RC-20 and RC-20H are very small volume imaging chambers featuring fast perfusion exchange and short working distance. Two
15 mm diameter coverslips enclose the bath. Distance between top and bottom coverslips is 1 mm. The top coverslip is held in place with a retainer. The sample coverslip is placed on the chamber bottom and the assembled chamber is placed in the chamber platform.

Two bath sizes are available. The smaller and narrower bath of the RC-20 insures a linear flow and fast solution exchange while the larger RC-20H provides a larger viewing area The additional port at the bath input may be used to inject substances into the bath or to remove gas bubbles. Order with P-5 or PH-5 platform.

Series 20 Imaging Chambers use coverslips at both top and bottom to enclose the bath area. The closed bath promotes an even and continuous solution exchange and eliminates changes in focus due to alterations in bath height.

Studies involving physiological bicarbonate buffers can be performed with no gas loss at the chamber interface. The closed design allows the use of permeable supports. With a laminar flow, minimal distortion of the support will occur. Closed chambers have been used in fluorescent, calcium and time-lapse imaging studies.

All Series 20 chambers equipped with a stainless steel suction tube now include a suction tube clamp. This ensures the adjustable tube will not move once the solution height is set.