Open Diamond Bath Imaging Chambers (RC-22, RC-22C)
The RC-22 and RC-22C are small volume, open bath recording chambers designed for tissue and brain slice studies.
- Designed for tissue slice samples or cell cultured coverslips
- Applications include patch clamp, intracellular / extracellular recordings, and imaging
- Diamond shaped bath provides laminar solution flow
- Small bath volume facilitates rapid solution exchange
- Tissue slice anchor included
The RC-22 and RC-22C feature an open bath and include a slice anchor. The diamond shape geometry of these chamber baths facilitate laminar solution flow which can provide minimal distortion of the sample during perfusion.
Slices are secured via a supplied stainless steel anchor (or harp). Threads are made of Lycra© and have a spacing of 1.5 mm. The RC-22 is supplied with an SHD-22L/15 anchor, and the RC-22C is supplied with an SHD-22CL/15 anchor. While anchor mass (typically 300 milligrams) may be sufficient to hold a slice in place, Warner anchors rely on a close fit between the anchor body and the chamber sidewall. This feature can reduce stress on the anchored slice.
Solution aspiration is via a stainless steel suction tube which is secured by a clamp. This insures that the adjustable-height suction tube will not move once set by the user. Each chamber has an agar bridge well for reference electrodes. The working volume of the RC-22 and RC-22C is 115 µl and 138 µl, respectively. Chamber sidewalls have a height of 1.2 mm to facilitate electrode access and placement.
A temperature controller TC-344C or TC-324C that connects to the heated platform can be added to set and maintain a certain temperature.
Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.
(L x W x H)
|22 x 8 x 3.8 mm||23 x 10 x 3.8 mm|
|Volume, by depth||115 μl/mm||138 μl/mm|
|Bottom coverglass||22 x 40 mm||22 x 40 mm|
(ID x OD)
|1.14 x 1.57 mm (PE-160)||1.14 x 1.57 mm (PE-160)|
|Aspirator coupling||1.67 mm (OD)||1.67 mm (OD)|