Culture Dish Incubator (DH-35iL)

Culture Dish Incubator (DH-35iL)

Designed to provide complete environmental control over 35 mm culture dishes while maintaining the ability to make both short- and long-term imaging studies.

  • Designed for glass-bottomed 35 mm cell culture dishes
  • Permits imaging, temperature, and gas environment control
  • Compatible with Corning, Falcon, MatTek, Nunc, WillcoWells and WPI Dishes
  • Unique dish clamps permit easy cell access
  • Compatible withWarner’s RC-37 Cell Culture Dish Perfusion Chamber Inserts

Item# Description U.S. List Price Quantity
64-0349 Culture Dish Incubation System DH-35i
64-1696 (CS-40R15) Round cover glass, #1.5 thickness, 40 mm, 50 pack
64-1543 (HCS-1) Heated top coverslip for DH-35i and DH-40iL chamber
64-0347 Chamber Insert for Willco/Corning 35 mm Dishes
64-0348 Chamber Insert for Falcon/Corning 35 mm Dishes
64-1922 (LCS-1) Liquid Cooling System
64-2400 TC-324C Heater Controller, Single Channel
64-2401 TC-344C Heater Controller, Dual Channel
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0102 SH-27B Solution In-Line Heater
64-0103 SF-28 Slow Flow In-Line Solution Heater
64-2342 (GHS-250) Gas humidification system
64-0351 Replacement Adapter Ring Kit for 35 mm Dishes
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-1544 (HCS-RPL) Replacement ITO coverslip for HCS-1 heated chamber top
64-1507 (RPC-2) Replacement clamps for DH-35i, DH-40iL, QE-1
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The introduction of glass-bottomed 35 mm culture dishes extended their use into areas such as confocal microscopy, fluorescence microscopy, and high resolution image analysis. The DH-35iL Culture Dish Incubator is a system designed for imaging live cells in an open culture dish (for easy access) or in a closed dish (for gas atmosphere control).

DH-35i Incubators work well with 35 mm culture dishes from all suppliers. Adapter rings are supplied to ensure a close fit for the supported dish. Dishes can be placed in the holder with or without dish covers.

The culture dish holder is machined in three pieces. The midsection and top are easily removed for dish replacement and enables the positioning of electrodes at low angles. Resistive heating of the DH-35iL is provided via the optional Warner TC-324C and TC-344C Temperature Controllers and a CC-28 cable.

Clamps are provided to ensure good contact between the dish and the heated surface and to prevent unwanted movement. The removable midsection is magnetically secured and has several ports to allow multi-channel perfusion, atmospheric maintenance, and aspiration of the dish. The base of the holder has a 25 mm aperture and the removable cover features a 36 mm glass aperture*.

When combined with Warner's RC-37 Cell Culture Perfusion Chamber Insert, a complete micro-environmental system is achieved, and this system is compatible with our SH-27B and SF-28 In-line Solution Heaters. Included with the system are an MP-2 perfusion manifold and a set of 6 culture dish adapter rings. Series 20 Stage adapters are available for all major microscopes. Custom or modified stage adapters are also available; contact our technical support department for details.

*Distance between bottom and top cover glass is 18.3 mm.

RC-37 Insert shown but purchased separately

AR-4 Ring Dimensions
ColorWeightID OD
Gold 1.3 g 38.96 mm




40.62 mm

Green 3.2 g 35.94 mm
Blue 2.7 g 33.81 mm
Red 2.1 g 37.44 mm
Black 3.4 g 35.46 mm
Silver 3.5 g 35.41 mm
  1. Extracellular ATP dissociates nonmuscle myosin from P2X7 complex: this dissociation regulates P2X7 pore formation. Ben J Gu, Catherine Rathsam, Leanne Stokes, Andrew B McGeachie, and James S. Wiley. Am J Physiol Cell Physiol (June 3, 2009).
  2. Talin phosphorylation by Cdk5 regulates Smurf1-mediated talin head ubiquitylation and cell migration. Huang C, Rajfur Z, Yousefi N, Chen Z, Jacobson K, Ginsberg MH. Nat Cell Biol. 2009 May;11(5):624-30. Epub 2009 Apr 12.
  3. Single-Cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary. Wang M, Orwar O, Weber SG. Anal Chem. 2009 May 15;81(10):4060-7.
  4. Retinoic Acid-induced Gene-1 (RIG-I) Associates with the Actin Cytoskeleton via Caspase Activation and Recruitment Domain-dependent Interactions. Mukherjee A, Morosky SA, Shen L, Weber CR, Turner JR, Kim KS, Wang T, Coyne CB. J Biol Chem. 2009 Mar 6;284(10):6486-94. Epub 2009 Jan 3.
  5. Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopy. Chan JW, Winhold H, Corzett MH, Ulloa JM, Cosman M, Balhorn R, Huser T. Cytometry A. 2007 Jul;71(7):468-74.
  6. Effect of Cell Size and Shape on Single-Cell Electroporation . Agarwal A, Zudans I, Weber EA, Olofsson J, Orwar O, Weber SG. Anal Chem. 2007 May 15;79(10):3589-96. Epub 2007 Apr 20.
  7. Experimentally Determining the IR Drop in Solution at Carbon Fiber Microelectrodes with Current Interruption and Application to Single-Cell Electroporation. Lambie BA, Brennan C, Olofsson J, Orwar O, Weber SG. Anal Chem. 2007 May 15;79(10):3771-8. Epub 2007 Apr 6.
  8. Simultaneous maximization of cell permeabilization and viability in single cell electroporation using an electrolyte-filled capillary. Agarwal A, Zudans I, Orwar O, Weber SG. Anal Chem. 2007 Jan 1;79(1):161-7.
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
DH-35iL (101213.1).pdfDH-35iL User's Manual