Open Diamond Bath Imaging Chambers (RC-26, RC-26G)

Open Diamond Bath Imaging Chambers (RC-26, RC-26G)

Large volume open diamond bath recording chamber that can accommodate large specimens such as slice preparations.

  • Designed for patch clamp studies
  • Can be used with tissue slice samples or cell cultured coverslips
  • Applications include patch clamp, intracellular / extracellular recordings, and imaging
  • Diamond shaped bath provides laminar solution flow
  • Large imaging area
  • Can be used with upright or inverted microscopes
  • Tissue slice anchors available

Item# Description U.S. List Price Quantity
64-0234 Large Bath RC-26 Recording Chamber, 170 µl/mm volume
64-0235 Large Bath RC-26G Recording Chamber, 234 µl/mm volume
64-0277 Series 20 Plain Platform, Model P-1
64-0284 Series 20 Heater Platform, Model PH-1
64-1526 PM-1 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1920 (SA-20PS1) Adapter Ring for Pecon Heating Insert P
64-0250 Slice Anchor - Stainless Steel for RC-26 Chamber, 1.0 mm, SHD-26H/10
64-0251 Slice Anchor - Stainless Steel for RC-26 Chamber, 1.5 mm, SHD-26H/15
64-0252 Slice Anchor - Stainless Steel for RC-26 Chamber, 2.0 mm, SHD-26H/2
64-0253 Slice Anchor - Stainless Steel for RC-26G Chamber, 1.0 mm, SHD-26GH/10
64-0254 Slice Anchor - Stainless Steel for RC-26G Chamber, 1.5 mm, SHD-26GH/15
64-0255 Slice Anchor - Stainless Steel for RC-26G Chamber, 2.0 mm, SHD-26GH/2
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0707 CS-22/40 Coverslips, use with RC-22, RC22C, RC-24, RC-24E, RC-24N, RC-26, RC-26G, RC-26GLP, RC-30, and RC-50, pkg of 50
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1400 Right Hand Suction Tube, Series 20 Classic Design for use with RC-22/22C, RC-25/25F, RC-26/26G/26GLP/26Z
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Model RC-26G chamber on a microscope stage with in-line heater and CC-28 cable with thermistor feedback, (a typical set-up).
Model RC-26G chamber shown in a PH-1 heated platform on a SA-NIK stage adapter
RC-26 shown in a PH-1 heated platform

The RC-26 and RC-26G chambers are designed to be used with cells cultured onto glass coverslips, or with tissue and brain slice samples. The broad imaging area of these chambers (12 x 25 mm and 15 x 24 mm for the RC-26 and RC-26G, respectively) provides excellent access for both electrodes and water-immersion objectives. The diamond shaped geometry facilitates laminar solution flow throughout the bath area.

The large bath of these chambers allows a coverslip to be inserted into the top opening and removed at the end of the experiment. The RC-26 and RC-26G chambers can accept a 12 or 15 mm diameter coverslip, respectively. The working volumes, for 1 mm solution height, of the RC-26 and RC-26G model chambers are 170 and 234 ul, respectively.

Large tissue samples can also be placed directly into the chamber. Slice anchor hold downs are available for tissue slice studies. Anchors are available with Lycra thread spacings at 1.0, 1.5, and 2.0 mm.

Both chambers require a platform (P-1, PH-1, or PM-1) and stage adapter to complete the assembly for mounting on a microscope. The chamber floor consists of a 22 x 40 mm coverslip.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.

An application video is available for this product through the JoVE website!
You can view our selection of videos and access full length videos
here.

Specifications

 RC-26RC-26G
Material Polycarbonate Polycarbonate
Bath dimensions
(L x W x H)
25 x 12 x 4 mm 24 x 15 x 4 mm
Outer dimensions
(L x W x H)
40 x 23 x 4 mm 40 x 23 x 4 mm
Volume, by depth 170 μl/mm 234 μl/mm
Bottom coverglass 22 x 40 mm 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)

 RC-26 drawing

  1. Tumor necrosis factor-[alpha]-induced neutral sphingomyelinase-2 modulates synaptic plasticity by controlling the membrane insertion of NMDA receptors
    Wheeler D, Knapp E, Bandaru VV, Wang Y, Knorr D, Poirier C, Mattson MP, Geiger JD, Haughey NJ
    J Neurochem. 2009 Jun;109(5):1237-49. Epub 2009 Mar 14
  2. Metabolic Acidosis Increases Intracellular Calcium in Bone Cells Through Activation of the Proton Receptor OGR1
    Frick KK, Krieger NS, Nehrke K, Bushinsky DA
    J Bone Miner Res. 2009 Feb;24(2):305-13
  3. Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips
    Chaudhuri B, Hörmann F, Lalonde S, Brady SM, Orlando DA, Benfey P, Frommer WB
    Plant J. 2008 Dec;56(6):948-62. Epub 2008 Sep 18
  4. Deranged Calcium Signaling and Neurodegeneration in Spinocerebellar Ataxia Type 3
    Chen X, Tang TS, Tu H, Nelson O, Pook M, Hammer R, Nukina N, Bezprozvanny I
    J Neurosci. 2008 Nov 26;28(48):12713-24
  5. Evaluation of Dimebon in cellular model of Huntington's disease
    Wu J, Li Q, Bezprozvanny I
    Mol Neurodegener. 2008 Oct 21;3:15
  6. Drosophila Painless Is a Ca2+-Requiring Channel Activated by Noxious Heat
    Sokabe T, Tsujiuchi S, Kadowaki T, Tominaga M
    J Neurosci. 2008 Oct 1;28(40):9929-38
  7. Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts
    Yaradanakul A, Wang TM, Lariccia V, Lin MJ, Shen C, Liu X, Hilgemann DW
    J Gen Physiol. 2008 Jul;132(1):29-50. Epub 2008 Jun 18
  8. Mitochondrial ATP-sensitive K+ channels regulate NMDAR activity in the cortex of the anoxic western painted turtle
    Pamenter ME, Shin DS, Cooray M, Buck LT
    J Physiol. 2008 Feb 15;586(4):1043-58. Epub 2007 Dec 13
  9. Pigment Dispersing Factor-Dependent and -Independent Circadian Locomotor Behavioral Rhythms
    Sheeba V, Sharma VK, Gu H, Chou YT, O'Dowd DK, Holmes TC
    J Neurosci. 2008 Jan 2;28(1):217-27
  10. AMPA receptors undergo channel arrest in the anoxic turtle cortex
    Pamenter ME, Shin DS, Buck LT
    Am J Physiol Regul Integr Comp Physiol. 2008 Feb;294(2):R606-13. Epub 2007 Dec 5
  11. Autocrine activation of EGF receptor promotes oscillation of glutamate-induced calcium increase in astrocytes cultured in rat cerebral cortex,
    Mitsuhiro Morita, Nagisa Kozuka, Rurika Itofusa, Masashi Yukawa, Yosihisa Kudo,
    Journal of Neurochemistry Volume 95 Issue 3 Page 871-879, November 2005
  12. Reelin Modulates NMDA Receptor Activity in Cortical Neurons
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    The Journal of Neuroscience, September 7, 2005, 25(36):8209-8216
  13. Src regulates distinct pathways for cell volume control through Vav and phospholipase Cgamma,
    Elisabeth T. Barfod, Ann L. Moore, Richard F. Melnick, and Steven D. Lidofsky
    J. Biol. Chem., Vol. 280, Issue 27, 25548-25557, July 8, 2005
  14. Exocytic Trafficking Is Required for Nicotine-induced Up-regulation of 4 2 Nicotinic Acetylcholine Receptors
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    THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 280, No. 18, Issue of May 6, pp. 18311.18320, 2005
  15. Ileitis modulates potassium and sodium currents in guinea pig dorsal root ganglia sensory neurons
    Stewart T, Beyak MJ, Vanner S
    J Physiol. 2003 Nov 1;552(Pt 3):797-807. Epub 2003 Aug 15
DOCUMENT DESCRIPTION
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-26 26G (080724).pdfRC-26 and RC-26G Chamber Instructions
RC-26 26P 26G (080326.1).pdfRC-26, R-26P and RC-26G Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)