Cation Exchange Chromatography SpinColumns

Cation Exchange Chromatography SpinColumns

QuikPrep® SpinColumns™ for cation exchange chromatography are filled with a negatively charged resin to attract positively charged molecules from samples.

Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.

Item# Description U.S. List Price Quantity
74-7235 Ultra-Micro SpinColumn, Strong Cation SP, Qty. of 24
74-7215 Ultra-Micro SpinColumn, Strong Cation SP, Qty. of 96
74-4706 Micro SpinColumn, Strong Cation SP, Qty. of 24
74-4702 Micro SpinColumn, Strong Cation SP, Qty. of 96
74-4206 Macro SpinColumn, Strong Cation SP, Qty. of 24
74-4202 Macro SpinColumn, Strong Cation SP, Qty. of 96
74-5625 96-Well Micro SpinColumn, Strong Cation SP, 1.1 ml Reservoir Plate (1)
74-5665 96-Well Macro SpinColumns, Strong Cation SP, 1.1 ml Reservoir Plate (1)
74-4426 Ultra-Micro SpinColumn, Strong Cation SA, Qty. of 24
74-4425 Ultra-Micro SpinColumn, Strong Cation SA, Qty. of 96
74-4413 Micro SpinColumn, Strong Cation SA, Qty. of 24
74-4412 Micro SpinColumn, Strong Cation SA, Qty. of 96
74-4153 Macro SpinColumn Strong Cation SA, Qty. of 24
74-4152 Macro SpinColumn, Strong Cation SA, Qty. of 96
74-5632 96-Well Micro SpinColumn, Strong Cation SA, 1.1 ml Reservoir Plate (1)
74-5672 96-Well Macro SpinColumns, Strong Cation SA, 1.1 ml Reservoir Plate (1)
74-7236 Ultra-Micro SpinColumn, Weak Cation CM, Qty. of 24
74-7216 Ultra-Micro SpinColumn, Weak Cation CM, Qty. of 96
74-4707 Micro SpinColumn, Weak Cation CM, Qty. of 24
74-4703 Micro SpinColumn, Weak Cation CM, Qty. of 96
74-4207 Macro SpinColumn, Weak Cation CM, Qty. of 24
74-4203 Macro SpinColumn, Weak Cation CM, Qty. of 96
74-5627 96-Well Micro SpinColumn, Weak Cation CM, 1.1 ml Reservoir Plate (1)
74-5667 96-Well Macro SpinColumns, Weak Cation CM, 1.1 ml Reservoir Plate (1)
74-4427 Ultra-Micro SpinColumn, Weak Cation AA, Qty. of 96
74-4415 Micro SpinColumn, Weak Cation AA, Qty. of 24
74-4414 Micro SpinColumn, Weak Cation AA, Qty. of 96
74-4155 Macro SpinColumn, Weak Cation AA, Qty. of 24
74-4154 Macro SpinColumn, Weak Cation AA, Qty. of 96
74-5674 96-Well Macro SpinColumns, Weak Cation AA, 1.1 ml Reservoir Plate (1)
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QuikPrep® SpinColumns™ for cation exchange chromatography are filled with a negatively charged resin to attract positively charged molecules from samples.

Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.

Available Matrices (Packing Materials)

Matrix Functional Group pH Range Ionic Capacity Applications
Strong Cation SA
Poly 2-sulfoethyl aspartamide 2.7 to 4.0 0.4 to 0.5 mmol (K+)/g High molecular weight protein 
separation
Strong Cation SP Sulfopropyl 6 to 10 0.18 to 0.25 mmol (Na+)/ml Protein separation
Weak Cation AA Poly (aspartic acid) 4 to 8 0.4 to 0.5 mmol (triethylammonium ion)/g Protein separation
Weak Cation CM Carboyxmethyl 5 to 9 0.09 to 0.13 mmol (Na+)/ml Protein separation

SA = Polysulfoethyl A™ (silica based)   SP = SP-Sepharose Fast Flow   AA = PolyCAT A™ (poly aspartic acid attached to silica)   CM = Carboxymethyl-cellulose


About Ion Exchange Chromatography

Ion exchange chromatography (IEX) is an effective method of sample purification and fractionation based on molecular charge. Samples are passed through spin columns containing a predetermined matrix. Target molecules are captured and held by ionic bonds to the insoluble stationary matrix while the remaining sample is allowed to flow through the column. The ionic bonds are then broken through the addition of an elution buffer which changes the pH and alters the ionic strength of the molecules being retained. The bound molecules are eluted in order of the least to most strongly bound allowing for the collection of samples in fractions for separate analysis.

Specifications

Available Matrices (Packing Materials)

Matrix Functional Group pH Range Ionic Capacity Applications
Strong Cation SA
Poly 2-sulfoethyl aspartamide 2.7 to 4.0 0.4 to 0.5 mmol (K+)/g High molecular weight protein 
separation
Strong Cation SP Sulfopropyl 6 to 10 0.18 to 0.25 mmol (Na+)/ml Protein separation
Weak Cation AA Poly (aspartic acid) 4 to 8 0.4 to 0.5 mmol (triethylammonium ion)/g Protein separation
Weak Cation CM Carboyxmethyl 5 to 9 0.09 to 0.13 mmol (Na+)/ml Protein separation

SA = Polysulfoethyl A™ (silica based)   SP = SP-Sepharose Fast Flow   AA = PolyCAT A™ (poly aspartic acid attached to silica)   CM = Carboxymethyl-cellulose

SpinColumn Types

Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
Two 96-well collection plates (1.1 ml per well) Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
Two 96-well collection plates (1.1 ml per well) Weak Cation AA, Weak Cation CM, Strong Cation SA, Strong Cation SP
           



DOCUMENT DESCRIPTION
QuikPrep Ultra Micro SpinColumn QSG 9511-042 Rev 2.0.pdf'Ultra-Micro SpinColumns Quick Start Guide
QuikPrep Micro SpinColumn QSG 9511-036 Rev 2.0-v1.pdf'Micro SpinColumns Quick Start Guide
QuikPrep Macro SpinColumn QSG 9511-032 Rev 2.0.pdf'Macro SpinColumns Quick Start Guide
QuikPrep 96 Well SpinColumns QSG 9511-033 Rev 2.0.pdf'96-Well SpinColumns Quick Start Guide