U9500 series Ussing chambers are machined from solid clear acrylic rod into two symmetric halves. Each half has locating holes on the outside end for mounting onto the support bracket. One face has sharp stainless-steel pins which mate with holes in the corresponding face for puncturing and positioning the epithelium membrane. Luer-tapered fittings are pre-installed in the vertical and horizontal ports for circulation of buffer solutions and for making electrical I/O connections into the chamber.
U9500 series Ussing chambers are available in five diameters from 3.8 mm to 13.5 mm and three slotted sizes to accommodate a variety of tissue sizes. The choice of chamber depends on the species and epithelia under study. The specified diameter designates the main chamber opening in which the buffer makes contact with the membrane.