PU MP R EFER ENC ES Common Syringe Data Diameter and Plunger Surface Area The following list is a guide to common syringes and their associated diameters and surface area. Syringe diameter data, in mm, is listed below for each syringe. All Harvard Apparatus microprocessor syringe pumps require the user to input syringe diameter information. The pump uses this diameter data to set flow rates. The PHD 22/2000 series of syringe pumps also has this information built into the pump memory in a handy Syringe Look Up Table. Surface area information was used to calculate PSI (pounds per square inch) data for the pressure on page 111. Average pressures for any syringe pump and syringe combination can be calculated by dividing the average (nominal) syringe pump force by the syringe diameter (in square inches) to obtain PSI. Example, nominal pressure obtained using a 25 ml Hamilton Gastight® Syringe on a PHD 22/2000 standard pressure syringe pump would be: 50 lbs / 0.644 in2 = 77.6 PSI (5.35 bars). PUMP REFERENCES Common Syringe Data 114 Common Syringe Diameters Volume BD Plastic 1 ml 3 ml 5 ml 10 ml 20 ml 30 ml 50/60 ml BD Glass 0.5 ml 1 ml 2.5 ml 5 ml 10 ml 20 ml 30 ml 50 ml 100 ml SGE Glass 25 µl 50 µl 100 µl 250 µl 500 µl 1 ml 2.5 ml 5 ml 10 ml 0.73 1.03 1.46 2.3 3.26 4.61 7.28 10.3 14.57 0.000649 0.001292 0.002595 0.006440 0.012938 0.025872 0.064519 0.129151 0.258429 4.64 4.64 8.66 11.86 14.34 19.13 22.7 28.6 34.9 0.026209 0.026209 0.091297 0.171235 0.250335 0.445505 0.627298 0.995760 1.482768 4.699 8.585 11.989 14.427 19.05 21.59 26.594 0.026880 0.089722 0.174980 0.253381 0.441786 0.567450 0.860974 Diameter (mm) Surface Area (in2) Common Syringe Diameters Volume Diameter (mm) Surface Area (in2) Common Syringe Diameters Volume Diameter (mm) Surface Area (in2) Harvard Apparatus Stainless Steel 2.5 ml 8 ml 20 ml 50 ml 100 ml 200 ml Terumo Plastic 3 ml 5 ml 10 ml 20 ml 30 ml 60 ml 8.95 13 15.8 20.15 23.1 29.1 0.097514 0.205735 0.303904 0.494279 0.649601 1.030881 4.851 9.525 19.13 28.6 34.9 44.75 0.027937 0.110447 0.445505 0.995760 1.482768 2.438382 Hamilton Gastight Glass 0.5 µl 1 µl 2 µl 5 µl 10 µl 25 µl 50 µl 100 µl 250 µl 500 µl 1 ml 2.5 ml 5 ml 10 ml 0.103 0.146 0.206 0.343 0.485 0.729 1.03 1.457 2.304 3.256 4.608 7.285 10.3 14.567 23.033 32.573 32.573 0.000013 0.000026 0.000052 0.000129 0.000258 0.000647 0.001294 0.002595 0.006440 0.012938 0.025872 0.064519 0.129151 0.258429 0.643989 1.293772 1.293772 Air-Tite All Plastic 2.5 ml 5 ml 10 ml 20 ml 30 ml 50 ml 9.6 12.45 15.9 20.05 22.9 29.2 0.112193 0.188695 0.307763 0.489386 0.638401 1.037979 25 ml 50 ml 100 ml Covidien Monoject Plastic (formerly Kendall) 1 ml 3 ml 6 ml 12 ml 20 ml 35 ml 60 ml 140 ml 4.674 8.865 12.600 15.621 20.142 23.571 26.568 37.948 0.026323 0.097297 0.196350 0.307763 0.506621 0.689567 0.861362 1.795084 Cadence Science (formerly Popper & Sons) Perfectum Glass 0.5 ml 1 ml 2 ml 3 ml 5 ml 10 ml 20 ml 30 ml 50 ml 100 ml 3.45 4.5 8.92 8.99 11.7 14.7 19.58 22.7 29 35.7 0.014490 0.024652 0.096862 0.098388 0.166646 0.263061 0.466711 0.627298 1.023808 1.551525 Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com