Oocyte Clamp (OC-725C)

Oocyte Clamp (OC-725C)

The OC-725C Oocyte Clamp is designed for two-electrode, whole-cell voltage clamping of Xenopus oocytes.

  • High compliance voltage (±180 V)
  • Unique bath clamp circuitry
  • Ideal for clamping large cells and cell structures e.g. squid axons
  • Extended current measuring range
  • Decreased noise level and 4-pole Bessel filter
  • An internal switch permits measurements of the current in series with the current electrode instead of in the bath
  • Optional differential voltage headstages
  • Voltage and current electrode holders must be purchased separately

Item# Description U.S. List Price Quantity
64-0028 OC-725C Oocyte Clamp Supplied with 7250V voltage headstage, 7251I bath clamp headstage, 7259C current cable, model membrane and rack mount hardware. Specify line operating voltage if other than 100-130 VAC. One straight holder and one 45° holder nee
64-1007 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.0 mm Glass ID
64-1008 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.2 mm Glass ID
64-1009 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.5 mm Glass ID
64-1010 E Series Straight Body Electrode Holder; Wire w/ 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 2.0 mm Glass ID
64-1051 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.0 mm Glass OD
64-1052 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.2 mm Glass OD
64-1053 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 1.5 mm Glass OD
64-1054 E Series with Handle; Wire with 2 mm Jack and Vent (for use with Warner Oocyte Clamp OC-725), 2.0 mm Glass OD
64-0185 Model VC-8P Valve Control System/8 pinch valves
64-0135 Model VC-66CS Complete Pinch Valve System with 6 Valves
64-1940 (DWV) Dedicated Workstation Vacuum
69-0130 Nanoject II Nanoliter Injector, 115 V, 60 Hz, US Plug
64-1586 (BPM-1) Base plate, magnetic steel, 12x24 x 3/8', thick powder coating"
72-0311 NCL 150, 120V/60Hz
72-0267 Bifurcated Gooseneck Light Guide, Black, 4.7 mm fiber diameter (each arm)
64-1803 Binocular Zoom Stereo Microscope, 0.8-5.0x zoom, with Boom Stand
64-1328 WA30-5 1 mm Pin with 30 cm L, 26 ga. Insulated Wire, pkg. of 3
64-0029 725MC Model Cell for Oocyte Clamp
64-1327 WA10-5 1 mm Pin with 10 cm L, .25 mm D Bare Silver Wire, pkg. of 2
64-1289 Pipette Seal for 1.0 m Diameter Glass
64-1290 Pipette Seal for 1.2 m Diameter Glass
64-1291 Pipette Seal for 1.5 m Diameter Glass
64-1292 Pipette Seal for 2.0 m Diameter Glass
64-1298 Wire Seal for E, ME, MP, and Theta Holders
463-1180 Probe handle assembly for OC-725 Series voltage electrode
460-8090 (2U) Rack handle
64-0455 (PV-830) Power Line Conditioner
1000 VA, 120 VAC 60HZ
64-0456 (PV-830CE) Power Line Conditioner 1000 VA, 220 VAC 50/60HZ
64-0031 7250V Replacement Voltage Headstage for Oocyte Clamp
64-0032 7251I Replacement Bath Clamp Headstage for Oocyte Clamp
64-0033 7259C Replacement Current Electrode Cable for Oocyte Clamp
64-0030 7255DI Optional Differential Headstage for Oocyte Clamp
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Fast Stable Voltage Clamping

The OC-725C combines high AC and DC gains and a voltage compliance of ±180 volts to insure fast, non-saturating clamp performance under nearly any condition. The AC clamp gain is variable up to 2000. An additional DC gain of 1 x 106 may be employed for high conductance cells (leaky Oocytes).

Two clamp speeds are available: The Slow mode is used for screening oocytes or for applications not requiring fast response times. The Fast mode is used for accurate voltage clamp of fast whole cell currents. Clamp response time in the Fast mode is 350 µsec (10-90% rise time) when applying a 100 mV step to a model cell.

Improved Bath Clamp Headstage

The current measuring range of the OC-725C bath clamp headstage has been extended at both ends by the addition of a 3 position range multiplier. Smaller currents are amplified to usable levels and larger currents up to 1 mA can be recorded without output saturation. The unique design of the bath clamp eliminates the need for series resistance compensation. It provides an accurate measurement of bath current by creating a virtual ground in the bath while simultaneously clamping the bath potential at zero.

Voltage Headstage Probe

The voltage measuring headstage is a single-ended, high-impedance probe. Its small size, convenient mounting rod and 2 meter cable make for easy attachment to a micropositioner. The headstage input is a 2 mm diameter pin. An electrode holder with a 2 mm jack (supplied) mounts directly on the headstage.

Voltage and Current Meters

Independent meters provide simultaneous displays of membrane voltage Vm and membrane current Im. To assure proper impalement of the current electrode, the current meter displays membrane potential Ve from the current electrode before the clamp circuit is turned on.

Clamp Commands

The internal Hold control is a digital push button control with two ranges; ±1 to 99 mV (x1) and ±2 to 198 mV (x2). Hold can be incremented in steps of 1, 2, 10 and 20 mV for I-V studies. External command signals applied to Command IN ÷10 are attenuated to reduce noise from the command source. Hold and external commands are summed.

Additional Features

  • Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage
  • Overload alarm (audible and visual) indicate when the compliance voltage is exceeded safeguarding the Oocyte and indicating that current records are subject to saturation
  • DC Offsets for both voltage and current electrodes
  • Electrode Test for both electrodes
  • Capacity Compensation for the Vm voltage input

Electrode Holders

Two vented electrode holders with silver wires are typically required with the clamp; a straight type for use with the voltage headstage and a 45° type with mounting handle for use with current electrodes. Vents have been added to the electrodes to prevent pressure build-up inside the electrode which can damage oocytes.

Unvented holders are available and have a 'N' at the end of the model number. Vented holders have a 'V' on the end of the model number. A two meter length cable assembly is provided to connect the current electrode holder to the clamp.

Select the holder based on your glass outer diameter. Holders are available separately.

Dual Oocyte Studies

Some studies, such as voltage control across gap junctions, require the clamping of two oocytes in a common bath using two voltage clamps. The combined currents from the two Oocytes cause problems because the bath clamp headstage cannot separate the individual currents and therefore cannot provide effective clamping.

OC-725C has two features to address these problems:

  • An internal switch permits measurements of the current in series with the current electrode instead of in the bath
  • Optional differential voltage headstages (Model 7255 DI): The differential measurement subtracts the voltage drop across the series resistance in the bath, which is normally eliminated by the bath clamp

Supplied Hardware

The OC-725 is supplied with:

  • 7250V voltage headstage
  • 7251I bath clamp headstage
  • 7259C current cable
  • Model cell, rack mount hardware
  • Specify line operating voltage if other than 100-130 VAC

Purchased separately

  • One straight and one 45º electrode holder is also needed
Specifications
Test Condition #1 Model cell used to obtain specifications.
Model membrane: 1 MΩ in parallel with 220 nF.
Current and voltage electrodes both 1 MΩ impedence.
Test Condition #2 Noise measurements made with an 8-pole Bessel filter.
Voltage Recording Channel (Vm) V Probe Input Impedance 0.5 x 1012Ω, 1 pF
Output Resistance 100Ω
DC Offset ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn).
Noise (0-10 kHz) 3 µV RMS with input grounded
20 µV RMS with model cell
Electrode Test 10 mV/MΩ read on meter
100 mV/MΩ at Vm x10 output
Meter Range ± 199.9 mV full scale
Capacity Compensation 0 - 90 pF
Current Sensing Channel (Bath Clamp) (Im) Noise (0-1 kHz) 4.5 nA RMS with bath clamp
10 nA RMS in output leg
I Monitor Output 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10.
Gain Telegraph Output 0.2 to 2.6 V in 7 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software*
Meter Range, full scale Clamp Current: ± 199.9 µA
Electrode Voltage (Ve) ± 199.9 mV; current meter reads Ve when clamp mode switch is off.
Current Electrode Channel Compliance Voltage ± 180 V
Clamp Speed 350 µsec; (10-90%) with 100 mV square wave command applied to model cell
Gain Variable AC/DC: 0 to 2000
Fixed DC Gain Switch selected: 1 x 106
Ve DC Offset ± 200 mV at input (20 mV/turn)
Electrode Test 10 mV/MΩ read on current meter
100 mV/MΩ at Ve x10 output rear panel
Commands Hold Manually set with digital potentiometer, 2 digit resolution and 2 ranges:
x1 range: ± 1 to 99 mV in 1 mV steps
x2 range: ± 2 to 198 mV in 2 mV steps
External Signals applied to COMMAND IN÷10 are attenuated by a factor of 10,
1 V applied = 100 mV command
Physical Dimensions Case 8.9 cm H x 43.2 cm W x 30.5 cm D
Voltage Headstage 12.5 mm D x 5 cm L with 1.8 m cable
Mounting Handle 4.8 mm D x 6.3 cm L
Bath Headstage 2.3 cm H x 3.5 cm W x 4.2 cm L with 1.8 m cable
Shipping Weight 6.8 kg
Warranty Three years, parts & labor
Power Requirements 100-130 V or 220-240 VAC, 50/60 Hz, 20 VA
  1. On-Demand Webinar: Data Acquisition and Recording with TEV-700 Oocyte Workstation
    Warner Instruments
    Vimeo 2013
References
  1. A presynaptic endosomal trafficking pathway controls synaptic growth signaling.
    Rodal AA, Blunk AD, Akbergenova Y, Jorquera RA, Buhl LK, Littleton JT.
    J Cell Biol. 2011 Apr 4;193(1):201-17.
  2. DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization.
    Schulte J, Sepp KJ, Jorquera RA, Wu C, Song Y, Hong P, Littleton JT.
    J Neurosci. 2010 Apr 14;30(15):5189-203.
  3. Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains.
    Barber CF, Jorquera RA, Melom JE, Littleton JT.
    J Cell Biol. 2009 Oct 19;187(2):295-310. Epub 2009 Oct 12.
  4. Oviduct contraction in Drosophila is modulated by a neural network that is both, octopaminergic and glutamatergic.
    Rodríguez-Valentín R, López-González I, Jorquera R, Labarca P, Zurita M, Reynaud E.
    J Cell Physiol. 2006 Oct;209(1):183-98.
  5. Drosophila melanogaster Scramblases modulate synaptic transmission.
    Acharya U, Edwards MB, Jorquera RA, Silva H, Nagashima K, Labarca P, Acharya JK.
    J Cell Biol. 2006 Apr 10;173(1):69-82.
  6. Deranged Calcium Signaling and Neurodegeneration in Spinocerebellar Ataxia Type 2
    Jing Liu, Tie-Shan Tang, Huiping Tu, Omar Nelson, Emily Herndon, Duong P. Huynh, Stefan M. Pulst, and Ilya Bezprozvanny
    The Journal of Neuroscience, July 22, 2009, 29(29):9148-9162
  7. Alphaxalone, a neurosteroid anaesthetic, increases the activity of the glutamate transporter type 3 expressed in Xenopus oocytes
    Junghee Ryua, Il-Young Cheongb, Sang-Hwan Do, and Zhiyi Zuo
    European Journal of Pharmacology Volume 602, Issue 1, 5 January 2009, Pages 23-27
  8. Ion binding in the open HCN pacemaker channel pore: fast mechanisms to shape "slow" channels.
    Lyashchenko AK, Tibbs GR.
    J Gen Physiol. 2008 Mar;131(3):227-43.
  9. Functional Characterization of a Novel CFTR Mutation P67S Identified in a Patient with Atypical Cystic Fibrosis,
    Cornelia Kraus, André Reis, Lutz Naehrlich, Jörg Dötsch, Christoph Korbmacher and Robert Rauh
    Cell Physiol Biochem 2007;19:239-248
  10. Functional analysis of a novel potassium channel (KCNA1) mutation in hereditary myokymia
    Haijun Chen, Christian von Hehn, Leonard K. Kaczmarek, Laura R. Ment Barbara R. Pober, Fuki M. Hisama,
    Neurogenetics (2007) 8:131 135
  11. The delta subunit of gamma-aminobutyric acid type A receptors does not confer sensitivity to low concentrations of ethanol
    Cecilia M. Borghese, Signe ´ Sto´ rustovu, Bjarke Ebert, Murray B. Herd, Delia Belelli, Jeremy J. Lambert, George Marshall, Keith A. Wafford, and R. Adron Harris,
    THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 316:13601368, 2006
  12. Electrogenic Na/HCO3 Cotransporter (NBCe1) in Xenopus Oocytes: Functional Comparison and Roles of the AminoVariants Expressed and Carboxy Termini
    Suzanne D. McAlear, Xiaofen Liu, Jennifer B. Williams, Carmel M. McNicholas-Bevensee, and Mark O. Bevensee,
    The Rockefeller University Press, JGP, Volume 127, Number 6, 639-658
  13. Effect of Human Carbonic Anhydrase II on the Activity of the Human Electrogenic Na/HCO3 Cotransporter NBCe1-A in Xenopus Oocytes
    Jing Lu, Christopher M. Daly, Mark D. Parker, Harindarpal S. Gill, Peter M. Piermarini, Marc F. Pelletier, and Walter F. Boron,
    THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 28, pp. 1924119250, July 14, 2006
  14. Interaction of KCNE subunits with the KCNQ1K+ channel pore,
    Gianina Panaghie Kwok-Keung Tai and Geoffrey W. Abbott,
    J Physiol 570.3 (2006) pp 455467
  15. Carboxyl-Terminal Splicing Enhances Physical Interactions between the Cytoplasmic Tails of Purinergic P2X Receptors
    Taka-aki Koshimizu, Karla Kretschmannova, Mu-Lan He, Susumu Ueno, Akito Tanoue, Nobuyuki Yanagihara, Stanko S. Stojilkovic, and Gozoh Tsujimoto,
    Mol Pharmacol 69:15881598, 2006
  16. Podocin and MEC-2 bind cholesterol to regulate the activity of associated ion channels
    Tobias B. Huber, Bernhard Schermer, Roman Ulrich Müller, Martin Höhne, Malte Bartram, ect.
    PNAS | November 14, 2006 | vol. 103 | no. 46 | 17079-17086
  17. A Molecular Determinant of Nickel Inhibition in Cav3.2 T-type Calcium Channels
    Ho-Won Kang1, Jin-Yong Park, Seong-Woo Jeong, Jin-Ah Kim, Hyung-Jo Moon, Edward Perez-Reyes, and Jung-Ha Lee
    Biol. Chem., Vol. 281, Issue 8, 4823-4830, February 24, 2006
  18. Endogenous KCNE Subunits Govern Kv2.1 K1 Channel Activation Kinetics in Xenopus Oocyte Studies
    Earl Gordon, Torsten K. Roepke, and Geoffrey W. Abbott Earl Gordon, Torsten K. Roepke, and Geoffrey W. Abbott,
    Biophysical Journal Volume 90 February 2006 12231231
  19. Functional Roles of Charged Amino Acid Residues on the Wall of the Cytoplasmic Pore of Kir2.1
    Yuichiro Fujiwara1 and Yoshihiro Kubo
    The Journal of General Physiology, 2006 Volume 127, Number 4, 401-419
  20. Sugar Solution Analgesia: The Effects of Glucose on Expressed Mu Opioid Receptors,
    George R. Kracke, PhD, Katherine A. Uthoff, BS, and Joseph D. Tobias, MD
    Anesth Analg 2005;101:64-68
  21. Downregulation of Transient Receptor Potential Melastatin 8 by Protein Kinase C-Mediated Dephosphorylation
    Louis S. Premkumar, Manish Raisinghani, Sandeep C. Pingle, Cheng Long, and Fátima Pimentel,
    The Journal of Neuroscience, December 7, 2005, 25(49):11322-11329
  22. Dynamic conformational changes of extracellular S5.P linkers in the hERG channel
    Min Jiang, Mei Zhang, Innokenty V. Maslennikov, Jie Liu, Dong-Mei Wu, Yuliya V. Korolkova, Alexander S. Arseniev, Eugene V. Grishin, Gea-Ny Tseng
    The Journal of Physiology Volume 569 Issue 1 Page 75-89, November 2005
  23. Sensitization and translocation of TRPV1 by insulin and IGF-I,
    Jeremy J Van Buren1, Satyanarayan Bhat1, Rebecca Rotello1, Mary E Pauza,
    Molecular Pain 2005, 1:17
  24. Tweaking Agonist Efficacy at N-Methyl-D-aspartate Receptors by Site-Directed Mutagenesis
    Kasper B. Hansen, Rasmus P. Clausen, Esben J. Bjerrum, Christian Bechmann, Jeremy R. Greenwood, Caspar Christensen, Jesper L. Kristensen, Jan Egebjerg, and Hans Bräuner-Osborne
    Mol Pharmacol 68:1510-1523, 2005
  25. Protein kinase C shifts the voltage dependence of KCNQ/M channels expressed in Xenopus oocytes
    The Journal of Physiology Volume 567 Issue 3 Page 771 Issue 3 - 786 - September 2005
  26. Voltage-sensor activation with a tarantula toxin as cargo
    L. Revell Phillips1, Mirela Milescu1, Yingying Li-Smerin, Joseph A. Mindell, Jae Il Kim, Kenton J. Swartz
    Nature Vol 436|11 August 2005
  27. Phosphorylation-regulated endoplasmic reticulum retention signal in the renal outer-medullary K+ channel (ROMK),
    Anthony D. O'Connell, Qiang Leng, Ke Dong, Gordon G. MacGregor, Gerhard Giebisch, and Steven C. Hebert
    PNAS | July 12, 2005 | vol. 102 | no. 28 | 9954-9959
  28. A New Kindred With Pseudohypoaldosteronism Type II and a Novel Mutation (564D>H) in the Acidic Motif of the WNK4 Gene
    Amir P. Golbang; Meena Murthy; Abbas Hamad; Che-Hsiung Liu; Georgina Cope; William Vant Hoff; Alan Cuthbert; Kevin M. O'Shaughnessy
    Hypertension. 2005;46:295
  29. Substrate interactions in the human type IIa sodium-phosphate cotransporter (NaPi-IIa)
    Leila V. Virkki, Ian C. Forster, Jürg Biber, and Heini Murer
    Am J Physiol Renal Physiol 288: F969-F981, 2005
  30. Zinc inhibition of gamma-aminobutyric acid transporter 4 (GAT4) reveals a link between excitatory and inhibitory neurotransmission
    Einav Cohen-Kfir, William Lee, Sepehr Eskandari, and Nathan Nelson,
    PNAS | April 26, 2005 | vol. 102 | no. 17 | 6154-6159
  31. Deorphanization of GPRC6A: a promiscuous L-alpha-amino acid receptor with preference for basic amino acids
    Petrine Wellendorph, Kasper B. Hansen, Anders Balsgaard, Jeremy R. Greenwood, Jan Egebjerg, and Hans Bräuner-Osborne
    Mol Pharmacol 67:589-597, 2005
  32. Nedd42 isoforms differentially associate with ENaC and regulate its activity,
    Omar A. Itani, John B. Stokes, and Christie P. Thomas
    Am J Physiol Renal Physiol 289: F334-F346, 2005
  33. Multiprotein assembly of Kv4.2, KChIP3 and DPP10 produces ternary channel complexes with ISA-like properties
    Henry H. Jerng, Kumud Kunjilwar, Paul J. Pfaffinger
    The Journal of Physiology Volume 568 Issue 3 Page 767-788, November 2005
  34. Characteristics of Ginsenoside Rg3-Mediated Brain Na+ Current Inhibition
    Jun-Ho Lee, Sang Min Jeong, Jong-Hoon Kim, Byung-Hwan Lee, In-Soo Yoon, Joon-Hee Lee, Sun-Hye Choi, Dong-Hyun Kim, Hyewhon Rhim, Sung Soo Kim, Jai-Il Kim, Choon-Gon Jang, Jin-Ho Song, and Seung-Yeol Nah
    Mol Pharmacol 68:1114-1126, 2005
  35. Epithelial Sodium Channel Inhibition by AMP-activated Protein Kinase in Oocytes and Polarized Renal Epithelial Cells
    Marcelo D. Carattino, Robert S. Edinger, Heather J. Grieser, Rosalee Wise, Dietbert Neumann, Uwe Schlattner, John P. Johnson, Thomas R. Kleyman, and Kenneth R. Hallows
    J. Biol. Chem., Vol. 280, Issue 18, 17608-17616, May 6, 2005
  36. Coupling between Voltage Sensors and Activation Gate in Voltage-gated K+ Channels
    Zhe Lu, Angela M. Klem and Yajamana Ramu
    Journal of General Physiology, Volume 120, Number 5, November 2002 663-676
  37. Analysis of Mecamylamine Stereoisomers on Human Nicotinic Receptor Subtypes
    Roger L. Papke, Paul R. Sanberg and R. Douglas Shytle
    Pharmacology, Vol. 297, Issue 2, 646-656, May 2001
  38. Ca2+-sensitive Inactivation and Facilitation of L-type Ca2+ Channels Both Depend on Specific Amino Acid Residues in a Consensus Calmodulin-binding Motif in thealpha 1C subunit
    Roger D. ZühlkeDagger, Geoffrey S. Pitt, Richard W. Tsien, and Harald Reuter
    J. Biol. Chem., Vol. 275, Issue 28, 21121-21129, July 14, 2000
  39. Helical Structural Elements within the Voltage-sensing Domains of a K+ Channel
    Yingying Li-Smerina, David H. Hackosa, and Kenton J. Swartz
    The Journal of General Phyiology, Volume 115, Number 1, January 1, 2000 33-50
  40. Capsaicin Binds to the Intracellular Domain of the Capsaicin-Activated Ion Channel
    Jooyoung Jung, Sun Wook Hwang, Jiyeon Kwak, Soon-Youl Lee, Chang-Joong Kang, Won Bae Kim, Donghee Kim, and Uhtaek Oh
    The Journal of Neuroscience, January 15, 1999, 19(2):529-538
  41. alpha 5 Subunit Alters Desensitization, Pharmacology, Ca++ Permeability and Ca++ Modulation of Human Neuronal alpha 3 Nicotinic Receptors1
    Volodymyr Gerzanich, Fan Wang, Alexander Kuryatov and Jon Lindstrom
    Pharmacology and Experimental Therapeutics, Vol. 286, Issue 1, 311-320, July 1998
  42. High-Affinity Zinc Inhibition of NMDA NR1-NR2A Receptors
    Pierre Paoletti, Philippe Ascher, and Jacques Neyton
    Volume 17, Number 15, Issue of August 1, 1997 pp. 5711-5725
  43. Localization and Functional Expression of Splice Variants of the P2X2 Receptor
    Joseph Simon, Emma J. Kidd, Fiona M. Smith, Iain P. Chessell, Ruth Murrell-Lagnado, Patrick P. A. Humphrey, and Eric A. Barnard
    MOLECULAR PHARMACOLOGY 52:237-248 (1997)
  44. Chronic Nicotine Exposure Differentially Affects the Function of Human alpha 3, alpha 4, and alpha 7 Neuronal Nicotinic Receptor Subtypes1
    Felix Olale, Volodymyr Gerzanich, Alexander Kuryatov, Fan Wang and Jon Lindstrom
    Pharmacology and Experimental Therapeutics, Vol. 283, Issue 2, 675-683, 1997
  45. Two Isoforms of the Mouse Ether-a-go-go–Related Gene Coassemble to Form Channels With Properties Similar to the Rapidly Activating Component of the Cardiac Delayed Rectifier K+ Current
    Barry London, Matthew C. Trudeau, Kimberly P. Newton, Anita K. Beyer, Neal G. Copeland, Debra J. Gilbert, Nancy A. J enkins, Carol A. Satler, Gail A. Robertson
    Circulation Research. 1997;81:870-878.
  46. Comparative pharmacology of epibatidine: a potent agonist for neuronal nicotinic acetylcholine receptors
    V Gerzanich, X Peng, F Wang, G Wells, R Anand, S Fletcher and J Lindstrom
    Pharmacology and Experimental Therapeutics, Volume 48, October 1995, Issue 4, pp. 774-782
  47. A Novel Subunit Increases Rate of Inactivation of Specific Voltage-gated Potassium Channel Subunits
    Michael J. Morales, Robert C. Castellino, Anne L. Crews, Randall L. Rasmusson, Harold C. Strauss
    The Journal of Biological Chemistry Volume 270, Number 11, Issue of March 17, 1995 pp. 6272-6277
  48. Cloning and expression of a third calcium channel beta subunit
    A Castellano, X Wei, L Birnbaumer and E Perez-Reyes
    J. Biol. Chem., Vol. 268, Issue 5, 3450-3455, 02, 1993
  49. Cloning and expression of a cardiac/brain beta subunit of the L-type calcium channel
    E Perez-Reyes, A Castellano, HS Kim, P Bertrand, E Baggstrom, AE Lacerda, XY Wei and L Birnbaumer
    J. Biol. Chem., Vol. 267, Issue 3, 1792-1797, 01, 1992
  50. Heterologous regulation of the cardiac Ca2+ channel alpha 1 subunit by skeletal muscle beta and gamma subunits. Implications for the structure of cardiac L-type Ca2+ channels
    XY Wei, E Perez-Reyes, AE Lacerda, G Schuster, AM Brown and L Birnbaumer
    J. Biol. Chem., Vol. 266, Issue 32, 21943-21947, 11, 1991
DOCUMENT DESCRIPTION
OC-725C (070514).pdfOC-725C Manual
Connecting the OC-725 to pclamp (2005.06.24).pdfConnecting the OC-725 to pClamp