P UMP R EFER ENC ES Syringe Pump Pressure and Flow Rate How to Calculate the Pressure of Various Syringe Sizes The pressure that a syringe pump can generate is a function of both the force of the pump (measured at the pusher block in pounds) as well as the physical characteristics of the syringe and setup used. The following table compares various syringe pumps and the pressures in PSI (pounds per square inch). Each data point was calculated by dividing the average pump force by the surface area (in square inches) of syringes with diameters from 0.1 to 50 mm. Diameters and surface areas for a variety of syringes can be found in the table on page 114. This table is intended to be a guide of total pressures generated. Actual values may be higher or lower than the listed pressures due to the influence of other factors such as tubing diameter and length. When using more than one syringe sharing the same pusher block, the pressure is calculated by dividing the force (lbs) by the total surface area (square inches) of all syringes on the pump. For example, nominal pressure obtained using two 25 ml Hamilton Gastight® syringes on a PHD 22/2000 standard pressure syringe pump would be: 50 lbs / (0.644 in2 X 2) = 38.81 PSI (2.68 bars). PUMP REFERENCES How to Calculate the Pressure Requirement of Your Experiment Minimum/Maximum Flow Rates by Pump and Syringe Size Flow rates were calculated based on the pusher block travel rate for each pump (rate at which the syringe pump moves the syringe plunger) and the diameter of the syringe. PHD 22/2000 HPSI Flow Rates Syringe Size 20 ml 50 ml 100 ml 200 ml Diameter ** 19.13 mm 28.60 mm 34.90 mm 44.75 mm Minimum 1.5 µl/hr 3.4 µl/hr 5.0 µl/hr 8.2 µl/hr Maximum 20 ml/min 46 ml/min 68 ml/min 112 ml/min Pump 11 Elite Flow Rates Nominal Minimum/Maximum Flow Rates for Various Syringes. (Actual Limits will vary depending on syringe manufacturer) Syringe Size 0.5 µl 1 µl 2 µl 5 µl 10 µl 25 µl Diameter * 0.103 mm 0.146 mm 0.206 mm 0.343 mm 0.485 mm 0.729 mm 1.030 mm 1.457 mm 2.304 mm 3.256 mm 4.608 mm 4.699 mm 8.585 mm 11.99 mm 14.43 mm 19.05 mm 21.59 mm 26.59 mm 26.59 mm Minimum 1.26 pl/min 2.52 pl/min 5.10 pl/min 14.160 pl/min 28.260 pl/min 63.960 pl/min 127.700 pl/min 255.500 pl/min 638.900 pl/min 1.276 nl/min 2.556 nl/min 2.658 nl/min 8.871 nl/min 17.300 nl/min 25.050 nl/min 43.680 nl/min 56.110 nl/min 85.130 nl/min 85.130 nl/min Maximum 1.326 µl/min 2.654 µl/min 5.304 µl/min 14.710 µl/min 29.400 µl/min 66.430 µl/min 132.600 µl/min 265.400 µl/min 663.500 µl/min 1.325 ml/min 2.654 ml/min 2.760 ml/min 9.213 ml/min 17.970 ml/min 26.020 ml/min 45.360 ml/min 58.270 ml/min 88.400 ml/min 88.400 ml/min * The Rates listed are for single stainless steel syringe **Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart on page 114. PHD ULTRA™ HPSI & XF Flow Rates Syringe Size 20 ml 30 ml 50 ml 100 ml 130 ml 200 ml Syringe ID ** 19.130 mm 21.590 mm 28.600 mm 34.900 mm 37.948 mm 44.755 mm Minimum Rate 50.7884 nl/min 64.6904 nl/min 113.519 nl/min 169.038 nl/min 199.854 nl/min 277.983 nl/min Maximum Rate 26.3709 ml/min 33.5893 ml/min 58.9423 ml/min 87.7700 ml/min 103.770 ml/min 144.337 ml/min 50 µl 100 µl 250 µl 500 µl 1000 µl 1 ml 3 ml 5 ml 10 ml 20 ml 30 ml 50 ml 60 ml * The Rates listed are for single stainless steel syringe **Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart on page 114. * Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart on page 114 Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com 111