Self-Contained Ussing Chambers (U9926, U2500)

Self-Contained Ussing Chambers (U9926, U2500)

The U9926 and U2500 Self-Contained Ussing chambers improve on the Classic design by bringing the solution reservoir into the chamber housing. This dramatically reduces the size of the apparatus and increases the ease-of-use of the system.

  • Modular design for separate inserts
  • No glass circulation reservoirs
  • Inserts are quickly exchanged between experiments
  • Solution warming is provided by pumping heated water through the chamber halves. 

 

How to order (Please see Item Listing):
1. Select Base Assembly
Each system includes one or two U9975A Electrode Set(s) and U9965EP Electrode Bridge Fitting Kit(s)
2. Select Insert
Order 1 if using the U9926 and order 2 if using the U2500

Please see the Full Description for complete information about Self-Contained Ussing Chambers.

 

 

Item# Description U.S. List Price Quantity
64-0568S U9926/A Single Channel Self-Contained Ussing Chamber with (A) O-Ring Insert
64-0575S U2500 Dual Channel Self-Contained Ussing Chamber
64-0582 3.8 mm Round Insert with O-Rings for U9926 and U2500 Self-Contained Ussing Systems
64-0583 6.0 mm Round Insert with O-Ring for U9926 and U2500 Self-Contained Ussing Systems
64-0584 9.0 mm Round Insert with O-Ring for U9926 and U2500 Self-Contained Ussing Systems
64-0585 12.0 mm Round Insert with O-Ring for U9926 and U2500 Self-Contained Ussing Systems
64-0586 13.5 mm Round Insert with O-Ring for U9926 and U2500 Self-Contained Ussing Systems
64-0587 3.8 mm Round Insert with 5 Pins for U9926 and U2500 Self-Contained Ussing Systems
64-0588 6.0 mm Round Insert with 5 Pins for U9926 and U2500 Self-Contained Ussing Systems
64-0589 9.0 mm Round Insert with 5 Pins for U9926 and U2500 Self-Contained Ussing Systems
64-0590 12.0 mm Round Insert with 5 Pins for U9926 and U2500 Self-Contained Ussing Systems
64-0591 13.5 mm Round Insert with 5 Pins for U9926 and U2500 Self-Contained Ussing Systems
64-0592 Slotted Insert, 1.6 x 7.0 mm, with 6 Pins for use with U9926 and U2500 Self-Contained Ussing Chambers
64-0593 Slotted Insert, 2.4 x 7.0 mm, with 6 Pins for use with U9926 and U2500 Self-Contained Ussing Chambers
64-0594 Slotted Insert, 3.5 x 7.0 mm, with 6 Pins for use with U9926 and U2500 Self-Contained Ussing Chambers
64-0600 Snapwell™ Adapter for U9926 and U2500 Self-Contained Ussing Systems
64-0595 Falcon® Adapter, 9 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0596 Falcon® Adapter, 12 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0597 Falcon® Adapter, 25 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0598 Millicell® Adapter, 12 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0599 Millicell® Adapter, 30 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0601 Transwell® Adapter, 6.5 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0602 Transwell® Adapter, 12 mm for U9926 and U2500 Self-Contained Ussing Systems
64-0565 Tubing/Fitting Kit Includes 3/16in x 10ft (IDxL) Tygon Tubing, Eighteen 1/16in Fittings (six each of straight, Y, and Luer)
64-1521 (U9565SC) Electrode bridge fitting kit, 12 pack
64-0554 Electrode Set, 2 Ag/AgCl Pellet, 2 Ag Wire
64-0555 Bridge Fitting Adapters, pkg of 6
64-0604 (U9605) O-rings for U9926 and U2500 Ussing chambers, 4 pack
72-4924 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz
72-4921 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz
73-3456 Tube Set for Jacketed Buffer Reservoir with Fluid Line Shutoff Valves
98-3402 Sims irrigation nozzle 1/8-1/4, pkg of 25 for use wtih GD120-P5"
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The Self-Contained Ussing Chamber Systems

The Self-Contained Ussing Chambers systems come in single- and dual-channel models and offer many advantages over the Classic design. The single-channel U9926 and dual-channel U2500 are shown to the right. Both systems make use of inserts to secure and position the tissue or culture cup within the chamber body.

Chamber bodies (single- and dual-chamber models) consist of chamber-halves which clamp around the insert(s). Each chamber-half has four ports that enter into its respective bath compartment: a voltage electrode port, a current electrode port, an air/gas port, and a solution drain port. Solution warming is provided by pumping heated water through each chamber-half and there are no delicate glass heat exchangers to be broken. The dual-chamber U2500 has the additional feature of including four needle valves for adjusting the air/gas mixture into each individual chamber-half.

Inserts can be easily exchanged between experiments. Solutions are removed from the chambers through the drain valves and the halves unclamped from each other. The insert is then simply replaced. Inserts are available to support a variety of tissue sizes and culture cups.

Standard Accessories Supplied with U9926 and U2500

The U9926 (single-channel chamber is supplied with 1 electrode set (consisting of 2 voltage and 2 current electrodes), 12 bridge fittings with adapters; and a tubing/ fitting kit. The U2500 (dual channel) chamber is supplied with two each of the above described accessories. Chamber inserts are ordered separately.

Specifications

Each U9926 and U2500 system is supplied with one tissue or culture cup insert. A model letter suffix identifies the insert type.

Insert and culture cup types:

  • (A) Round with O-Ring
  • (B) Round with Sharp Pins
  • (C) Slotted with Pins
  • (F) Falcon
  • (M) Millicell
  • (S) Snapwell
  • (T) Transwell.

Each of the above insert types are supplied in various sizes. Consult the table below to select a specific insert and size and include that information with your order.

Type B and C inserts have the pin configurations shown below.

Insert Type Insert Dimensions Insert Model
Type A Round Insert, 3.8 mm, with O-Ring U9924A-04
Round Insert, 6.0 mm, with O-Ring U9924A-06
Round Insert, 9.0 mm, with O-Ring U9924A-09
Round Insert, 12.0 mm, with O-Ring U9924A-12
Round Insert, 13.5 mm, with O-Ring U9924A-13
Type B Round Insert, 3.8 mm, with 5 Pins U9924B-04
Round Insert, 6.0 mm, with 5 Pins U9924B-06
Round Insert, 9.0 mm, with 5 Pins U9924B-09
Round Insert, 12.0 mm, with 5 Pins U9924B-12
Round Insert, 13.5 mm, with 5 Pins U9924B-13
 Type C Slotted Insert, 1.6 x 7.0 mm, with 6 Pins U9924C-03
Slotted Insert, 2.4 x 7.0 mm, with 6 Pins U9924C-04
Slotted Insert, 3.5 x 7.0 mm, with 6 Pins U9924C-05
Type F Falcon® Adapter, 9 mm U9924F-09
Falcon® Adapter, 12 mm U9924F-12
Falcon® Adapter, 25 mm U9924F-25
Type M Millicell® Adapter, 12 mm U9924M-12
Millicell® Adapter, 30 mm U9924M-30
Type S Snapwell" Adapter, 12mm U9924S
Type T Transwell® Adapter, 6.5 mm U9924T-06
Transwell® Adapter, 12 mm U9924T-12
Transwell® Adapter, 24 mm U9924T-24
  1. Extracellular calcium- and magnesium-mediated regulation of passive calcium transport across Caco-2 monolayers
    Davies SL, Gibbons CE, Steward MC, Ward DT
    Biochim Biophys Acta. 2008 Oct;1778(10):2318-24
DOCUMENT DESCRIPTION
Warner's Guide to Ussing Chamber Systems (091022) WLR.pdfWarner's Guide to Ussing Chamber Systems
U-2500 (1999.07.07).pdfU-2500 User's Manual
U9924M-C12 (2001.03.28).pdfU-9924M-C12 Instructions
U-9926 (1999.11.30).pdfU-9926 User's Manual
Corning-Snapwell-Transwell Instruction Manual1.pdfCorning Guide to Snapwell