organ perfusion.

Features & Benefits

  • Simple to set up, operate and maintain
  • Compact size minimizes bench space required
  • Components of the perfusion circuit that come in contact with the perfusate can be flushed with 70% ethanol or autoclaved to sterilize

Applications

  • Cell isolation from mouse, rat or guinea pig organs by disintegration using protease solutions
  • Designed for cardiomyocyte isolation
  • Can be used with other organs ex vivo or in situ

Setup and Operation

The system is first used with perfusate for the removal of red blood cells (blanching) and then with the protease solution for tissue disintegration. The perfusate for blanching is warmed and oxygenated in a separate buffer reservoir and supplied via a peristaltic pump and warming coil. The protease solution is in a separate reservoir, oxygenated, and stirred.

For in situ perfusion, an operating table can be placed just below the warming coil, for ex vivo applications (heart, liver, etc.) the system can be equipped with a jacketed chamber.

Harvard Apparatus offers a complete line of perfusion cannulae (aortic, ex vivo andin situ) to accommodate vessels from 1.0 to 3.5 mm ID. The ex vivo and in situ cannulae feature our unique tip basket to prevent vessel occlusion.

Included items

 Base stand with clamps, bubble trap/Windkesselvessel, heating coil and protease reservoir

Additional equipment required

Thermocirculator, peristaltic pump, magnetic stirrer, reservoir, cannulae, operating table, transducer,monitoring system setup using the PLUGSYS Amplifier System

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