P UMP R EFER ENC ES Syringe Pump Pressure and Flow Rate Minimum/Maximum Flow Rates By Pump and Syringe Size PHD ULTRA™ Flow Rates Nominal Minimum/Maximum Flow Rates for Various Syringes. (Actual Limits will vary depending on syringe manufacturer) Syringe Size 0.5 µl 1 µl 2 µl 5 µl 10 µl 25 µl 50 µl 100 µl 250 µl 500 µl 1 ml 2.5 ml 3 ml 5 ml 8 ml 10 ml 20 ml 30 ml 50 ml 100 ml 140 ml Diameter * 0.103 mm 0.1457 mm 0.206 mm 0.343 mm 0.485 mm 0.729 mm 1.030 mm 1.457 mm 2.304 mm 3.256 mm 4.699 mm 4.851 mm 8.585 mm 11.989 mm 9.525 mm 14.427 mm 19.050 mm 21.590 mm 26.594 mm 35.700 mm 37.948 mm Minimum 1.500 pl/min 3.060 pl/min 6.120 pl/min 16.980 pl/min 33.960 pl/min 76.740 pl/min 153.180 pl/min 306.600 pl/min 766.740 pl/min 1.53126 nl/min 3.18936 nl/min 3.3990 nl/min 10.645 nl/min 20.7616 nl/min 13.1046 nl/min 30.0640 nl/min 52.4186 nl/min 67.3288 nl/min 102.156 nl/min 184.091 nl/min 208.005 nl/min Maximum 1.59133 µl/min 3.18423 µl/min 6.36532 µl/min 17.6471 µl/min 35.2833 µl/min 79.7151 µl/min 159.133 µl/min 318.423 µl/min 796.252 µl/min 1.59021 ml/min 3.31205 ml/min 3.52979 ml/min 11.0552 ml/min 21.5601 ml/min 13.6087 ml/min 31.2204 ml/min 54.4347 ml/min 69.9183 ml/min 106.085 ml/min 191.171 ml/min 216.005 ml/min PUMP REFERENCES PHD 22/2000 and PHD 4400 Hpsi Flow Rates Syringe Size 0.5 µl 1 µl 2 µl 5 µl 10 µl 25 µl 50 µl 100 µl 250 µl 500 µl 1000 µl 1 ml 2 ml 2.5 ml 3 ml 5 ml 10 ml 20 ml 30 ml 50 ml 100 ml 140 ml Diameter * 0.10 mm 0.15 mm 0.21 mm 0.33 mm 0.46 mm 0.73 mm 1.03 mm 1.46 mm 2.30 mm 3.26 mm 4.61 mm 5.00 mm 9.00 mm 7.28 to 9.6 mm 8.66 to 9.0 mm 10.3 to 13.0 mm 14.57 to 15.9 mm 21.7 to 23.2 mm 26.7 to 32.6 mm 34.9 to 35.7 mm 38.40 mm Minimum 0.0001 µl/hr 0.0002 µl/hr 0.0004 µl/hr 0.0010 µl/hr 0.0019 µl/hr 0.0046 µl/hr 0.0092 µl/hr 0.0183 µl/hr 0.0454 µl/hr 0.0911 µl/hr 0.0031 µl/min 0.0033 µl/min 0.0119 µl/min 0.0076 µl/min 0.0100 µl/min 0.0208 µl/min 0.0301 µl/min 0.0673 µl/min 0.1019 µl/min 0.1740 µl/min 0.2106 µl/min Maximum 95.330 µl/hr 190.70 µl/hr 381.30 µl/hr 953.17 µl/hr 1.901 ml/hr 4.775 ml/hr 9.551 ml/hr 19.153 ml/hr 47.532 ml/hr 95.492 ml/hr 190.950 ml/hr 205.30 ml/hr 747.35 ml/hr 476.21 ml/hr 11.231 ml/min 21.781 ml/min 31.486 ml/min 54.804 ml/min 70.518 ml/min 106.76 ml/min 182.40 ml/min 220.82 ml/min Syringe Pump Pressure and Flow Rate 19.13 to 20.05 mm 0.0523 µl/min * Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart on page 114 * Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart on page 114. Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com 113