Zbicz Top (BSC-ZT)

Zbicz Top (BSC-ZT)

The BSC-ZT Zbicz Top is a submersion-type chamber in which tissue slices are supported on a stiff nylon mesh. Slices are perfused from below and above.

  • Slices remain viable for 10 hours or more
  • Slices are mechanically stable
  • Low volume chamber cavity
  • Slices are wetted on both top and bottom and do not dry out
  • Semipermanent mesh slice support is easily cleaned
  • Low electrode capacitance assists single microelectrode voltage and patch clamping
  • Minimal bubble formation
  • Precise solution temperature regulation
  • Slice can be trans-illuminated

Item# Description U.S. List Price Quantity
64-1525 (BSC-BUW) BSC-BU base unit compatible with Warner temperature controllers, includes connecting cable
65-0074 Model BSC-ZT (Zbicz Top) for Tissue Slice Chamber
65-0075 Model BSC-HT (Haas Top) for Tissue Slice Chamber
65-0076 Model BSC-PC Prechamber (for holding slices) Tissue Slice Chamber
64-0100 (TC-324B) Heater Controller, single channel, 110-120 VAC, 60Hz
64-0101 (TC-344B) Heater Controller, dual channel, 110-120 VAC, 60Hz
65-0073 Model BSC-BU (Base Unit) Tissue Slice Chamber
65-0045 Model TC-202A Bipolar Temperature Controller for use with PDMI-2, PSMI, CSMI, LU-CB-1, LU-CPC-CEH, and BSC-BU.
65-0078 Model BSC-AIR Aeration Element Replacement, Set of 2
65-0081 Model BSC-TUBE Base Unit (Model BSC-BU) Tubing Set Replacement, Tygon .031 x .093 (ID x OD)
65-0079 Model BSC-ORING Base Unit (Model BSC-BU) Replacement Gasket Set
65-0082 Model BSC-G10 Haas Top (Model BSC-HT) Tissue Insert Replacement, Set of 2
65-0083 Model BSC-HTC Haas Top (Model BSC-HT) Plastic Cover Replacement
65-0084 Model BSC-HT-AG Haas Top (Model BSC-HT) Ground Wire Replacement
65-0086 Model BSC-ZTC Zbicz Top (Model BSC-ZT) Plastic Insert Replacement
65-0087 Model BSC-PC-VAL Prechamber (Model BSC-PC) Valve Replacement
65-0608 Insert for Model BSC-PC Prechamber Tissue Slice Chamber
65-0090 Model BSC-MESH Haas Top (Model BSC-HT) Zbicz Top (Model BSC-ZT) Replacement Mesh 300 µm grid, 5 x 5 in, pkg. of 4
65-0088 Model BSC-ACC-KIT Base Unit, Haas & Zbicz Tops Accessory Kit (Replacement Screws, Fittings and Mesh)
64-1697 (BSC-HEW) Replacement heating wire for BSC-BUW
64-1591 (BSC-CABLE-W) BSC=BU connecting cable for Warner temperature controllers
65-0080 Model BSC-HE Base Unit Heating Element Replacement
65-0091 Model BSC-CABLE Base Unit (Model BSC-BU)/Temperature Controller (Model TC-102) Replacement Power Cable
65-0104 Output Cable, 6-Pin to Banana for 65-0045 Model TC-202A or 65-0073 Model BSC-BU
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The Zbicz Top is a submersion-type chamber in which tissue slices are supported on a stiff nylon mesh. Slices are perfused both from below and above.

  • Slices remain viable for 10 hours or more
  • Slices are mechanically stable
  • Low volume chamber cavity
  • Slices are wetted on both top and bottom and do not dry out
  • Semipermanent mesh slice support is easily cleaned
  • Low electrode capacitance assists single microelectrode voltage and patch clamping
  • Minimal bubble formation
  • Precise solution temperature regulation
  • Slice can be trans-illuminated

Slices remain viable for 10 hours or more

The Zbicz Top (BSC-ZT) is a submersion-type chamber in which tissue slices are supported on a nylon mesh. The perfusate flows transversely across both cut surfaces. The steady flow of warmed media across the slightly submerged slice surfaces is sufficient to keep many tissues alive and responsive for 10 or more hours.

The fluid level is set to 1 mm above the mesh by a dam at the end of the chamber. The chamber is 6 mm wide and the trough is 24 mm long; this provides adequate room for slices and instrumentation, yet minimizes the well volume to optimize drug change over times. Fluid overflowing the dam is drained via a vacuum line to waste.

Slices are mechanically stable

Slices are placed on a special ribbed nylon mesh and may be held in place by 3 to 4 stimulating electrodes or a plastic insert. This stability is attributed to the stiff nylon mesh which does not rise or sag with changes in fluid flow, therefore moderate changes in the transverse laminar flow can be well tolerated during intracellular and extracellular recordings. These features allow for switching of solutions with minimal risk of tissue movement and loss of neurons while recording.

Low volume chamber

The low volume of the chamber cavity and the relatively fast flow rate allows rapid exchange of solution (1 to 2 minutes) which facilitates drug application studies.

Slices remain moist

Slices remain moist and viable, because they are wetted on both sides and do not dry out due to surrounding air currents.

Semipermanent mesh

The mesh surface holding the slice is semipermanent and can be cleaned easily. The BSC-ZT comes with extra mesh which can be installed easily when necessary.

Low electrode capacitance

Because the fluid depth is shallow (400 to 600 microns), the electrode capacitance remains low; an important consideration in single microelectrode voltage and patch clamping.

Minimal bubble formation

Bubbles, which can form in the heating unit, escape by the path formed at the entrance of the trough. Movement of the slice will not result if bubbles inadvertently form under the mesh. The flow of solution over the slice top will maintain tissue viability.

Specifications
Dimensions, (L x W x H) 98.55 x 101.60 x 28.19 mm
(3.880 x 4.000 x 1.110 in)
Weight 124.74 g (4.40 oz)