Perfusion Fast-Step System (VC-77SP)

Perfusion Fast-Step System (VC-77SP)

This perfusion system is the combination of the VC-6MCS Perfusion Valve Control System and the SF-77B Fast Step Perfusion System.

The system includes:

  • VC-66MCS - Complete 6 channel mini-valve perfusion system
  • SF-77B - Perfusion Fast Step
  • MM-33R - Right handed Micromanipulator
  • MBB - Magnetic Base

Item# Description U.S. List Price Quantity
64-0177 Model VC-77SP Complete VC-6 Fast-Step Perfusion System
64-1519 VC-6 Fast-Step Perfusion System with right-handed micromanipulator, 200-240 VAC, 50/60Hz
64-0177L (VC-77SP/L) VC-6 FastStep perfusion system, with left-handed micromanipulator, 115 VAC
64-0177LE (VC-77SPLE) VC-6 FastStep perfusion system, with left-handed micromanipulator, 220 VAC
64-1590 (VC-77SP8) VC-8 FastStep perfusion system, with right-handed micromanipulator, 115 VAC
64-1590E (VC-77SP8E) VC-8 FastStep perfusion system, with right-handed micromanipulator, 220 VAC
64-0114 Model SF-77B Standard System with MM Series Manifolds, 1 pkg. 3SG700-5 Glass, GH-1 Glass Holder, and 1 pkg. each PE-10 and PE-50 Tubing
69-0225 MB-B Magnetic Base
64-0164 Model TC-3 Tubing Connectors (1/16 in ID), pkg. of 12 straight, 6 Y and 6 Luer Connectors
64-0115 Model SF-77BST Theta Glass System with MM Series Manifolds, 1 pkg. TGC-200-10 Glass, GH-2T Glass Holder, and 1 pkg. each PE-10 and PE-50 Tubing
64-0116 Model SF-77BLT Large Tube System with ML Series Manifolds, 1 pkg. SG1000-5 Glass, GH-10 Glass Holder, and 2 pkg. of PE-50 Tubing
64-1428 Replacement Pipette Tips, pkg of 10
64-0162 Model RS-1 Ring Stand
64-0163 Model SH-6/60 Syringe Holder with six 60 cc Syringes
64-0143 Model SH-8/10 Syringe Holder with eight 10 cc Syringes
64-0144 Model SH-6/140 Syringe Holder with six 140 cc Syringes
64-0165 Model SL-6 Stopcock with Luer Connector, pkg. of 6
64-0385 (SH-8/60) Syringe holder with eight 60 cc syringes
64-1489 (SN-18) Blunt-ended syringe needles, 18G (1.27 mm OD), fits PE-160 tubing, 12 pack
64-1490 (SN-23) Blunt-ended syringe needles, 23G (0.635 mm OD), fits PE-50 tubing, 12 pack
64-0119 Model 3SG700-5 3-Barrel Square Glass Tubes, 0.6 mm x 5 cm (ID x L), pkg. of 10
64-0120 Model 3SG700-10 3-Barrel Square Glass Tubes, 0.6 mm x 10 cm (ID x L), pkg. of 10
64-0121 Model SG-800-5 Single Barrel Square Glass Tubes,0.6 mm x 5 cm (ID x L), pkg. of 25
64-0122 Model SG-1000-5 Single Barrel Square Glass Tubes, 1 mm x 5 cm (ID x L), pkg. of 25
64-0811 Premium Theta Glass, Model TG200-4, Pkg. of 100
64-0139 Model PV-1 Pinch Valve
64-0140 Model TV-2 PTFE Valve
64-0175 Model SV-1 Mini Solenoid Valve
64-0752 PE-50/10 PE-50 Polyethylene Tubing, 10 ft L
64-0753 Model PE-50/100 PE-50 Polyethylene Tubing, 100 ft L
64-0124 Model GH-1 Glass Holder for 3SG700-5, 3SG700-10 and SG800-5 Glass
64-0125 Model GH-2T Glass Holder for Theta Glass
64-0126 Model GH-10 Glass Holder for SG1000-5 Glass
59-8377 Becton Dickinson syringe, 3 cc, 100 per box
59-8378 Becton Dickinson syringe, 5 cc, 100 per box
59-8379 Becton Dickinson syringe, 10 cc, 100 per box, packaged in strips of 5 syringes
59-8380 Becton Dickinson syringe, 20 cc, 40 per box
59-8381 Becton Dickinson syringe, 30 cc, 30 per box
59-8382 Becton Dickinson syringe, 60 cc, 40 per box
72-2399 140 ml Sterile Monoject® Plastic Syringe without Needle, Luer Lock Tip, case of 20
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The VC-77SP is a unique system which enables the user to rapidly select between several perfusion reservoirs, saving time and effort. The system is comprised of the VC-66MCS Valve Control System and the SF-77B Perfusion Fast Step.

The main use of the SF-77B is the rapid change of solutions bathing a single cell or membrane patch attached to a patch electrode. This is typically achieved using a three square barrel piping system, although up to five barrels can be easily accommodated as well as two barrel Theta glass tubing.

Solution changes can be made in as little as 20-50 ms (or as little as ~1 ms using a two-barrel Theta tubing), and once changed the new solution with its specific concentration of pharmacological agent will remain stable until changed again.

An important capability of this device is that a large number of solutions can be used during the course of a single experiment, regardless of the compact size ofVC-77 SP Diagram the device. This creates the ability to use 15-30 different solutions in a single experiment. This feat is accomplished by having as many as six solutions feeding through a unique manifold into each of the three pipes in front of which the cell may be placed. This allows any of the three pipes to have any one of six solutions flowing through it at any time. It is even possible, during the course of an experiment, to change the six solutions flowing into any one of the manifolds, allowing a virtually limitless number of solutions to be used.

The VC-6M Valve Control System lies at the heart of a multi-valve perfusion system designed to automate and control the delivery of small quantities of fluids to Warner Instruments imaging and recording chambers. However, its flexible design allows the system to be used in many applications not using Warner equipment. The miniature solenoid valve assembly is standard with the system. They are lightweight and made from rugged materials for long life.

Valves are dual acting with both normally open and normally closed sides. The normally open side is plugged with removable stoppers and when these are removed solution can flow to waste while the valve is in the off position.

The controller can independently regulate the function of up to six valves. Individual valves can be controlled via a manual switch or an external digital (TTL) signal. Valve transitions (opened or closed) occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions. An event-marker pulse, generated each time a valve is switched on, is provided at the rear of the controller for recording into your acquisition system.

Specifications
Six-Channel Mini-Valves
Valves 12 VDC /0.046 A, solenoid type
Valve manifold PTFE material, mounts on a 3/8" or 1/2" ring stand
Tubing PE-50
Connection Cable 2.4 meter (8 ft.) connecting cable terminated with quick disconnects on valve end and 15 pin male "D" type connector on controller end.
Reservoirs 10 cc capacity syringes
Reservoir Holder Delrin. Holds eight syringes with thumb screws for each reservoir.
Valve Controller Typical response times for valves is 2.5 ms
Switch Selection Manual, Off or External
External Input +5 V TTL-compatible (BNC Connector)
Event Marker Logic level pulse 500 ms nominal (rear panel BNC connector)
Manifold 2/1, 4/1 or 6/1 nominal dead space
Power 110-130 or 200-250 VAC, single-phase, 50/60 Hz, 20 watt
Operating
Temperature
10-40° C (50-104° F)
Dimensions
(H x W x D)
89 x 203 x 305 mm
(3.5 x 8.0 x 12 in.)



 Fast-Step Perfusion System
Number of Steps 1 to 7 (2 to 8 positions)
Step Size Adjustable from 100 µm to 1.5 mm in 100 µm increments, with STEPS/POSITION switch
Step Speed Typically 20 msec for 700 µm step
Step Control
Manual 8 positions with POSITION selector
Analog Signal 5 positions with voltage levels 0-4 V, 1V per step
Digital Signal 8 positions with 3 byte TTL signal
Max. Stepped Range 12.5 mm
Mounting Handle 6.3 mm X 10 cm (D x L)
Stepper Weight 110.5 g (including handle)
Solution
Manifolds
Three manifolds supplied
MM series for SF-77B and SF-77BST
ML series with SF-77 BLT
Manifolds have 2, 4 or 6 inputs
One two input and two six input manifolds are supplied
MM Series MM-2, MM-4 and MM-6 manifolds use PE-50 tubing at input and PE-10 tubing at output
ML Series ML-2, ML-4 and ML-6 manifolds use PE-50 tubing at both input and output
Solution Flow Rates Rates measured with solution reservoir height of approx. 60 cm (24 in)
With MM Series 100 µL/min
With ML Series 1 mL/min
Control Box
Size, H x W x D 6.3 x 14 x 23 cm
Power 100 to 130 or 220 to 250 VAC, 50/60 Hz, 10 VA
Shipping Weight 2.7 kg
Warranty 2 years, parts and labor
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