Cation Exchange Chromatography SpinColumns

Cation Exchange Chromatography Spin Columns

Cation Exchange Columns are filled with a negatively charged resin to attract positively charged molecules from samples.

Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.

Item# Description U.S. List Price Quantity
74-7235 Ultra-Micro SpinColumn, Strong Cation SP, Pkg. 24
74-7215 Ultra-Micro SpinColumn, Strong Cation SP, Pkg. 96
74-4426 Ultra-Micro SpinColumn, Strong Cation SA, Pkg. 24
74-4425 Ultra-Micro SpinColumn, Strong Cation SA, Pkg. 96
74-7236 Ultra-Micro SpinColumn, Weak Cation CM, Pkg. 24
74-7216 Ultra-Micro SpinColumn, Weak Cation CM, Pkg. 96
74-4427 Ultra-Micro SpinColumn, Weak Cation AA, Pkg. 96
74-4706 Micro SpinColumn, Strong Cation SP, Pkg. 24
74-4702 Micro SpinColumn, Strong Cation SP, Pkg. 96
74-4413 Micro SpinColumn, Strong Cation SA, Pkg. 24
74-4412 Micro SpinColumn, Strong Cation SA, Pkg. 96
74-4707 Micro SpinColumn, Weak Cation CM, Pkg. 24
74-4703 Micro SpinColumn, Weak Cation CM, Pkg. 96
74-4415 Micro SpinColumn, Weak Cation AA, Pkg. 24
74-4414 Micro SpinColumn, Weak Cation AA, Pkg. 96
74-4206 Macro SpinColumn, Strong Cation SP, Pkg. 24
74-4202 Macro SpinColumn, Strong Cation SP, Pkg. 96
74-4153 Macro SpinColumn Strong Cation SA, Pkg. 24
74-4152 Macro SpinColumn, Strong Cation SA, Pkg. 96
74-4207 Macro SpinColumn, Weak Cation CM, Pkg. 24
74-4203 Macro SpinColumn, Weak Cation CM, Pkg. 96
74-4155 Macro SpinColumn, Weak Cation AA, Pkg. 24
74-4154 Macro SpinColumn, Weak Cation AA, Pkg. 96
74-5625 96-Well Micro SpinColumn, Strong Cation SP, 1 plate
74-5632 96-Well Micro SpinColumn, Strong Cation SA, 1 plate
74-5627 96-Well Micro SpinColumn, Weak Cation CM, 1 plate
74-5665 96-Well Macro SpinColumns, Strong Cation SP, 1 plate
74-5672 96-Well Macro SpinColumns, Strong Cation SA, 1 plate
74-5667 96-Well Macro SpinColumns, Weak Cation CM, 1 plate
74-5674 96-Well Macro SpinColumns, Weak Cation AA, 1 plate
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Cation Exchange Columns are filled with a negatively charged resin to attract positively charged molecules from samples.

Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.

Available Matrices (Packing Materials)

Matrix Functional Group pH Range Ionic Capacity
Strong Cation SA*      
Strong Cation SP**  Sulfopropyl  6 - 10   0.18 - 0.25 mmol (Cl-)/ml
Weak Cation AA***    
Weak Cation CM****  Carboxymethyl 
 5  - 9  0.09 - 0.13 mml (Cl-)/ml

*SA =    **SP = Fast Flow Sepharose ***AA =  ****CM = Cellulose Sephaose

About Ion Exchange Chromatography

Ion exchange chromatography (IEX) is an effective method of sample purification and fractionation based on molecular charge. Samples are passed through spin columns containing a predetermined matrix. Target molecules are captured and held by ionic bonds to the insoluble stationary matrix while the remaining sample is allowed to flow through the column. The ionic bonds are then broken through the addition of an elution buffer which changes the pH and alters the ionic strength of the molecules being retained. The bound molecules are eluted in order of the least to most strongly bound allowing for the collection of samples in fractions for separate analysis.

Specifications
Column Type Sample Volume Bed Volume Sample Concentration Included Choice of Packing Materials (Matrices)
Ultra-Micro  10 µl - 25 µl 37.96 µl 3 - 30 µg

Two 2 ml centrifuge tubes with top caps

Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 2 ml centrifuge tubes with top caps Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
Macro  75 µl - 150 µl 191.45 µl
30- 300 µg
Two 2 ml centrifuge tubes with top and bottom caps Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
96-Well Micro  25 µl - 75 µl 66.42 µl
5 - 60 µg
Two 96-well collection plates (1.1 ml per well) Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
96-Well Macro  25 µl - 150 µl 191.45 µl
30- 300 µg
Two 96-well collection plates (1.1 ml per well) Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP