Ultra-quiet Imaging Chambers for Slice Studies (RC-27D and RC-27LD)

Ultra-quiet Imaging Chambers for Slice Studies (RC-27D and RC-27LD)

The RC-27D and RC-27LD are designed for applications requiring ultra quiet solution delivery.

  • Designed for tissue slice studies
  • RC-27LD includes slice support
  • Eliminates vibration associated with solution delivery
  • Alows multiplexed solution exchange without switching artifacts

Item# Description U.S. List Price Quantity
64-1548 Ultra Quiet Imaging Chamber, large bath, IV drip type
64-1532 Ultra Quiet Imaging Chamber, very large bath, IV drip type
64-1529 PM-7 Chamber platform with magnetic clamps
64-1530 PM-7D Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0710 CS-24/60 Coverslips for use with RC-10, RC-11, RC-13, and RC-16, 0.22 mm (0.0086 in), 24 x 60 mm, pkg of 40
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-0256 Slice Anchor - Stainless Steel for RC-27L & RC-29, 1.0 mm, SHD-27LH/10
64-0257 Slice Anchor - Stainless Steel for RC-27L & RC-29, 1.5 mm, SHD-27LH/15
64-0258 Slice Anchor - Stainless Steel for RC-27L & RC-29, 2.0 mm, SHD-27LH/2
64-0259 Slice Anchor - Plastic for RC-27L & RC-29, 1.0 mm, SHD-27LP/10
64-0260 Slice Anchor - Plastic for RC-27L & RC-29, 1.5 mm, SHD-27LP/15
64-0261 Slice Anchor - Plastic for RC-27L & RC-29, 2.0 mm, SHD-27LP/2
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The large imaging field in the RC-27D and RC-27LD allows for maximal observation of biological specimens with the addition of a sloped input. Advantages of this system over other chambers includes the presence of a sloped inflow channel which allows for smooth and ultra-quiet solution delivery.

The sloped input allows I-V type infusion lines to deliver drops at a fixed rate without the transfer of noise to the specimen field. In addition, multiplexed solution delivery is now possible without the potential for solution switching artifacts. This technology provides the capability of ultra-quiet solution exchange without transferring noise to either the system or to the image.

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Ultra-quiet solution delivery while measuring from tissue slices is provided for by the inclusion of a slice support in the RC-27LD chamber. Slices are supported 0.5 mm above the chamber floor. Support threads are made of Lycra and thread spacing 0.5 mm.

The RC-27D requires a PM-7 platform while the RC-27LD requires a PM-7D platform. Both chambers also require a Series 20 stage adapter to complete the assembly for mounting onto a microscope.

Each chamber is supplied with a MAG-2 magnetic clamp for suction tubes, a magnetic perfusion tube holder, a tissue slice support, a package of #2 thickness glass coverslips (CS-24/60), 40/pkg, and 10 feet of PE-160 polyethylene tubing. The RC-27LD also includes a slice support. Slice anchors are sold separately.

Specifications
Material Polycarbonate
Bath dimensions
(L x W x H)
38 mm x 18 mm x 2.5 mm
Outer dimensions
(L x W x H)
59.9 mm x 25.4 mm x 4.2 mm
Volume, by depth  
Bottom coverglass 24 x 60 mm


RC-27D Drawing

  1. Cholecystokinin Activates Orexin/Hypocretin Neurons through the Cholecystokinin A Receptor
    Tsujino N, Yamanaka A, Ichiki K, Muraki Y, Kilduff TS, Yagami K, Takahashi S, Goto K, Sakurai T
    J Neurosci. 2005 Aug 10;25(32):7459-69
  2. Chronic Cocaine Administration Switches Corticotropin-Releasing Factor2 Receptor-Mediated Depression to Facilitation of Glutamatergic Transmission in the Lateral Septum
    Liu J, Yu B, Orozco-Cabal L, Grigoriadis DE, Rivier J, Vale WW, Shinnick-Gallagher P, Gallagher JP
    J Neurosci. 2005 Jan 19;25(3):577-83
DOCUMENT DESCRIPTION
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-27LD (080724).pdfRC-27LD Chamger Manual
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)