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Microinjection Systems
Picoliter Injector Microinjection Systems
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Picoliter Injector Microinjection Systems
The PLI-90 Pico-Injector, like the reliable PLI-100 Pico-Injector, controls the precise and reporducible regulation of injection pressure and time. It is for applications that do not require a vacuum for filling pipettes barrels or if using a holding pipette for holding cells is not essential to your application.
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• 3 pressures — inject, balance, and clearing • Eliminates backflow into pipette after injection • Reproducibly delivers femtoliters to microliters • Easy to use • Economical • Precise
The PLI-90 Pico-Injector reliably delivers a wide range of volumes through micropipettes by applying a regulated pressure for a digitally set period of time. Compressed gas allows the user to deliver desired volumes from femtoliters to microliters while simultaneously holding a cell. Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-90 is well suited for your experiment. Easy to Use Inject with the touch of a button or a tap of the foot switch — it’s as easy as that! Reproducible Pressure to the pipette is controlled precisely through a multi-turn regulator, and is reported digitally for easy repeat. Injection time is digitally controlled in 10 msec steps between 0.01 to 0.99 seconds (and in 1 second steps between 1 to 99 seconds). Pipette tip diameter, and hence delivered volume, are easy determinations once the timing and pressure are known. Versatile Deliver volumes from femtoliters to microliters with the same instrument, resulting in a wide range of applications. Pressure Capabilities The PLI-90 features two negative and three positive pressure pneumatic capabilities. The negative, or vacuum functions allow the user to: 1. Fill micropipettes from their tips, reducing wastage of valuable injectables. 2. Provide a means to secure and manipulate a cell using a holding pipette. The positive pressures allow the user to: 1. Eject precise amounts of fluids. Selection Guidelines The PLI-90 is available in two packages: Basic and Plus. The basic unit is supplied with an input and output hose, handle, power cord and instruction manual. The plus unit is supplied with all of the same parts as the basic unit but also includes a foot switch, pipette holder and input hose adapter.
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