The LU-CB1 Leiden Micro-Incubator provides stable culture conditions (temperature, pH, aseptic isolation, and osmolarity) during continuous or intermittent observations when media temperatures between ambient and 50°C are desired.
The LU-CBl is used primarily for static (no perfusion) chamber media applications. It features excellent mechanical and optical access to cells or tissue. Measuring probes (temperature, pH, etc.), recording electrodes, and micropipettes can easily be manipulated in and out of the chamber.
Aseptic isolation of the culture media can be provided by using a layer of non-toxic mineral oil. A gas flow is directed over the oil to control the micro-environment (including pH) and to minimize temperature gradients across the chamber. It is possible to use the LU-CB1 as a perfused system by preheating the perfusate close to the desired temperature before it enters the chamber.