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• Provides two identical streams of any mix of O2 and CO2 gas
• Full range real-time control of both gases
• Simultaneous control of both gas and liquid phases, useful for live cell microscopy
• Easily switch from one gas concentration to another
• Eliminates the inflexibility and high cost of premixed gas, and inaccuracies due to gas stratification in premixed gas tanks
• Vertical footprint saves bench space
The Oxystreamer® has two output streams can be used to simultaneously condition the atmosphere in a microscope stage mount cell culture incubators/chambers and the dissolved oxygen and carbon dioxide levels in media or perfusate. The following is a list of the major benefits derived from replacing pre-mixed gas cylinders:
1. Precise, variable, selection of oxygen and carbon dioxide gas concentrations (ratios)
2. Substantial cost savings by using standard, low cost cylinders of oxygen, carbon dioxide and nitrogen gas
3. Eliminates the stratification induced concentration errors that are inherence in premixed gas cylinders
4. By independently monitoring and controlling oxygen and carbon dioxide concentrations of the streams, the OxyStreamer® can monitor and proportionally mix (blend) oxygen, carbon dioxide and nitrogen gases into any concentration combination in real time.
The OxyStreamer® models are easy to use. After calibrating the oxygen and carbon dioxide sensors, the desired concentrations are simply entered into their respective controllers using the digital display and up/down control buttons. The closed-loop dynamic gas control system will then automatically monitor and adjust the output stream of gas to the pre-programmed gas ratios in the controllers. This technique will maintain the output mixed gas concentration to within 0.2% of the selected set-point levels.
The flow rate for each stream is independently controlled for gas efficiency and to provide a method for setting a reproducible rate at which the gas is delivered to the cell culture chamber and/or diffused into the cell culture media or perfusate. The inlet ports for the gases on the back of the unit are 1/4 inch hose barb fittings and will take standard 1/4 inch ID tubing. Output stream ports are quick-disconnect fittings that use 1/16 inch or 1/8 inch ID tubing.
Options include a Windows® based software package that provides trend plotting, data logging, and remote operation via RS-232
connection to your PC. Multiple Oxystreamer® models can be daisy chained via optional RS-485 interface.
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