Steiert Organ Bath System

Steiert Organ Bath System

Designed for simultaneous action potential and contraction force measurements on cardiac tissue preparations such as papillary muscle and Purkinje fibers

To ensure that your system is properly configured to meet your application needs, please contact Technical Services at 800-547-6766 or via email before placing an order. We would also be happy to answer questions you might have about any of our tissue and organ bath systems.

Item# Description U.S. List Price Quantity
73-2152 Steiert Organ Bath, Basic Unit, 230 VAC, 50 Hz
73-2892 Steiert Organ Bath, Basic Unit, 115 VAC, 60 Hz
73-3602 Ag/AgCl Electrode for Steiert Organ Bath
73-3087 Platin Field Electrode for Mayflower Tissue Bath and Steiert Tissue Bath, Set of 2
73-3603 Magnetic Base for DC-3K to Steiert Bath
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The Steiert Tissue bath system is a horizontal bath, open at the top, specifically designed for studies of cardiac tissue preparations such as papillary muscle and Purkinje fibers. The unique design allows the user to record both intracellular action potentials and contractile force from the same tissue preparation.

Features & Benefits

  • Compact and easy-to-use setup
  • Suitable for papillary muscle and Purkinje fibers
  • Mini coaxial electrode for electrical stimulation

Applications

  • Electrophysiology (Intracellular Action Potential) studies on heart muscle
  • Mechanical (Contractile Force) studies on heart muscle
  • Simultaneous action potential and contraction force measurements
  • Recording of intracellular action potential and force development 


Included items (Core System): Tissue bath, DC controller (for temperature regulation and perfusate warming), REGLO analog peristaltic pump, direct contact coaxial stimulation electrode with connecting cable and force transducer mounting rod with micrometer control to maintain tissue tension


Set Up and Operation:

The tissue bath has ports for fluid inlet and outlet. The low volume bath design features an adjustable height sipper tube to adjust and set the bath volume (1 to 2 ml). The inner dimension of the oval bath are 15 mm wide x 20 mm oblong. The port outlet connects to the peristatic pump.

The isometric force transducer is mounted with micrometer control to maintain and monitor tissue tension and is in direct contact  with the coaxial stimulation electrode. Silicone grease is used to seal the opening through which the tissue support extends from the tissue preparation to the force transducer.

The DC Temperature Controller has been specially developed for thermostating the perfusion solution supply on the Steiert organ bath. Special attention has been paid during the development of this controller to a very low radiated interference level. The heating circuit (temperature controller and heating element) has excellent decoupling from the perfusion solution circuit. The heating element is a long life halogen bulb. It is therefore excellently suited for the interference-free measurement of intracellular action potentials.

The tissue under investigation is secured with needles to a silicone pad fitted in the bath bottom, the other end is secured, either with a thread or directly to the hook of the force transducer.

Stimulation takes place through a miniature coaxial electrode which is mounted flexibly on a triple ball joint to the back of the bath. The stimulation electrode can therefore be positioned to suit individual requirements.


Additional equipment required (fully functional system): F30 Force Transducer, bridge amplifier for the force transducer and PLUGSYS Microelectrode Amplifier Module* with matching headstage amplifier, data acquisition, recording microelectrodes, electrical stimulator with isolation unit, tissue preparation dish, anti-vibration table, microscopes, illumination, micromanipulators and other options based on specific application.

*The Microelectrode Amplifier Module (MEA) includes a separate headstage amplifier used to record intracellular potentials. The headstage amplifier is easily mounted on a micromanipulator and the open bath design facilitates electrode placement.

Note: If contractile force measurement is not required, special versions are available on request. A version with field stimulation is also available on request. Be aware that in this case stimulation artifacts may interfere with the action potential signal.

Specifications