Reusable ElectroPrep Dialyzer

Reusable ElectroPrep Dialyzer

The ElectroPrep system from Harvard Apparatus is an extremely versatile patented sample prep technology based on (2D) electrophoresis/dialysis. This ElectroPrep system is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules.

With a run-time of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at the very low currents (5 to 10 mA) used with this system. The sample chambers are made of PTFE, a completely inert material especially suited for high sample recovery. Membranes of different MWCO (molecular weight cut off), from 100 to 300,000 Daltons, can be used for selective elution, filtration, dialysis, fractionation and concentration. Fast DIALYZER can be joined with Link Chamber or Union and membranes.

Item# Description U.S. List Price Quantity
7411-15002D DIALYZER for ElectroPrep, 1500 µl , Qty. of 2
7411-10002D DIALYZER for ElectroPrep, 1000 µl , Qty. of 2
7411-5002D DIALYZER for ElectroPrep, 500 µl , Qty. of 2
7411-2502D DIALYZER for ElectroPrep, 250 µl , Qty. of 2
7411-1002D DIALYZER for ElectroPrep, 100 µl , Qty. of 2
7411-502D DIALYZER for ElectroPrep, 50 µl , Qty. of 2
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Advantages
• Faster dialysis times due to movement of charged molecules in the electric field
• Re-usable
• Available for most sample sizes -large or small
• Membranes available with MWCO's to suit almost any application
• Easy to use
• Leak proof
• Autoclaveable
• Low protein binding
• High sample recovery
• Made of PTFE - totally inert
Applications
• Electro-elution from gels and solutions
• Electro-dialysis (with an average buffer exchange time of 5 to 10 minutes)
• On-line electro-dialysis
• Electro-concentration
• Selective electro-filtration
• Size fractionation
• Primer removal
• Salt removal
• Detergent removal
• Dye-Terminator removal

The ElectroPrep system from Harvard Apparatus is an extremely versatile patented sample prep technology based on (2D) electrophoresis/dialysis. This ElectroPrep system is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules.

With a run-time of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at the very low currents (5 to 10 mA) used with this system. The sample chambers are made of PTFE, a completely inert material especially suited for high sample recovery. Membranes of different MWCO (molecular weight cut off), from 100 to 300,000 Daltons, can band membranes).

Electro-Elution of DNA, Proteins or other Biomolecules from Gel Pieces
Using the ElectroPrep system in the illustrated configuration, elution of DNA, proteins, or any other biomolecules from a gel slice/plug can be achieved quickly and easily with excellent recovery. Chambers can be joined in any combination necessary to accommodate the required gel volume. Samples can be concentrated if desired, by choosing a receiving chamber of suitable volume. The MWCO (molecular weight cut off) of the membranes (a and b) can also be chosen to achieve very selective filtration or size fractionation during the electro-elution process.

Selective Electro-Filtration/Concentration/Separation Based on Different Charges on Biomolecules
In this configuration of the ElectroPrep, the sample is placed in a sample compartment between two membranes (b), both of which should have a MWCO larger than the desired biomolecules. Membranes (a) and (c) should have MWCOs smaller than the biomolecules. Based on their charges, the biomolecules will move to either chamber (1) or chamber (2). Biomolecules with unknown isoelectric points can also be separated and purified using this method.

Electrodialysis through Simultaneous Exchange of Buffers
A sample is placed in the sample compartment between membranes (a) and (b), both of which have MWCOs lower than the molecular weight of the desired biomolecules. The sample is dialyzed through the simultaneous exchange of buffers in the electric field. This method is very fast and very effective. For example, after a PCR reaction, it can be used to rapidly (5 to 10 minutes) remove 100% of the primer. Electrodialysis is also effective for desalting neutral molecules that do not move in an electric field (such as sugars) or charged molecules at their isoelectric point.

Rapid and Selective Electro-Filtration/ Concentration
The sample is placed in the sample compartment comprised of a chamber (1) and the union. The MWCO of membrane (b) should be larger than the molecular weight of the biomolecules and the MWCO of membrane (a) should be smaller. Upon the passage of electric current, the biomolecules will pass through membrane (b) and collect in chamber (2) while smaller molecules will pass through membrane (a). This is a fast and effective method for the concentration of small samples and for selective filtration.
Specifications7411-15002D7411-10002D7411-5002D7411-2502D7411-1002D7411-502D
Filter Mediaµlµlµlµlµlµl
Packing Material1500100050025010050
Tank Depth English333333
Tank Depth English UOMinininininin
Tank Height English444444
Tank Height English UOMinininininin
Tank Width English888888
Tank Width English UOMinininininin