Open Diamond Bath Imaging Chambers for Round Coverslips (RC-25, RC-25F)
Open diamond bath recording chambers designed for round coverslips.
- Designed for physiological measurements of cell cultured coverslips
- Applications include patch clamp, intracellular/extracellular recordings, and imaging
- Uses 12 and 15 mm diameter round coverslips as the chamber floor
- Diamond shaped bath provides laminar solution flow
- Small bath volume facilitates fast solution exchange
The design of the RC-25 and RC-25F chambers incorporates a diamond-shaped bath which has been shown to produce laminar flow. Since the bath volumes of these chambers is small (90 and 133 ul/mm for the RC-25 and RC-25F, respectively), fluid exchange times are measured in seconds even when flow rates are less than 1 ml/min.
The RC-25 works equally well with either 12 or 13 mm diameter coverslips while the RC-25F uses 15 mm coverslips. In general, these coverslips are used to form the bottom of the chamber bath. NOTE: These chambers are not designed for insertion of coverslips from the top.
The RC-25 requires a platform (PM-3, PH-3, or P-3) and stage adapter to complete the assembly for mounting onto a microscope. The chamber is supplied with a package of #1.5 thickness glass coverslips (CS-12R, 12 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.
Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.
The RC-25F requires a platform (PM-4, PH-4, or P-4) and stage adapter to complete the assembly for mounting onto a microscope. The chamber is supplied with a package of #1.5 thickness glass coverslips (CS-15R, 15 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.
(L x W x H)
|25 x 12 x 4.1 mm||24 x 13 x 4.1 mm|
|Volume, by depth||90 μl/mm||133 μl/mm|
|Bottom coverglass||12 mm round||15 mm round|
(ID x OD)
|1.14 x 1.57 mm (PE-160)||1.14 x 1.57 mm (PE-160)|
|Aspirator coupling||1.67 mm (OD)||1.67 mm (OD)|