Picoliter Microinjector (PLI-100A)

Picoliter Microinjector (PLI-100A)

The PLI-100A Pico-Injector reliably delivers injections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time.

  • Femtoliter to microliter injections
  • Digital readouts for injection pressure, time, and count
  • Reliable optically encoded circuit for injection time set
  • 5 pressures: inject, balance, clear, fill and hold

Item# Description U.S. List Price Quantity
64-1735 Model PLI-100A Basic Picoinjector with injection, balance, clear, filling and holding pressures. Comes with input hose, output hose, holding hose, and input hose adapter.
64-1736 Model PLI-100A Plus Picoinjector with injection, balance, clear, filling and holding pressures. Comes with input hose, output hose, holding hose, foot switch, pipette holder, and input hose adapter.
64-1737 Model PLI-100A Deluxe Picoinjector with injection, balance, clear, filling and holding pressures, comes with input hose, output hose, holding hose, two foot switches, two pipette holders, and input hose adapter.
64-0060 MB/B Magnetic Base
65-0029 Model PLI-FS Footswitch for Inject, Hold, Fill or Gate (Order more than one if frequent use of footswitch is needed for more than one of these functions.)
65-0006 Model PLI-IHA Input Hose Adapter (1/4 in Male BS4T Pipe Thread) Gas Bottle Regulator Fitting; Connects with PLI-IHO/N
65-0007 Model PLI-IHO Gas Input Hose (Tygon Tubing), 9 ft x 0.250 in (6.35 mm) x 0.125 in (3.18 mm) x 0.062 in (1.57 mm) (L x OD x ID x Wall Thickness); Connects to PLI-IHA and PLI 90/100 Gas Input, Brass Screw Fitting Type - Old
65-0008 Model PLI-IHN Gas Input Hose (Tygon Tubing) 9 ft L x 0.250 in (6.35 mm) OD x 0.125 in (3.18 mm) ID x 0.062 in (1.57 mm) Wall Thickness; Connects to PLI-IHA and PLI 90/100 Gas Input, Quick Connect/Disconnect Type - New
65-0009 Model PLI-OHO Output Hose (Tygon Tubing) 9 ft L x 0.071 in (1.80 mm) OD x 0.039 in (1 mm) ID x 0.016 in (0.41 mm) Wall Thickness; Connects to MSC Pipette Holders, Brass Screw Fitting Type - Old
65-0010 Model PLI-OHN Output Hose (Tygon Tubing) 9 ft L x 0.071 in (1.80 mm) OD x 0.039 in (1 mm) ID x 0.016 in (0.41 mm) Wall Thickness; Connects to MSC Pipette Holders and PLI 90/100 Gas Output, Quick Connect/Disconnect Type - New
65-0011 Model PLI-HHO Holding Hose with Cell Release Bulb; 9 ft L x 0.071 in (1.80 mm) OD x 0.039 in (1 mm) ID x 0.016 in (0.41 mm) Wall Thickness; Connects to MSC Pipette Holders and PLI-100 Hold Output, Brass Screw Fitting Type - Old
65-0012 Model PLI-HHN Holding Hose with Cell Release Bulb; 9 ft L x 0.071 in (1.80 mm) OD x 0.039 in (1 mm) ID x 0.016 in (0.41 mm) Wall Thickness. Connects to MSC Pipette Holders and PLI-100 Hold Output, Quick Connect/Disconnect Type - New
64-1626 (AO161.0) Acrylic pipette holder for 1.0 mm pipettes
64-1627 (AO161.2) Acrylic pipette holder for 1.2 mm pipettes
64-1628 (PLI-PPH) Acrylic Pipette Holder for 1.5 mm pipettes
64-1629 (AO162.0) Acrylic pipette holder for 2.0 mm pipettes
65-0013 Model PLI-PH1 Stainless Steel Pipette Holder, 130 mm L for 1 to 1.5 mm OD Glass Pipettes
65-0014 Model PLI-PH1A Stainless Steel Pipette Holder, 80 mm L for 1 to 1.5 mm OD Glass Pipettes
65-0017 Model PLI-SRG Silicone Rubber Gasket Replacement for use with PLI-PH1 and PLI-PH1A, 10.2 cm (12 in)
65-0015 Model PLI-SRG1.5 Silicone Rubber Gasket Replacement for use with 1.5 mm Glass Pipettes
65-0018 Model PLI-SSB Stainless Steel Bushing Replacement for use with PLI-PH1 and PLI-PH1A Pipette Holders
65-0019 Model PLI-PC Stainless Steel Pipette Cap Replacement for use with PLI-PH1 and PLI-PH1A Pipette Holders
65-0020 Model PLI-HN Stainless Steel Hose Nut Replacement for use with PLI-PH1 and PLI-PH1A Pipette Holders
65-0021 Model PLI-PH-KIT Pipette Holder Hardware Replacement Kit for use with PLI-PH1 and PLI-PH1A Pipette Holders, Includes 1 each PLI-SSB, PLI-PC, PLI-HN and PLI-SRG
65-0022 Model PLI-PHA Stainless Steel Pipette Holder (PLI-PH series) Adapter for Eppendorf ECET FEMTOTIP
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Pressure to the pipette is controlled precisely through a multi-turn regulator, and is reported digitally for reproducibility. Injection time is set using an optically encoded circuit which permits fine and coarse settings from a single knob.

The system timer can be controlled in three ways, front panel push button, foot switch, and external trigger input BNC.

Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-100A is up for the task.

Pressure Capabilities
The PLI-100A features two negative and three positive pressure pneumatic capabilities.
The negative or vacuum functions allow the user to:

  1. Fill micropipettes from their tips, reducing waste of pipettes.
  2. Provide a means to secure and manipulate a cell using holding pipette.

The positive pressures allow the user to:

  1. Inject precise amounts of fluids
  2. Create a balance pressure which prevents backflow into the micropipette following an injection
  3. Clear a micropipette of material in it

Unique Features
The PLI-100A's important 'Balance', 'Hold' and 'Clear' functions are features not found on other microinjection units.

Balance
In addition to the injection pressure, the Pico-Injector offers a secondary balance pressure. This adjustable pressure keeps a positive pressure on the injection pipette before and after injections. This eliminates dilution caused by capillary action and aids in the prevention of clogging. Wasteful continuous injection, which often occurs when no separate balance pressure is offered by an injection device, is avoided.

Fill/Hold
There are two built-in vacuum generators to fill a micropipette from the tip and to hold suspended cells. Filling the pipette from the tip is easier than back filling. The PLI-100A features a Fill Vacuum adjustment on the front panel. Suspended cells can be held with a second (holding) pipette. The holding vacuum’s range accommodates most cell types.

Clear
A high-pressure pulse can be used for clearing a pipette, should it happen to clog. This is particularly useful when working with pipettes sized for smaller volumes.

Electrical Connectors
BNC type connectors are available at the front panel to ease integrating the Pico-Injector with other equipment. Synchronization of injections to other stimulations or recordings is therefore possible.

Selection Guideline
The PLI-100A is available in three packages: Basic, Plus, and Deluxe. The Basic unit is supplied with an input hose, output hose, holding hose, and input hose adapter. The Plus unit also includes a foot switch, pipette holder, and input hose adapter.
The Deluxe unit includes all the accessories included with the Plus and an additional foot switch and pipette holder.

Specifications
Input Gas Pressure 75 PSI Recommended, 105 PSI Maximum
Injection Pressure 0.2 to 60 PSI (413 kPa), regulated
Balance Pressure 0.1 to 9.9 PSI (68.9 kPa) regulated, multiturn control, other ranges available on request
Fill Vacuum Internally produced 0 to -12 PSI (0 to-82 kPa), regulated
Holding Vacuum Internally produced 0 to -0.1 PSI (0 to -0.75 kPa), regulated
Clearing Pressure Input gas pressure, unregulated
Injection Time 0.01 to 99.99 Seconds
Injection Time Accuracy ±0.01% (Crystal Time Base)
Duration Mode Internally timed or externally gated
Time Trigger Front panel, foot switch, or external TTL pulse (BNC)
Pressure Units PSI /kPa switch selectable
Pressure Monitor Output BNC, Rear Panel, 10 mV/PSI or 1 mV/kPa
Pressure Readout Inject, balance, clear, output port
Pressure Display Resolution 3½ Digits, 0.1 PSI or 1 kPa
Injection Count Display 0 to 9999 Injections
Power Input External 9VDC @ 400mA (min) Power Supply universal input 90 to 264 VAC
Weight 5.44 kg (12 lb)
Dimensions (HxWxD) 89 x 432 x 250 mm (H x W x D)
Warranty One year, parts & labor

  PLI rear panel

Rear Panel

Holding Hose with Cell Release Bulb

 Holding Hose with Cell Release Bulb

Output Hose

 Output Hose

Pipette Holder

Pipette Holder (65-0013)

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DOCUMENT DESCRIPTION
PLI-100A_data sheet.pdfPLI-100A Data Sheet
PLI-100A Manual.pdfPicoliter MicroInjector (PLI-100A) Manual