The U9500 is a complete system consisting of Ussing chamber, support stand, circulation reservoir, ring stands, 3-prong clamp, air/gas fitting kit and electrode set.
U9500 series Ussing chambers are machined from solid clear acrylic rod into two symmetric halves. Each half has locating holes on the outside end for mounting onto the support bracket. One face has sharp stainless-steel pins which mate with holes in the corresponding face for puncturing and positioning the epithelium membrane. Luer-tapered fittings are pre-installed in the vertical and horizontal ports for circulation of buffer solutions and for making electrical I/O connections into the chamber.

U9500 series Ussing chambers are available in five diameters from 3.8 mm to 13.5 mm and three slotted sizes to accommodate a variety of tissue sizes. The choice of chamber depends on the species and epithelia under study. The specified diameter designates the main chamber opening in which the buffer makes contact with the membrane.

The use of permeable cell culture inserts (porous membrane filters, culture cups, and culture wells) has become widespread in recent years. The numerous advantages of this application have revolutionized in vitro cell biology and it is now used extensively in electrophysiological studies of epithelium. The U9521 Ussing chamber is designed to hold tissue culture inserts using low-cost adapters. The chamber is mounted in the standard support stand and procedures for experimentation are the same as with the "classic" Ussing system. This is a very versatile and economical system for using tissue culture inserts in transepithelial studies. Adapters in a large variety of sizes for most brands of culture inserts are available.
The circulation reservoir is hand-blown from borosilicate glass and is available in four fluid capacities. Two glass condensers are included to minimize evaporative losses. Reservoir openings are provided to allow the introduction of compounds at any time during the experiment. In addition, there are three sets of openings for connections.
Each side of the reservoir has two outlets to which a short piece of tubing connects to the fittings in the vertical ports of the Ussing chamber. This is the buffer circulation connection.
Each side of the reservoir has one inlet for an air/gas connection. Gas, commonly a 95% O2/5% CO2 mixture, is forced under low pressure into these openings and allowed to come into contact with the buffer solution. Bubbles, thus formed, generate a circulation and oxygenate the buffer. This is commonly referred to as a gas-lift perfusion system.
The center of the reservoir contains one inlet and one outlet for water circulation. Water is pumped through the water jackets which surround the buffer circulation system and maintains the perfusion solutions at constant temperature. Using this system, the effect of temperature on the subject epithelium can be studied.

The support stand is manufactured from white acrylic and is mounted on four rubber, no-slide feet. The support stand includes anodized aluminum hardware comprised of the pre-mounted chamber support bracket, two threaded ring stands and one 3-prong utility clamp.

Each chamber is supplied with a a set of 4 electrodes, 2 Ag-AgCl pellet electrodes for voltage and 2 Ag wire electrodes for current. The electrodes include 1 meter (3 feet) of wire and a connector to plug into the amplifier headstage.

Luer taper fittings screw onto the electrodes to provide agar salt bridge connection to the chamber. The fittings are filled with agar either before or after assembly to the electrode, depending on the length and tip size of the fitting.