Anion Exchange Chromatography SpinColumns

Anion Exchange Chromatography SpinColumns

Anion Exchange SpinColumns are filled with a positively charged resin to attract negatively charged molecules from samples.

Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.

Item# Description U.S. List Price Quantity
74-7233 Ultra-Micro SpinColumn, Strong Anion Q, Qty. of 24
74-7213 Ultra-Micro SpinColumn, Strong Anion Q, Qty. of 96
74-4704 Micro SpinColumn, Strong Anion Q, Qty. of 24
74-4700 Micro SpinColumn, Strong Anion Q, Qty. of 96
74-4204 Macro SpinColumn, Strong Anion Q, Qty. of 24
74-4200 Macro SpinColumn, Strong Anion Q, Qty. of 96
74-5624 96-Well Micro SpinColumn, Strong Anion Q, 1.1 ml Reservoir Plate (1)
74-5664 96-Well Macro SpinColumns, Strong Anion Q, 1.1 ml Reservoir Plate (1)
74-7214 Ultra-Micro SpinColumn, Weak Anion DEAE, Qty. of 96
74-7234 Ultra-Micro SpinColumn, Weak Anion DEAE, Qty. of 24
74-4705 Micro SpinColumn, Weak Anion DEAE, Qty. of 24
74-4701 Micro SpinColumn, Weak Anion DEAE, Qty. of 96
74-4205 Macro SpinColumn, Weak Anion DEAE, Qty. of 24
74-4201 Macro SpinColumn, Weak Anion DEAE, Qty. of 96
74-5626 96-Well Micro SpinColumn, Weak Anion DEAE, 1.1 ml Reservoir Plate (1)
74-5666 96-Well Macro SpinColumns, Weak Anion DEAE, 1.1 ml Reservoir Plate (1)
74-4423 Ultra-Micro SpinColumn, Weak Anion Linear PEI, Qty. of 96
74-4411 Micro SpinColumn, Weak Anion Linear PEI, Qty. of 24
74-4410 Micro SpinColumn, Weak Anion Linear PEI, Qty. of 96
74-4151 Macro SpinColumn, Weak Anion Linear PEI, Qty. of 24
74-4150 Macro SpinColumn, Weak Anion Linear PEI, Qty. of 96
74-5633 96-Well Micro SpinColumn, Weak Anion Linear PEI, 1.1 ml Reservoir Plate (1)
74-5673 96-Well Macro SpinColumns, Weak Anion Linear PEI, 1.1 ml Reservoir Plate (1)
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Anion Exchange SpinColumns are filled with a positively charged resin to attract negatively charged molecules from samples.

Matrices are available in either strong or weak ionized states. This expresses the ability of the exchange matrix to maintain its charge with a change in pH and not the strength of the bond. Strong exchangers have functional groups that will always remain ionized, while the functional groups of the weak exchangers can be neutralized by changing the pH.

Available Matrices (Packing Materials)

Matrix Functional Group pH Range Ionic Capacity
Strong Anion Q*
 Quaternary Ammonium  2 - 9  0.18 - 0.25 mmol (Cl-)/ml
Weak Anion DEAE**
 Diethylaminoethyl  5 - 9   0.11 - 0.16 mmol (Cl-)/ml
Weak Anion PEI***
 Polyethylenimine 
 

*Q-Sepharose **Diethylaminoethyl-cellulose ***Polyethylenimine Polywax PL

About Ion Exchange Chromatography

Ion exchange chromatography (IEX) is an effective method of sample purification and fractionation based on molecular charge. Samples are passed through spin columns containing a predetermined matrix. Target molecules are captured and held by ionic bonds to the insoluble stationary matrix while the remaining sample is allowed to flow through the column. The ionic bonds are then broken through the addition of an elution buffer which changes the pH and alters the ionic strength of the molecules being retained. The bound molecules are eluted in order of the least to most strongly bound allowing for the collection of samples in fractions for separate analysis.

Specifications
Column Type Sample Volume Sample Capacity Suggested
Elution Volume
Included Choice of Packing Materials
(Matrices)
Ultra-Micro  10 µl to 25 µl 3 to 30 µg
 28.5 µl

Two 2 ml centrifuge tubes with top caps

Strong Anion Q, Weak Anion DEAE, Weak Anion PEI
Micro  25 µl to 75 µl 5 to 60 µg
 50 µl
Two 2 ml centrifuge tubes with top caps Strong Anion Q, Weak Anion DEAE, Weak Anion PEI
Macro  75 µl to 150 µl 30 to 300 µg
 143 µl
Two 2 ml centrifuge tubes with top and bottom caps Strong Anion Q, Weak Anion DEAE, Weak Anion PEI
96-Well Micro  25 µl to 75 µl 5 to
60 µg
 50 µl
Two 96-well collection plates (1.1 ml per well) Strong Anion Q, Weak Anion DEAE, Weak Anion PEI
96-Well Macro  25 µl to
150 µl
30 to
300 µg
 143 µl
Two 96-well collection plates (1.1 ml per well) Strong Anion Q, Weak Anion DEAE, Weak Anion PEI

Q = Q Sepharose (Quaternary amine sepharose)    
DEAE = DEAE Cellulose Sepharose
PEI = Polywax LP (silica based)