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• Easily expandable system available in different configurations - up to 32
physiocages
• Highly sensitive and stable sensors for O2 and CO2
• The monitoring of the food and drink consumption and activity uses the newly
adapted weight transducer technology, providing a higher accuracy and stability
• External food and drink dispensers avoid any influence on the living space of the
subject as well as unwanted artifacts
• Registers the absorbed food & drink and its wastage by the means of specifically
adapted systems
• Rearing can be assessed using an additional Infrared frame
The PHYSIOCAGE system is a modular system allowing the combination of respiratory metabolism (O2 consumption/CO2 production), food & drink intake, activity and rearing measurements in specifically adapted home cages in rodents. The major feature of the PHYSIOCAGE system is that it is easily expandable to any of the options of the system.
Respiratory metabolism is evaluated by means of indirect calorimetry using the components of the Panlab/Harvard Apparatus OXYLET system which has been particularly optimized for studies on laboratory rodents (mice and rats). The system enables the monitoring of carbon dioxide (CO2) production and oxygen (O2) consumption as well as the calculation of related physiological parameters (respiratory quotient, energy exchangeÉ). An Air Flow and Switching Unit provides a constant air flow which can be adjusted for each experimental chamber. It also organizes the cycles of sequential gas measurements between the different chambers. The Gas Analyzer unit measures the concentration of oxygen (O2) and carbon dioxide (CO2) for every experimental chamber sequentially and directly send the data to the METABOLISM Software (META-OXY module) for analysis.
Food & drink intake and activity are evaluated using the weight transducer technology developed in the Panlab/Harvard Apparatus FooDrink system. This technology allows the continuous assessment of the animal food and drink consumption and spontaneous activity with highest accuracy and stability of the measurements. The food, drink and activity signals from the weight transducers are amplified using the Panlab/Harvard Apparatus LE520 and LE530 Amplifiers and directly send to the METABOLISM Software (META-INT and META-ACT modules) for analysis.
Rearing assessment need an additional Infrared (IR) frame connected to the PHYSIOCAGE system. The rearing signal obtained from the infrared beam cuts is amplified using the Panlab/Harvard Apparatus LE530 Amplifier.
Note that METABOLISM software integrates the three possible specific software modules: META-OXY for calorimetry, META-INT for FooDrink and META-ACT for activity and rearing.
Optionally, the system can be connected to a Data Acquisition System (PowerLab® recommended) for visualizing data in analogical format.
Citations
Clerc P et al. (2007) Involvement of Cholecystokinin 2 Receptor in Food Intake Regulation: Hyperphagia and Increased Fat Deposition in Cholecystokinin 2 Receptor-Deficient Mice. Endocrinology 48(3): 1039-1049. (mice, France)
Bauche IB et al.(2006) Overexpression of Adiponectin Targeted to Adipose Tissue in Transgenic Mice: Impaired Adipocyte Differentiation. Endocrinology 148(4):1539-1549. (Energy expenditure, France)
Francklauser S et al. (2006) Adipose Overexpression of Phosphoenolpyruvate carboxykinase leads to high susceptibility to diet-induced insulin resistance and obesity. Diabetes 55:273-280. (Oxymetry, food/drink, Activity. Mice, Spain)
Bienvenu G et al. (2005) Dysregulation of energy homeostasis in mice overexpressing insulin-like growth factor-binding protein 6 in the brain. Diabetologia. 48(6): 1189-1197. (O2 consumption, mice, France)
Prieto-Lloret J et al. (2003) Ventilatory responses and carotid body function in adult rats perinatally exposed to hyperoxia. J. Physiol. 154: 126-144 (LE 400-4, spirometry, rat, Spain)
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