The IPL-1 is engineered for the most demanding applications. The basic system can easily be upgraded to add capability modules for virtually any ex-vivo pulmonary assay. Due to the fragile nature of the mouse lung, its suspension from the tracheal and vascular cannulae is difficult to accomplish without the rapid occurrence of edema. In collaboration with experts in pulmonary physiology, an alternative design was designed which allows for ventilation and perfusion with the lungs remaining in situ at a slight incline. This novel approach simplifies annulation of the pulmonary artery and left atrium of the heart and dramatically reduces edema formation.