Narrow Bath Chamber with Field Stimulation (RC-27NE2)

Narrow Bath Chamber with Field Stimulation (RC-27NE2)

This chamber has a narrower bath and smaller volume than the RC-27. It also has embedded electrodes to support studies using field stimulation.

  • Designed for field stimulation studies
  • Rectangular, open-bath chamber
  • Narrow bath
  • Accommodates tissue or brain slice specimens
  • Applications include patch clamp, and physiological measurements on cultured cells

Item# Description U.S. List Price Quantity
64-0240R2 (RC-27NE2) Narrow Bath Recording Chamber w/ Field Stimulation
64-0282 Series 20 Plain Platform, Model P-6
64-0289 Series 20 Heater Platform, Model PH-6
64-1527 PM-6 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1425 CC-102 Cable, male banana to 1 mm jacks (for use with Warner field stimulation chambers)
64-1942 (SE-27NE-REP) Replacement Stimulation Electrodes for RC-27NE2
64-0709 CS-24/50 Coverslips for use with RC-27, RC-27L, RC-27N, RC-27NE, RC-28, and RC-29, 0.15 mm (0.006 in)24 x 50 mm, pkg of 65
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-1401 Left Hand Suction Tube, Series 20 Classic Design for use with RC-27, RC-27L, RC-27NE
64-1429 Suction Tube Upgrade Kit for Series 20 Chambers
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The RC-27NE2 recording chamber is a modified version of the RC-27 with a narrower bath and smaller volume designed for applications requiring electric field stimulation. Laminar solution flow is more stable in this chamber than in the wider RC-27 chamber.

The working specimen area is 28 x 8 mm and there are inclined surfaces on both sides (from 2.0 mm to 3.1 mm height). The low profile design and inclined surfaces permits good electrode access to the specimen area.

The chamber is supplied with a pair of platinum wire electrodes installed along the parallel long sides of the bath. Electrode leads are approximately 16 cm long and terminate with 1 mm diameter pins.

The optional SIU-102 is a bipolar stimulator designed for use with field stimulation chambers. This instrument features constant current and constant voltage, as well as bipolar, pulse and DC modes. Optical coupling is used to isolate the stimulator from pulse source. Currents up to 100 mA and voltages up to 100 V are supported.

The RC-27NE2 chamber requires a platform (P-6, PH-6, or PM-6) and stage adapter to complete the assembly for mounting onto a microscope. The chamber body is manufactured from polycarbonate and the chamber floor is formed from a 24 x 50 mm coverslip.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, pkg. of 50) and 10 ft of PE-160 polyethylene tubing.

Specifications
Material Polycarbonate
Bath dimensions
(L x W x H)
37 x 8 x 2 mm
Outer dimensions
(L x W x H)
50 x 25 x 8-4 mm
Volume, by depth 305 μl/mm
Electrodes Platinum wire
16 cm cables with 1 mm pins
Bottom coverglass 24 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

RC-27NE2 diagram

  1. Multiple pathological events in exercised dystrophic mdx mice are targeted by pentoxifylline: outcome of a large array of in vivo and ex vivo tests
    Burdi R, Rolland JF, Fraysse B, Litvinova K, Cozzoli A, Giannuzzi V, Liantonio A, Camerino GM, Sblendorio V, Capogrosso RF, Palmieri B, Andreetta F, Confalonieri P, De Benedictis L, Montagnani M, De Luca A
    J Appl Physiol. 2009 Apr;106(4):1311-24
  2. Gentamicin treatment in exercised mdx mice: Identification of dystrophin-sensitive pathways and evaluation of efficacy in work-loaded dystrophic muscle
    De Luca A, Nico B, Rolland JF, Cozzoli A, Burdi R, Mangieri D, Giannuzzi V, Liantonio A, Cippone V, De Bellis M, Nicchia GP, Camerino GM, Frigeri A, Svelto M, Camerino DC.
    Neurobiol Dis. 2008 Nov;32(2):243-53
  3. Fluvastatin and Atorvastatin Affect Calcium Homeostasis of Rat Skeletal Muscle Fibers in Vivo and in Vitro by Impairing the Sarcoplasmic Reticulum/Mitochondria Ca2+-Release System
    Liantonio A, Giannuzzi V, Cippone V, Camerino GM, Pierno S, Camerino DC.
    J Pharmacol Exp Ther. 2007 May;321(2):626-34
  4. Niflumic acid inhibits chloride conductance of rat skeletal muscle by directly inhibiting the CLC-1 channel and by increasing intracellular calcium
    Liantonio A, Giannuzzi V, Picollo A, Babini E, Pusch M, Conte Camerino D
    Br J Pharmacol. 2007 Jan;150(2):235-47
  5. Role of tumour necrosis factor [alpha], but not of cyclo-oxygenase-2-derived eicosanoids, on functional and morphological indices of dystrophic progression in mdx mice: a pharmacological approach
    Pierno S, Nico B, Burdi R, Liantonio A, Didonna MP, Cippone V, Fraysse B, Rolland JF, Mangieri D, Andreetta F, Ferro P, Camerino C, Zallone A, Confalonieri P, De Luca A
    Neuropathol Appl Neurobiol. 2007 Jun;33(3):344-59
DOCUMENT DESCRIPTION
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-27NE2 (120607).pdfRC-27NE2 Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)