Multichannel Epithelial Voltage Clamps (VCC-MC)

Multichannel Epithelial Voltage Clamps (VCC-MC)

The VCC-MC family of epithelial voltage/current clamps are ideal instruments for use with multichannel chamber systems. Configurations range from 2 to 8 amplifiers. These systems integrate fully with the Acquire and Analyze software package.

  • Not available separately; amplifiers only available as part of full systems
  • High common mode rejection
  • High differential input immediacy
  • Four-stage adjustable frequency response
  • Includes internal pulse generator

How to order (Please see Item Listing):
Select System
Each system includes heater block/stand; chambers; needle valves; electrodes; amplifiers; and software.
Select Inserts
P2300 series inserts fit the Standard and Rat systems; P2400 series inserts fit the Low Volume systems.
Select FN-15 Filling Needles
Electrode tips; and thermocirculator (if needed)
Select P2300 Chamber Caps
If using radioligands or toxic chemicals

Please see the Full Description for complete information about Multichannel Epithelia Voltage Clamps and related products.

 

Please see Full Description for complete details and ordering tips.

Item# Description U.S. List Price Quantity
69-3217 EM2-C: 2-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3220 EM4-C: 4-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3223 EM6-C:: 6-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3226 EM8-C: 8-channel EasyMount system for tissue & cultured cells, standard volume (2.0 - 8.0 ml)
69-3253 EM2-C-HV 2-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3256 EM4-C-HV 4-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3259 EM6-C-HV 6-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3219 EM2-R: 2-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3262 EM8-C-HV 8-channel EasyMount system for tissue & cultured cells, high voltage, standard volume (2.0 - 8.0 ml)
69-3222 EM4-R: 4-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3225 EM6-R: 6-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3228 EM8-R: 8-channel EasyMount system for rat intestinal tissue (2.0 - 8.0 ml)
69-3255 EM2-R:-HV 2-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3258 EM4-R:-HV 4-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3261 EM6-R:-HV 6-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3264 EM8-R:-HV 8-channel EasyMount system for rat intestinal tissue, high voltage (2.0 - 8.0 ml)
69-3218 EM2-LV: 2-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3221 EM4-LV: 4-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3224 EM6-LV: 6-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3227 EM8-LV: 8-channel EasyMount system for tissue & cultured cells, low volume (0.5 - 5.0 ml)
69-3254 EM2-LV-HV: 2-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3257 EM4-LV-HV: 4-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3260 EM6-LV-HV: 6-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3263 EM8-LV-HV: 8-channel EasyMount system for tissue & cultured cells, low volume, high voltage (0.5 - 5.0 ml)
69-3229 EM2-C-CE: 2-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3232 EM4-C-CE: 4-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3235 EM6-C-CE: 6-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3238 EM8-C-CE: 8-channel EasyMount system for tissue & cultured cells, CE (2.0 - 8.0 ml)
69-3241 EM2-C-HV-CE: 2-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3244 EM4-C-HV-CE: 4-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3247 EM6-C-HV-CE: 6-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3250 EM8-C-HV-CE: 8-channel EasyMount system for tissue & cultured cells, high voltage, CE (2.0 - 8.0 ml)
69-3231 EM2-R-CE: 2-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3234 EM4-R-CE: 4-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3237 EM6-R-CE: 6-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3240 EM8-R-CE: 8-channel EasyMount system for rat intestinal tissue, CE (2.0 - 8.0 ml)
69-3243 EM2-R-HV-CE: 2-channel EasyMount system for rat intestinal tissue, high voltage, CE (2.0 - 8.0 ml)
69-3249 EM6-R-HV-CE: 6-channel EasyMount system for rat intestinal tissue, high voltage, CE (2.0 - 8.0 ml)
69-3252 EM8-R-HV-CE: 8-channel EasyMount system for rat intestinal tissue, high voltage, CE (2.0 - 8.0 ml)
69-3230 EM2-LV-CE: 2-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3233 EM4-LV-CE: 4-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3236 EM6-LV-CE: 6-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3239 EM8-LV-CE: 8-channel EasyMount system for tissue & cultured cells, low volume, CE (0.5 - 5.0 ml)
69-3242 EM2-LV-HV-CE: 2-channel EasyMount system for tissue & cultured cells, low volume, high voltage, CE (0.5 - 5.0 ml)
69-3245 EM4-LV-HV-CE: 4-channel EasyMount system for tissue & cultured cells, low volume, high volt
69-3248 EM6-LV-HV-CE: 6-channel EasyMount system for tissue & cultured cells, low volume, high voltage, CE (0.5 - 5.0 ml)
69-0956 Insert for EasyMount Diffusion Chamber - for Snapwell Cell Culture
69-1081 (P2302M) EasyMount insert, fits P2300 chamber, for Millicell cell culture cups, 8.75 mm dia., 0.60 cm2
69-1082 (P2302N) EasyMount insert, fits P2300 chamber, for Nunc/Anopore cell culture cups, ,8.0 mm dia., 0.50 cm2
69-1083 (P2302T) EasyMount insert, fits P2300 chamber, for Costar Transwell cell culture cups, 6.5 mm dia, 0.33 cm2
69-1084 (P2303) EasyMount insert, fits P2300 chamber, for juvenile mouse intestine, 2.8x4.5 mm, 0.10 cm2
69-1085 (P2303A) EasyMount insert, fits P2300 chamber, with pins, for juvenile mouse intestine or bladder, 2.8x4.5 mm, 0.10 cm2
69-0957 Insert for EasyMount Diffusion Chamber - for Mouse Intestine
69-0958 Insert for EasyMount Diffusion Chamber - for Rat and Larger Animal Intestines
69-0959 Insert for EasyMount Diffusion Chamber - for Mouse Trachea, Similar Tissues
69-0960 Insert for EasyMount Diffusion Chamber - for Small Tissues (I.e., Biopsies)
69-1086 (P2308) EasyMount insert, fits P2300 chamber, for very small tissues (lower mouse trachea), 1 mm dia, 0.008 cm2
69-0961 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0962 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-1087 (P2311A) EasyMount insert, fits P2300 chamber, with mounting pins, 5.7 mm dia, 0.26 cm2
69-0963 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0964 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0965 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0966 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-1088 (P2316) EasyMount insert, fits P2300 chamber, with o-ring, for skin, 5.0 mm dia, 0.20 cm2
69-1089 (P2317) EasyMount insert, fits P2300 chamber, with o-ring, for synthetic membranes, 10.8 mm dia, 0.93 cm2
69-1090 (P2318) EasyMount insert, fits P2300 chamber, with o-ring, for frog skin, 11.3 mm dia, 1.00 cm2
69-1091 (P2319) EasyMount insert, fits P2300 chamber, with spacer ring, for studying mucus layer,12.7 mm dia, 1.26 cm2
69-1092 (P2403) EasyMount insert, fits P2400 chamber, for small tissue specimens, 2.8x4.5 mm, 0.10 cm2
69-0970 Insert for EasyMount Diffusion Chamber - for Rat and Larger Animal Intestines
69-0971 Insert for EasyMount Diffusion Chamber - for Mouse Trachea, Similar Tissues
69-0969 Insert for EasyMount Diffusion Chamber - for Mouse Intestine
69-1093 (P2408) EasyMount insert, fits P2400 chamber, for small tissues (biopsies), 0.8 mm dia, 0.005 cm2
69-1094 (P2408A) EasyMount insert, fits P2400 chamber, for small tissues (biopsies), 0.5 mm dia, 0.002 cm2
69-1095 (P2408B) EasyMount insert, fits P2400 chamber, for small tissues (biopsies), 1.0 mm dia, 0.008 cm2
69-0973 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0975 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-0976 Insert for EasyMount Diffusion Chamber - with Mounting Pins
69-1096 (P2300CAP) Chamber caps for P2300 chamber, set of 2
69-0990 Electrode Tips, pkg of 20
69-0991 Electrode Tips, pkg of 50
69-0992 Electrode Tips, pkg of 100
72-4924 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz
72-4921 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz
64-2244 (WB-KIT) TC-120 Thermocirculator Tubing Kit for EasyMount/Navicyte/Strathkelvin
69-0993 EasyMont Diffusion Chamber Model P2300
69-0995 EasyMont Diffusion Chamber Model P2400
69-0987 Voltage and Current Electrode Set
69-0988 Voltage Electrode Set
69-0989 Current Electrode Set
69-3147 (P2024-36) Electrode lead set, 36 in, 1 ea
69-0950 Single Channel Electrode Input Module and Dummy Membrane
69-0952 6-Channel Dummy Membrane for VCC MC6
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The VCC Amplifiers
The VCC-MC family of voltage/current clamps and accessories are designed as flexible tools for studying ion transport across epithelial tissue.

The VCC-MC family is of particular benefit in high throughput, multi-channel applications and where laboratory bench space is limited. All models provide similar capabilities and specifications to control voltage or te current across the epithelium. However, unlike the VCC-600, these clamps are modular in design.

VCC amplifiers now required the inclusion of an Optional Gain Control feature. Be sure to include this when you order. (See the Pricing & Ordering tab.)

The VCC-MC2
The VCC-MC2 contains two voltage/current clamp modules, a pulse generator, and a computer interface in a compact 9.5 inch chassis.

This amplifier is best suited for small scale applications and for student laboratories. It is compatible with all chamber systems offered by Warner. Each VCC-MC2 comes complete with two single channel input modules (DM-MC6).

The VCC Multichannel
The VCC-MC6 and VCC-MC8 are multichannel chassis that can be configured with 2-8 voltage/current clamp channels, depending on model and offer the advantage of expandability within a single instrument chassis. These instruments are ideally suited for experiments where multiple specimens are sampled in parallel.

A useful feature built into the VCC Multichannel instruments is a master control section. This section enables the investigator to quickly change the function, mode, and meter settings on all clamp modules from a single switch bank. This simplifies the use of the instrument and removes some of the tedium associated with continually having to change multiple identically placed switches on many channels.

Headstage    VCC-MCC Headstage
The VCC-MC family of amplifiers utilizes standard 2 mm pin-tip jacks for connection to electrode leads. Connections are facilitated by the use of input modules. The necessary modules are supplied with the VCC amplifiers, and each clamp channel/Ussing chamber required a unit. Input modules are compatible with both EasyMount and Harvard/Navicyte chambers.

The DM-MC6 is a single channel input module that can be mounted close to individual chambers. It connects to modular plugs on the rear of the VCC-MC instruments via a shielded cable. The module also contains an integral dummy membrane that is activated by an integral slide switch.

Dummy membranes    DM-600
Dummy membranes are useful both for quickly verifying the operating status of the instrument and as a learning aid for students.

The DM-660 is a single channel dummy membrane for use with the VCC-600. A similar dummy membrane is built into the DM-MC6 input module for the multi-channel clamps.

The DM-6 is a 6-channel dummy membrane for the VCC-MC6 and VCC-MC8 amplifiers. Each dummy membrane may be individually manipulated. The DM-6 connects to the VCC-MC6 and VCC-MC-8 via an EP-MC6 flat cable assembly. It runs on six AAA batteries

Specifications
Input Resistance > 109 Ω, differential
Common Mode Rejection > 100 dB
Frequency Response 4-stage adjustment via internal switch
Display: 3.5 digit, 3/8 in LCD with green backlight for each channel
Transepithelial
Voltage
±199.9 mV
Transepithelial
Current
±19.90, ±199.9, ±1999 µA, jumper selectable
Switches Push button, digital switching with LED indicators
Power 100/125 VAC, 60 Hz or 200/250 VAC, 50 Hz, user selectable
Meter Selects current or voltage to be displayed on panel meter
Function: Three position switch with LED indicator of state
Zero Standby mode, voltage inputs are internally grounded
Open Open circuit, voltage inputs are connected to sensing electrodes
Clamp Feedback loop is closed to control either current or voltage
Mode: Two switches select operating modes
Voltage/Current
(I/V)
Selects transepithelial voltage or current to be controlled when Function is on Clamp
Remote (REM) Selects control to be from front panel switches or from remote interface
Offset Potential ±10 or ±100 mV, set via precision 10-turn dial, jumper selectable ranges and lighted polarity switch
Fluid Resistance Push-button current injection, compensation set by precision 10-turn dial, jumper selectable ranges
On/Off 'On' sends pulse output to clamp, red indicator light
Polarity Selects +, - or bipolar pulses to be generated
Single Pulse Injects single pulse during interval between pulses
Reset Initializes interval timer and injects pulse
Amplitude 0 to 10 mV in 1 mV steps at X1 gain; 0 to 100 mV in 10 mV steps at X10 gain
Period 0.1 to 99.9 sec time period between pulses; set by 3-decade thumbwheel switch
Duration 0.01 to 9.99 sec duration of each pulse; set by 3-decade thumbwheel switch
DC Clamp Level: Sets DC holding voltage or current
Voltage Clamp ±100 mV via 10-turn dial, lighted polarity switch
Current Clamp Current gain dependent, 50% of max. displayed current
Master Override Permits control of function, mode, and meter features on all installed clamp channels
I/O Connection Specifications
Electrode Inputs 2 mm pin tip via DM-MC6 single channel input module, 1 per channel (VCC-MC6 and VCC-MC8) 2 mm pin tip via electrode panel (EP-MC6) for Harvard/Navicyte chamber system (VCC-MC6)
Recorder Output Voltage 10 mV/mV, x10 transepithelial voltage
Recorder Output Current Jumper selectable, 1, 10 or 100 mV/µA
Pulse Generator 0 to ±100 mV and 0 to ±1 V
External Input Allows arbitrary analog input signal to be clamped
Remote Interface 9-pin DB connector, 1 per channel (VCC-MC2)25-pin DB connector (all others)
  1. Overexpression of claudin-7 decreases the paracellular Cl– conductance and increases the paracellular Na+ conductance in LLC-PK1 cells
    Alexandre MD, Lu Q, Chen YH
    J Cell Sci. 2005 Jun 15;118(Pt 12):2683-93
  2. Expression and function of bradykinin B1 and B2 receptors in normal and inflamed rat urinary bladder urothelium
    Chopra B, Barrick SR, Meyers S, Beckel JM, Zeidel ML, Ford AP, de Groat WC, Birder LA
    J Physiol. 2005 Feb 1;562(Pt 3):859-71
DOCUMENT DESCRIPTION
Warner's Guide to Ussing Chamber Systems (091022) WLR.pdfWarner's Guide to Ussing Chamber Systems