MicroPoration is a novel non viral method used to transfect difficult cells lines including primary cells. MicroPoration is a unique electroporation technology which uses a specially designed pipette TIP as the electroporation chamber. Typical electroporation is dependent on cuvette style chambers which are used routinely for electroporation. These pipette tips have been developed to produce a uniform electrical field and minimal heat generation within the chamber which result in higher transfection efficiencies and cell viabilities. Imagine your pipette tip is functionally equal to the electroporation cuvette! With the use of these tips smaller sample volumes of 10µl and 100µl can be transfected efficiently. With the fully programmable pulse generator you can simply pipette up your sample, place it in the pipette station, pulse, and then plate your cells. Fast and easy! Search for Product in current catalog
• Pipette sample, Pulse and Plate, Transfect Directly In Pipette Tip • High Efficiency and Cell Viability of Hard to Transfect Cells • 10µl -100µl Samples Now Possible Single Technology for All Cells • Optimized Protocols and User Control
The OneDrop MicroPorator System is a hand-held pipette-type electroporation device which utilizes powerful MicroPoration technology. It consists of a high performance pulse generator, safety chamber and MicroPorator pipette. Pre-sterilized microporation tips, manufactured by state-of-the-art micromachining factories, are supplied as disposables. The high performance pulse generator has a graphical user interface for easy setting and operation. Setting electrical parameters, number of pulses and pulse duration are graphically visualized with a large LCD display. Pre-optimized electric parameters are provided and updated through the technical support group.
Interleukin-12-mediated induction of systemic immunity in the perihpery and recruitment of activated T cells into the brain produce limited antitumor effects compared with interleukin-2