Slotted Bath with Field Stimulation (RC-21BRFS)

Slotted Bath with Field Stimulation (RC-21BRFS)

Modeled on the RC-21 Series chambers, RC-21BRFS imaging chamber is designed for field stimulation studies.

  • Designed for studies involving cardiac myocytes
  • Field stimulation chamber
  • Closed bath design
  • Small volume with slotted bath
  • Ideal for fluorescent, calcium and time-lapse imaging studies

Item# Description U.S. List Price Quantity
64-0226 Imaging Chamber RC-21BRFS, with Field Stimulation
64-0278 Series 20 Plain Platform, Model P-2
64-0285 Series 20 Heater Platform, Model PH-2
64-1561 PM-2 Chamber platform with magnetic clamps
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0705 CS-25R Coverslips, 0.15 mm (0.006 in), 25 mm diameter, pkg of 100
64-1424 SIU-102 Stimulus Isolation Unit
64-1425 CC-102 Cable, male banana to 1 mm jacks (for use with Warner field stimulation chambers)
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
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The RC-21BRFS is a small volume, closed bath imaging chamber designed for field stimulation studies. It uses a pair of 25 mm round coverslips to form the top and bottom of the chamber, and the total bath volume is 263 µL. Two platinum wire electrodes are attached to the parallel sides of the oval-shaped bath. The connecting wires are 15 cm long and are terminated in 1 mm pins.

An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe*. It may be used to inject substances into the flow path or to remove air bubbles from the sealed chamber. At the bath output, a length of tubing is used as a back pressure control to insure smooth perfusate flow.

RC-21BRFS chambers require a platform (P-2, PH-2, or PM-2) and stage adapter to complete the assembly for mounting onto a microscope.

Available Field Stimulator

The SIU-102 is a bipolar stimulator designed for use with Warner field stimulation chambers. This instrument features constant current and constant voltage modes, as well as bipolar, pulse and DC modes. Optical coupling is used to electrically isolate the stimulator from the pulse source. Currents up to 100 mA and voltages up to 100 V are supported.

Each RC-21BRFS chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 polyethylene tubing.

* 25 Gauge (0.45mm OD) needle

Specifications
Material Polycarbonate
Bath dimensions
(L x W x H)
18.0 x 6.3 x 2.3 mm
Outer dimensions
(L x W x H)
47.5 x 28.5 x 2.5 mm
Volume 263 μl
Electrodes Platinum wire
15 cm leads with 1 mm pins
Top coverglass 25 mm round
Bottom coverglass 25 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)

RC-21BRFS drawing

  1. The Phospho-Dependent Dynamin–Syndapin Interaction Triggers Activity-Dependent Bulk Endocytosis of Synaptic Vesicles
    Clayton EL, Anggono V, Smillie KJ, Chau N, Robinson PJ, Cousin MA
    J Neurosci. 2009 Jun 17;29(24):7706-17
  2. Superfluous Role of Mammalian Septins 3 and 5 in Neuronal Development and Synaptic Transmission
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    Mol Cell Biol. 2008 Dec;28(23):7012-29. Epub 2008 Sep 22
  3. Two-Photon Fluorescent Probes for Biomembrane Imaging: Effect of Chain Length
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    Chembiochem. 2008 Nov 24;9(17):2830-8
  4. Differential labelling of bulk endocytosis in nerve terminals by FM dyes
    Clayton EL, Cousin MA
    Neurochem Int. 2008 Sep;53(3-4):51-5. Epub 2008 Jun 11
  5. Myocardial injection of skeletal myoblasts impairs contractility of host cardiomyocytes
    Giraud MN, Liechti EF, Tchantchaleishvili V, Siepe M, Cook S, Carrel TP, Tevaearai HT
    Int J Cardiol. 2008 Sep 20
  6. Bulk Synaptic Vesicle Endocytosis Is Rapidly Triggered during Strong Stimulation
    Clayton EL, Evans GJ, Cousin MA
    J Neurosci. 2008 Jun 25;28(26):6627-32
  7. Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
    Anggono V, Cousin MA, Robinson PJ
    Methods Mol Biol. 2008;457:333-45
  8. Physiological Activity Depresses Synaptic Function through an Effect on Vesicle Priming
    Krista L. Moulder, Xiaoping Jiang, Amanda A. Taylor, John W. Olney, and Steven Mennerick
    The Journal of Neuroscience, June 14, 2006, 26(24):6618–6626
  9. Studying vesicle cycling in presynaptic terminals using the genetically encoded probe synaptopHluorin
    Burrone J, Li Z, Murthy VN
    Nat Protoc. 2006;1(6):2970-8
  10. Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
    Gandhi SP, Stevens CF
    Nature. 2003 Jun 5;423(6940):607-13
DOCUMENT DESCRIPTION
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-21BRFS (080724).pdfRC-21BRFS Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)