Very Large Rectangular Open Bath for Slice Studies (RC-29)
The RC-29 is designed for slice studies requiring controlled atmospheric conditions.
- Designed for tissue slice studies
- Gas perfusion ports allow for controlled atmospheric conditions
- Provides for perfusate flow both above and below a tissue slice
- Provides good access for immersion optics and electrodes
- Can be used with either upright or inverted microscopes
- Large imaging area
- Slice anchors available
The RC-29 chamber is modeled after the RC-27L (in that it enables solution flow both above and below the tissue slice) with the added feature of atmospheric environmental control.
Similar to the RC-27L, the tissue slice rests on a plastic slice support 0.5 mm above the coverslip floor and is held in place by an optional slice anchor. Slice supports and slice anchors use fine Lycra threads to secure the slice into place allowing maximum access to the preparation. Thread spacing is 1 mm on the slice support and are 1.0, 1.5, and 2.0 mm for the slice anchors. Anchors are purchased separately and are available in polycarbonate and stainless steel. Warner also offers anchor kits containing slice anchors (1 each) in each available thread spacing.
The RC-29 has tall sides with sloping surfaces to allow for an atmospheric space. Gas input ports are constructed in the chamber walls and a chamber top, with access port, is also provided. Continuous gas flow into the chamber establishes positive pressure within the chamber maintaining the atmospheric environment.
Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.
The RC-29 chamber is supplied with a plastic SS-3 slice support, 10 ft of PE-160 polyethylene tubing, a chamber cover, and one pack of #1 thickness coverslips (CS-24/50, 24 x 50 mm, pkg. of 50).
(L x W x H)
|38 x 18 x 6 mm|
(L x W x H)
|50 x 25 x 4.5 - 8 mm|
|Volume, by depth||629 μl/mm|
|Bottom coverglass||24 x 50 mm|
|Slice support||24 x 50 mm, polycarbonate
Lycra threads at 1 mm
(ID x OD)
|1.14 x 1.57 mm (PE-160)|
|Aspirator coupling||1.67 mm (OD)|
|Gas perfusion ports||Stainless steel, 1.67 mm (OD)|
|Integrated Components Brochure_06_7_11.pdf||Integrated Components for Live Cell Imaging Brochure|
|RC-27L RC-29 Wicking (2000.05.15).pdf||Information for RC27L and RC-29 Wicking Chambers|
|RC-29 (2000.09.02).pdf||RC-29 Chamber Instructions|
|perfusion_strategies.pdf||Perfusion Strategies (Leinders-Zufall)|