Yeast Cell Chambers (YC-1/YC-2)

Yeast Cell Chambers (YC-1/YC-2)

Flow Chamber for Yeast Cells

  • Monitor yeast cell growth using time-lapse microscopy, while changing media
  • Cells grow two dimensionally over multiple cell cycles
  • Change media in seconds without washing cells out
  • Resistive heating or Peltier temperature control options

Item# Description U.S. List Price Quantity
64-1678 (YC-1) Flow chamber for yeast cells, resistive heaters
64-1679 (YC-2) Flow chamber for yeast cells, Peltier version
64-1680 (PH-10) Heated plaltform (resistive)
64-1682 (SIC-4) PDMS chambers, 4 pack
64-1684 (CME-50) Cellulose membranes, 50 pack
64-0752 PE-50/10 PE-50 Polyethylene Tubing, 10 ft L
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-1489 (SN-18) Blunt-ended syringe needles, 18G (1.27 mm OD), fits PE-160 tubing, 12 pack
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Research in cell biology often requires the microscopic study of individual live cells under various conditions, such as at different temperatures or in the presence of different growth media.

One particular interest is to monitor individual cell growth using time lapse microscopy while seamlessly and rapidly changing the liquid environment. The YC-1/YC-2 flow chambers have been developed to allow observation of yeast cells proliferating in a bi-dimensional manner over multiple cell cycles.

The YC-1/YC-2 chambers overcome a common problem encountered in standard time lapse setups (e.g., those using agar gel pads) in that these setups do not allow media to be changed during the experiment. In addition, standard flow chambers do not allow monitoring of multiple cell cycles since the progeny is eventually washed away by the flow.

YC-1 Chamber

The YC-1 is our standard time lapse chamber and is YC-1 Chamberoptimized for use at room temperature or above.

The principle components of this system includes:

  1. PDMS flow chamber
  2. PDMS coated coverslips
  3. Cellulose membranes
  4. Temperature controlled platform

Additional components required to complete the system are a TC-324C temperature controller, a fluid delivery system (e.g., VC-8), and a Series 20 microscope stage adapter. This chamber is designed for use on an inverted microscope.

The YC-1 is supplied with four PDMS microfluidic chambers, fifty PDMS coated coverslips, fifty cellulose membranes, one chamber platform with resistive heaters, ten feet of PE-160 tubing, and one package of 18 gauge blunt-end syringe needles.

YC-2 Chamber 

The YC-2 is similar to the YC-1 but incorporates a Peltier device allowing it to YC-2 Chamberbe used at temperatures below ambient.

As with the YC-1, the principle components of YC-2 include:

  1. PDMS flow chamber
  2. PDMS coated coverslips
  3. Cellulose membranes
  4. Temperature controlled platform

Additional components required to complete the system are a CL-100 temperature controller, an LCS-1 liquid cooling system, a fluid delivery system (e.g., VC-8), and a Series 20 microscope stage adapter. This chamber is also designed for use on an inverted microscope.

The YC-2 is supplied with four PDMS microfluidic chambers, fifty PDMS coated coverslips, fifty cellulose membranes, one chamber platform with integrated Peltier devices, ten feet of PE-160 tubing, and one package of 18 gauge blunt-end syringe needles.

Specifications
  YC-1 YC-2
Temperature Range Ambient to 50° 5° to 50°
Material Platform aluminum black anodized, polycarbonate cover
PDMS Flow cell dimensions 50 L x 23 W x 4.5 H mm
Bath dimensions (L x W x H) 40 x 0.6 x 0.1 mm
Platform Aperture (L x W) 9.5 x 13 mm
Bath volume 2.4 µl
Coverslips 24 x 50 mm #1 thickness with 40 µm PDMS coating
Cellulose membranes 24 x 50 mm x 30 µm thick
Perfusion ports 1.27 mm OD
  1. Long-term imaging in microfluidic devices. Charvin G, Oikonomou C, Cross FR. Methods Mol Biol., 591:229-42, 2010.

  2. Origin of irreversibility of cell cycle Start in budding yeast. Charvin G, Oikonomou C, Siggia ED, Cross FR. PLoS Biology, 8(1):e1000284, 2010.

  3. Forced periodic expression of G1 cyclins phase-locks the budding yeast cell cycle. Charvin G, Cross FR, Siggia ED. PNAS, 106(16):6632-7, 2009.
  4. A microfluidic device for temporally controlled gene expression and long-term fluorescent imaging in unperturbed dividing yeast cells. Charvin G, Cross FR, Siggia Ed. PLoS ONE, 3(1):e1468, 2008.
DOCUMENT DESCRIPTION
YC-1 (090428 prelim).pdfYeast Cell Chambers (YC-1) User's Manual
YC-2 (090723).pdfYeast Cell Chambers (YC-2) User's Manual