Two Electrode Voltage Clamp Workstation (TEV-700)

Two Electrode Voltage Clamp Workstation (TEV-700)

This workstation provides many of the necessary components typically used with the OC-725C voltage clamp for research involving Oocytes.

Item# Description U.S. List Price Quantity
64-0034 TEV700 Complete Oocyte Two-Electrode Voltage Clamp Work Station
64-1007 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.0 mm Glass ID
64-1008 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.2 mm Glass ID
64-1009 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.5 mm Glass ID
64-1010 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 2.0 mm Glass ID
64-1051 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.0 mm Glass OD
64-1052 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.2 mm Glass OD
64-1053 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.5 mm Glass OD
64-1054 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 2.0 mm Glass OD
64-0185 Model VC-8P Valve Control System/8 pinch valves
64-0135 Model VC-66CS Complete Pinch Valve System with 6 Valves
64-1940 (DWV) Dedicated Workstation Vacuum
69-0130 Nanoject II Nanoliter Injector, 115 V, 60 Hz, US Plug
64-1586 (BPM-1) Base plate, magnetic steel, 12x24 x 3/8', thick powder coating"
72-0311 NCL 150, 120V/60Hz
72-0267 Bifurcated Gooseneck Light Guide, Black, 4.7 mm fiber diameter (each arm)
64-1803 Binocular Zoom Stereo Microscope, 0.8-5.0x zoom, with Boom Stand
64-0028 OC-725C Oocyte Clamp Supplied with 7250V voltage headstage, 7251I bath clamp headstage, 7259C current cable, model membrane and rack mount hardware. Specify line operating voltage if other than 100-130 VAC. One straight holder and one 45° holder nee
64-0319 RC-3Z Oocyte Chamber
64-0055 Standard Manual Control Micromanipulator With One Holder Left-Handed Clamp
64-0056 Standard Manual Control Micromanipulator With One Holder Right-Handed Clamp
64-0060 MB/B Magnetic Base
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The TEV-700 Workstation provides many of the necessary components typically used with the OC-725C voltage clamp for research involving Oocytes. When these components are purchased with the OC-725 Oocyte Clamp, the complete package is discounted, as shown below in the order information section.

BPM-1 - This solid steel plate provides a stable platform for mounting micromanipulators mounted on magnetic bases. A highly durable powder coating applied to the surfaces makes it impervious to most common lab spills.

Electrode Holders

Two vented electrode holders with silver wires are typically required with the clamp; a straight type for use with the voltage headstage and a 45° type with mounting handle for use with current electrodes. Vents have been added to the electrodes to prevent pressure build-up inside the electrode which can damage oocytes.

Select the holder based on your glass outer diameter. Unvented holders are available and have a 'N' at the end of the model number. Vented holders have a 'V' on the end of the model number. A two meter length cable assembly is provided to connect the current electrode holder to the clamp.

Holders are available separately.

System includes:

  • Oocyte Clamp Amplifier; OC-725C; 64-0028
  • Oocyte Recording Chamber; RC-3Z; 64-0319
  • Left Micro-Manipulator; MM-33L; 64-0055
  • Right Micro-Manipulator; MM-33R; 64-0056
  • Magnetic Base (2 in complete system); MB/B; 64-0060
  • Voltage and current electrode holders must be purchased separately

Optional magnetic steel base plate available; BPM-1; 64-1586

Specifications
OC-725C Oocyte Clamp Specifications
Test Condition #1 Model cell used to obtain specifications. Model membrane: 1 MΩ in parallel with 220 nF. Current and voltage electrodes both 1 MΩ impedence.
Test Condition #2 Noise measurements made with an 8-pole Bessel filter.
Voltage Recording Channel (Vm) V Probe Input Impedance 0.5 x 1012 &, 1 pF
Output Resistance 100 Ω
DC Offset ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn).
Noise (0-10 kHz) 3 µV RMS with input grounded. 20 µV RMS with model cell.
Electrode Test 10 mV/MΩ read on meter 100 mV/MΩ at Vm x10 output.
Meter Range ± 199.9 mV full scale
Capacity Compen 0 - 90 pF
Current Sensing Channel (Bath Clamp) (Im)

Noise (0-1 kHz) 4.5 nA RMS with bath clamp
10 nA RMS in output leg
I Monitor Output 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10.
Gain Telegraph Output 0.2 to 2.6 V in 7 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software*
Meter Range, full scale Clamp Current: ± 199.9 µA
Electrode Voltage Ve ± 199.9 mV; current meter reads Ve when clamp mode switch is off.
Current Electrode Channel Compliance Voltage ± 180 V
Clamp Speed 350 µsec; (10-90%) with 100 mV square wave command applied to model cell.
Gain Variable AC/DC: 0 to 2000
Fixed DC Gain Switch selected: 1 x 106
Ve DC Offset ± 200 mV at input (20 mV/turn)
Electrode Test 10 mV/MΩ read on current meter.
100 mV/MΩ at Ve x10 output rear panel
Commands Hold Manually set with digital potentiometer, 2 digit resolution and 2 ranges:
x1 range: ± 1 to 99 mV in 1 mV steps
x2 range: ± 2 to 198 mV in 2 mV steps
External Signals applied to COMMAND IN÷10 1 V applied = 100 mV
Physical Dimensions Case 8.9 cmx 43.2 cmx 30.5 cm
Voltage Headstage 12.5 mm D x 5 cm L with 1.8 m cable
Mounting Handle 4.8 mm D x 6.3 cm L
Bath Headstage 2.3 cm x 3.5 cm x 4.2 cm with 1.8 m cable
Shipping Weight 6.8 kg
Warranty Three years, parts & labor
Power Requirements 100-130 V or 220-240 VAC, 50/60 Hz, 20 VA
RC-3Z Oocyte Recording Chamber Specifications
Material Polycarbonate
Footprint, L x W 13 x 7.7 cm (5.1 x 3 in)
Bath Dimensions
Narrow Bath 27 x 3.2 x 3.2 mm
Volume ~85 µl/mm Height
Wide Bath 27 x 6.3 x 3.2 mm
Volume ~170 µl/mm Height
Chamber Floor Insert 25 x 75 x 1 mm Polycarbonate
Input Tubing Size 1/16 in OD (PE-160)
Output Tubing Size 1/16 in ID
  1. On-Demand Webinar: Data Acquisition and Recording with TEV-700 Oocyte Workstation
    Warner Instruments
    Vimeo 2013
References
  1. Maprotiline Block of the Human Ether-a-go-go-related Gene (HERG) K+ Channel
    Su-Hyun Jo, Hee-Kyung Hong, Sung Jun Jung, Seon Ha Chong1, Ji-Hyun Yun, Young-Sang Koh, and Han Choe1
    Arch Pharm Res Vol 30, No 4, 453-460, 2007
  2. A Simple, Cross-linked Collagen Tissue Substitute for Corneal Implantation
    Liu Y, Gan L, Carlsson DJ, Fagerholm P, Lagali N, Watsky MA, Munger R, Hodge WG, Priest D, Griffith M
    Invest Ophthalmol Vis Sci. 2006 May;47(5):1869-75
  3. The cholinergic antagonist bungarotoxin also binds and blocks a subset of GABA receptors
    McCann CM, Bracamontes J, Steinbach JH, Sanes JR
    Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5149-54
  4. The anxioselective agent 7-(2-chloropyridin-4-yl)pyrazolo-[1,5-a]-pyrimidin-3-yl](pyridin-2-yl)methanone (DOV 51892) is more efficacious than diazepam at enhancing GABA-gated currents at alpha1 subunit-containing GABAA receptors
    Piotr Popik, Emmanuel Kostakis, Martyna Krawczyk, Gabriel Nowak, Bernadeta Szewczyk, Philip Krieter, Zhengming Chen, Shelley J. Russek, Terrell T. Gibbs, David H. Farb, Phil Skolnick, Arnold S. Lippa, and Anthony S. Basile
    JPET Fast Forward. Published on September 13, 2006
  5. PMA- and ANG II-induced PKC regulation of the renal Na -HCO3 cotransporter (hkNBCe1)
    Clint Perry, Judith Blaine, Hong Le and Irina I. Grichtchenko
    Am J Physiol Renal Physiol 290:417-427, 2006
  6. Chemical Modification of the Human Ether-a-go-go-related gene (HERG) K+ Current by the Amino-Group Reagent Trinitrobenzene Sulfonic Acid
    Su-Hyun Jo1, Se-Young Choi, Ji-Hyun Yun4, Young-Sang Koh, Won-Kyung Ho5, and Chin O. Lee
    Arch Pharm Res Vol 29, No 4, 310-317, 2006
  7. Oxidation and Reduction Control of the Inactivation Gating of Torpedo ClC-0 Chloride Channels
    Yong Li, Wei-Ping Yu, Chia-Wei Lin, and Tsung-Yu Chen
    Biophysical Journal Volume 88 June 2005 3936–3945
  8. Inhibition of Human ether-a-go-go-Related Gene K+ Channel and IKr of Guinea Pig Cardiomyocytes by Antipsychotic Drug Trifluoperazine
    Se-Young Choi, Young-Sang Koh, and Su-Hyun Jo
    JPET 313:888-895, 2005
DOCUMENT DESCRIPTION
Connecting the OC-725 to pclamp (2005.06.24).pdfConnecting the OC-725 to pClamp