Flow Measurement Options for IH-SR and IH-5 in Langendorff and Working Heart Modes

TTFM-2 Module

Flow measurement can be accomplished in a functional IH system using two methods:

  • Indirect measurement by controlling the pump speed of a roller pump (SCP controller)
  • Direct measurement with ultrasound transit time technique (TTFM-2 module)

Required components are included in the IH-SR or IH-5 Core Systems and the selected IH-SR or IH-5 Core Systems Options. Species-specific flow probes and adapters required are described in the table in the Full Description.

To ensure that your system is properly configured as a functional unit that meets your application needs, please complete our Checklist and then contact Technical Services before placing an order. In the US and outside of Europe, call 800-547-6766 or email support@hbiosci.com. In Europe, please call (49) 7665-92000 or email sales@hugo-sachs.de.

Item# Description U.S. List Price Quantity
73-2806 Servo Controller for Perfusion (SCP)
73-4617 Transit Time Flowmeter Module (TTFM-2)
73-4668 MA1PRB Flow probe for TTFM-2 or TS420 Module
73-3554 Adapter for 73-4668 1RB Flow Probe for IH-SR
73-4644 MA2PSB Flow probe for TTFM-2 or TS420 Module
73-2819 Adapter for 73-4644 2SB Flow Probe for IH-SR
73-4647 MA2.5PSB Flow Probe for TTFM-2 or TS420 Module
73-2820 Adapter for 73-4647 2.5SB Flow Probe for IH-SR
73-3071 Adapter for 73-4647 F2.5SB Flow Probe for IH-5
73-4652 MA4PSB Flow Probe for TTFM-2 or TS420 Module
73-3069 Adapter for 73-4652 4SB Flow Probe for IH-5
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Flow measurement can be accomplished in a functional IH system using two methods:

  • Indirect measurement by controlling the pump speed of a roller pump (SCP controller)
  • Direct measurement with Ultrasound Transit Time Technic (TTFM-2 module)

Applications

  • Accurate coronary flow measurement on drug studies
  • Studies on coronary resistance changes (pre and post ischemia)
  • Myogene autoregulation (reactive hyperemia)


Constant Pressure/Flow Control

If you do "Langendorff only", the PLUGSYS Servo Controller Module (SCP) is used in combination with a roller pump to perfuse the heart in constant pressure mode. The SCP module calculates the coronary flow from the pump speed of the roller pump (indirect flow measurement). The PLUGSYS TAM-D module amplifies the measured perfusion pressure signal and outputs it to the data acquisition system. Both the roller pump and the TAM-D are included in the IH-SR and IH-5 Core Systems.

  • Allows perfusion under constant pressure or constant flow (direct switchable)
  • Provides accurate control of perfusion pressure or flow, even at very low flow rates
  • Indirect coronary flow measurement from pump speed


Direct Flow Measurement with Ultrasound Transit Time Flowmeter

The PLUGSYS TTFM-2 module, in combination with a Starling Pressure Controller, provides real and accurate transit time flow measurement in ml/min for direct coronary flow measurement in retrograde Langendorff mode or aortic flow measurement in working ejecting heart. In addition, species-specific flow probes and adapters are required.

Features & Benefits

  • Accurate ultrasonic transit time direct flow measurement
  • Unique integration of flow probe into perfusion path
  • Laminar flow lines improve accuracy of flow measurement
  • Thermal properties of Perspex adapter reduce temperature loss
  • Increased system flexibility by allowing the study of Myogene Autoregulation (Reactive Hyperemia)
  • Indirect flow measurement can typically follow increasing flow rates but often lags behind decreasing flow rates

The table below specifies the flow probe and adapter to be used for each type of isolated heart system (IH-SR, IH-5 or UP-100IH) for the indicated application. It is extremely important to order the correct probe and adapter pair. If ordering online, please select carefully from the item listing or contact technical support for assistance.

Type of Measurement/Species Required Probe and Adapter
Direct Flow Measurement/Mouse with IH-SR
Application: Coronary flow measurement in retrograde Langendorff mode or aortic flow measurement in working heart. 
 73-4668  MA1PRB Flow Probe for TTFM-2
 73-3554  Adapter for 73-4668 1RB Flow Probe for IH-SR (flow probe is embedded into this adapter)
Direct Flow Measurement/Rat with IH-SR
Application: Coronary flow measurement in retrograde Langendorff mode or aortic flow measurement in working heart.
73-4644 MA2PSB Flow Probe for TTFM-2
73-2819 Adapter for 73-4644 2SB Flow Probe for IH-SR (flow probe is embedded into this adapter)
Direct Flow Measurement/Rat and Guinea Pig with IH-5
Application: Coronary flow measurement in retrograde Langendorff mode or aortic flow measurement in working heart.

73-4647 MA2.5PSB Flow Probe for TTFM-2 or TS420 Module
73-3071 Adapter for 73-4647 F2.5SB Flow Probe for IH-5

Direct Flow Measurement/Rabbit with IH-5
Application: Coronary flow measurement in retrograde Langendorff mode or aortic flow measurement in working heart.
73-4652 MA4PSB Flow Probe for TTFM-2 or TS420 Module
73-3069 Adapter for 73-4652 4SB Flow Probe for IH-5
Cardiac Output Measurement, Working Heart/Mouse with IH-SR
Application: Flow measurement into left atrium in Working Heart mode
73-4668  MA1PRB Flow Probe for TTFM-2
73-3554  Adapter for 73-4668 1RB Flow Probe for IH-SR (flow probe is embedded into this adapter)
Cardiac Output Measurement, Working Heart/Rat with IH-SR
Application: Flow measurement into left atrium in Working Heart mode
73-4647 MA2.5PSB Flow Probe for TTFM-2
73-2820 Adapter for 73-4647 2.5SB Flow Probe for IH-SR (flow probe is embedded into this adapter)
Cardiac Output Measurement, Working Heart/Rat and Guinea Pig with IH-5
Application: Flow measurement into left atrium in Working Heart mode
73-4647 MA2.5PSB Flow Probe for TTFM-2 or TS420 module
73-3071 Adapter 73-4647 2.5SB Flow Probe 2.5SB for IH-5
Cardiac Output Measurement, Working Heart, Rabbit with IH-5
Application: Flow measurement into left atrium in Working Heart mode
73-4652 MA4PSB Flow Probe for TTFM-2 or TS420 Module
73-3069 Adapter for 73-4652 4SB Flow Probe for IH-5
Specifications73-2806
AccessoriesOperating Instructions, Screwdriver, Output Cable to Pump
Actual Value Input0 - 10 V via System Bus
Ambient ConditionsLaboratory Indoors
ConnectorDIN 41612, 96-pin VG
ConstructionPlug-in Module for a PLUGSYS
Control Gain1 - 50, Adjustable
Control Voltage0 - 10 V to Operate the Pump
Derivative Factor0.1 - 100, Adjustable
DescriptionServo Controller for Perfusion (SCP) Type 704
Digital IndicationRange -1999 to +1999, Selectable for Actual Value, Setpoint, or Control Output Signal
FunctionElectronic PID Controller for Physiological and Pharmacological Research
Integral Factor0.05 - 10, Adjustable
PLUGSYS Width*2 slot units
Power Supply5 V, 0.3 A from PLUGSYS
Recorder Output0 - 10 V, BNC Socket
Setpoint0 - 5 V, Adjustable
Weight (Metric)0.3 kg