Oocyte Recording Chamber (RC-1Z)

Oocyte Recording Chamber (RC-1Z)

A simple, low cost oocyte perfusion chamber

  • Specially designed for oocyte studies
  • Unique slotted bath
  • Dimpled bottom to secure oocyte into position

Item# Description U.S. List Price Quantity
64-0318 RC-1Z Oocyte Chamber
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-1586 (BPM-1) Base plate, magnetic steel, 12x24 x 3/8', thick powder coating"
64-0206 MP-2 Perfusion Manifold, 2 Inputs
64-0207 MP-3 Perfusion Manifold, 3 Inputs
64-0208 MP-4 Perfusion Manifold, 4 Inputs
64-0209 MP-5 Perfusion Manifold, 5 Inputs
64-0210 MP-6 Perfusion Manifold, 6 Inputs
64-0211 MP-8 Perfusion Manifold, 8 Inputs
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-1402 Straight Suction Tube, Classic Design for use with RC-1Z chamber
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The RC-1Z chamber features a unique slot-shaped bath for applications requiring rapid perfusion.

The chamber is constructed using a two-piece design. The top piece forms the chamber body and contains a solution aspiration reservoir. An agar bridge well with connecting channel to the input side of the bath is also provided. An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.

The bottom piece forms the floor of the chamber and houses the oocyte work area. To aid in securing the oocyte into position during impalement, the chamber bottom has three dimples with diameters of 0.8, 0.9, and 1.0 mm. Three grooves milled into the chamber bottom (parallel to the direction of solution flow) aid in bathing the bottom side of the oocyte during perfusion.

Solutions are removed from the suction well via an adjustable suction tube and bath solution height is adjusted by raising or lowering the suction tube. The perfusion input accepts standard 1/16 OD (PE-160) polyethylene tubing

Specifications
Material polycarbonate
Footprint
(L x W)
7.7 x 5.1 cm
Bath Dimensions
(L x W x H)
25 x 3.5 x 2.5 mm
Volume, by depth 85 μl/mm
Chamber Floor Insert 25 x 75 x 3 mm, polycarbonate
Input Tubing (ID x OD) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)
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  8. Loop Gating of Connexin Hemichannels Involves Movement of Pore-lining Residues in the First Extracellular Loop Domain Verselis. VK, Trelles MP, Rubinos C, Bargiello TA, Srinivas M J Biol Chem. 2009 Feb 13;284(7):4484-93. Epub 2008 Dec 11.
  9. Divalent Cations Regulate Connexin Hemichannels by Modulating Intrinsic Voltage-dependent Gating. Verselis VK, Srinivas M J Gen Physiol. 2008 Sep;132(3):315-27. Epub 2008 Aug 11.
  10. Morantel Allosterically Enhances Channel Gating of Neuronal Nicotinic Acetylcholine alpha 3 beta 2 Receptors. Wu TY, Smith CM, Sine SM, Levandoski MM Mol Pharmacol. 2008 Aug;74(2):466-75. Epub 2008 May 5.
  11. 1,4-Diazabicyclo[2.2.2]octane Derivatives: A Novel Class of Voltage-Gated Potassium Channel Blockers. Gordon E, Cohen JL, Engel R, Abbott GW Mol Pharmacol. 2006 Mar;69(3):718-26. Epub 2005 Nov 29.
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  13. Transport of the Natural Sweetener Stevioside and Its Aglycone Steviol by Human Organic Anion Transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). Srimaroeng C, Chatsudthipong V, Aslamkhan AG, Pritchard JB J Pharmacol Exp Ther. 2005 May;313(2):621-8. Epub 2005 Jan 11.
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DOCUMENT DESCRIPTION
RC-1Z Manual.pdfRC-1Z Manual