The UP-100 is a multi purpose perfusion system best utilized when different types of organs must be perfused either in situ or ex vivo. The modular design of this system allows easy adaptation to different applications using additions or extensions to the base unit.
Applications for in situ perfusion of hind limb, hindquarter mesenteric bed, liver, kidney:
• Blood vessel tone in peripheral vascular bed
• Balance tests by muscle work (glucose / lactate / pyruvate, high energy phosphates / orthophosphate, etc.)
• Test of vasodilative drugs in occlusive diseases of legs
• Test of muscle relaxants (end-plate pharmacology)
Applications for ex-vivo perfusion using a moist chamber of liver, kidney, mesenteric bed:
• Test of vasodilative drugs
• Studying metabolic processes
• Neural vascular tone
• Organ preservation for transplant
Applications for perfusion of isolated mouse, rat, guinea-pig or very small rabbit heart
Perfusion according to Langendorff. Constant flow or constant pressure perfusion are available using the same equipment.
• If isolated heart is only used occasionally and basic parameters are needed
• For student lab
Internal organs (kidney, liver, mesenteric bed) must obviously be kept under optimal physiological conditions, moist and at defined temperature during perfusion. This requires a suitable chamber with the necessary tubing connections for cannulating the organ. Chamber and cover of the moist chamber are both jacketed and are thermostated by passing warm water through them. The interior of the chamber measures 105 x 65 x 16 mm. Using a spacer ring the clear height of the interior can be increased to 34 mm. At the bottom of the interior is a removable cork plate; this is used to carry the tissue which is secured with needles inserted in the cork. Several tubes and apertures in the chamber wall are used for cannulating the tissue and for connecting any measuring instruments required (pressure, flow, electrochemical parameters, etc.). The interior has threaded holes at the sides for fixing mini ball clamps (option) (e.g., for supporting connecting cannulae).