Mitocell Respirometry System (MS-200, MS-200A)

Mitocell Respirometry System (MS-200, MS-200A)

The Mitocell Respirometry System is a complete package designed to accommodate automatic oxidative phosphorylation measurements in both isolated mitochondria and intact cells.

Item# Description U.S. List Price Quantity
69-0034 (MS-200) Mitocell Precision Respirometry System, 50-100 ul
69-0035 (MS-200A) Mitocell Precision Respirometry System, 300-1000 ul
72-6187 Replacement Glass Plunger for MT200
69-3017 (SI-066) Modified syringe for MT-200 and MS-200, 1
69-3038 (SI-058) Plunger for MT-200 and MS-200
69-3040 (SI070) Adjustment rod and hex key for MT-200 and MS-200
98-2200 (SI-069) Spinbars for MT-200 and MS-200, 6 pack, with magnet
72-6188 Replacement Glass Plunger for MT200A
69-3015 (SI-067) Modified syringe for MT-200A and MS-200A, 5
69-3037 (SI-056) Plunger for MT-200A and MS-200A
69-3039 (SI-068) Spinbars for MT-200A and MS-200A, 6 pack
69-3041 (SI071) Volume peices and hex key for MT-200A and MS-200A
69-3033 Mitocell Miniature Respirometer MT 200, 50/100 µl
69-3018 Respiration Cell RC 300, 0.31 ml
69-3019 Respiration Cell RC 350, 1.0 - 3.0 ml
69-3016 MicroCell Model MC 100
69-3023 Flow Cell Model FC 100
72-4924 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz
72-4921 (TC-120) Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz
64-2244 (WB-KIT) TC-120 Thermocirculator Tubing Kit for EasyMount/Navicyte/Strathkelvin
69-3007 Oxygen Electrode Service Kit
69-3005 (SI-021) FEP oxygen electrode service kit
69-3008 Electrolyte Solution, 125 ml
69-3013 O-Rings, Box of 25 SI130
69-3014 (SI-045) Polyproplyene membraned jackets, box of 6
69-3034 (SI-023) Polyproplyene membraned jackets, box of 30
69-3010 FEP Membranes, pkg. of 15
69-3011 Electrode Jacket (with Hole) for FEP Membranes
69-3036 (SI-043) O-rings for FEP membranes, 25 pack
69-3035 (SI-031) Abrasive membranes, box of 6
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The MS-200 and MS-200A Mitocell Respirometry System is the system of choice if you need to make oxidative phosphorylation measurements in either isolated mitochondria or intact cells.

The two systems offered are identical with the exception of the included Mitocell. The MS-200 incorporates the low volume MT-200 Mitocell, and the MS-200A incorporates the larger volume MT-200A Mitocell. The MT-200 has working volumes of 50 and 100 ul, while the MT-200A has working volumes of 300, 500, and 1000 ul.

The special software powers a dedicated platform for mitochondrial research and will calculate State 3 and State 4 respiration rates, P/O ratios and RCR. The program also records and automatically calculates general respiration rates and specific oxygen uptake rates.

    Applications include:
  • Mitochondrial dysfunction characterization
  • Cytotoxicology research with cultured cells
  • Respiration of permeabilised liver tissue and muscle fibers
  • Bactericidal cytotoxicity testing
  • Cell respiration in apoptosis, oxidative stress, and inflammation
  • Oxygen uptake in isolated enzyme systems and sub-mitochondrial particles

The Mitocell MS-200 includes a 782 oxygen meter, a 1302 oxygen electrode, an MT-200 or MT-200A respirometer, and special respirometry software specifically designed for making oxidative phosphorylation measurements. The system also includes an electrode service kit and an easy-to-follow manual.

Note: You have the option to purchase a second Mitocell and 1302 electrode to take advantage of the capacity of the 782 meter and software to record from 2 electrodes simultaneously.


Specifications for MT-200 and MT-200A

Sample chamber 50 µl, 100 µl 300 µl, 500 µl, 1000 µl
Diameter 4 mm 4 mm
Chamber unit Glass Glass
Plunger Polycarbonate Polycarbonate
Bore 1 mm 1 mm
Magnetic stirrer 5 volt DC from plug-top power supply 5 volt DC from plug-top power supply
Shipping Dimensions 12 x 12 x 12 inches 12 x 12 x 12 inches
Shipping Weight 8lbs 8lbs


Specifications for 782 Oxygen Sensor

Resolution with 1302 electrode 0.1% of full scale
Logging Capacity 2000 readings
Off-line data storage 4 hours at 1/sec reading
PC requirements Windows PC, USB port, with Windows 7 64 bit or earlier;
4 MB of hard disk space and at least 64 MB of RAM
Power 5 VDC; 1 A, w/external plug
Power supply 100-230 VAC, 47-63 Hz
Dimensions 185 x 135 x 105 mm (7.25 x 5.25 x 4.125 in)


Specifications for Oxygen Electrode 1302

Response time at 37°C:  
Polypropylene membranes 18 sec for 90% change
FEP membranes 6 sec for 90% change
Oxygen consumption (polypropylene membranes) 0.5 to 3x10-10mg O2/min
Temperature coefficient 2% per °C
Resolution of 928 and 782 meter 0.1% with 1302 electrode*

*The display resolution of the 982 and 782 meter is 0.1%. The A/D converter is capable of at least 0.01%, so you can work on an expanded scale. However, the limit is normally set to about 0.1% by the noise level of the 1302 electrode.