Syringe Pumps Application Selection Guide Cell Biology Cellular studies typically involve the injection of very small volumes under controlled conditions. Several models offer a foot pedal accessory to start/stop flow on command. • For cellular microinjections of plasmids, viruses, and the like, see either the Pump 11 Pico Plus Elite on page 14, PHD ULTRA™ Nanomite on page 28 or the Pump 11 Elite Nanomite on page 16. Mass Spectrometry Calibration and Electrospray Ionization Calibration and Electrospray Ionization Where precise volume delivery and pulse free flow are required for optimum instrument calibration or solvent elimination, the Pump 11 series has long been the industry standard for manufacturers in OEM configurations and end users. See the Pump 11 Elite on page 11, and Pump 11 Pico Plus Elite systems on page 14. Additionally, use of the gradient capabilities of the Pump 11 Elite or PHD ULTRA™ Series allows for automated multi-point calibration when performing GLP studies. SYRINGE PUMPS Application Selection Guide MALDI-TOF Matrix Addition Using the Nanomite with its detachable delivery and electronics allows for easy placement of the syringe over the target to facilitate the dispensing of the appropriate matrix. See the PHD ULTRA™ Nanomite on page 28 or the Pump 11 Elite Nanomite on page 16. Cell Biology References Microfluidic electroporation for selective release of intracellular molecules at the single-cell level, ELECTROPHORESIS, Volume 29, Issue 14, pages 2939–2944, No. 14 July 2008 L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear conditions, THE JOURNAL OF BIOLOGICAL CHEMISTRY, 283, 15816-15824 Shear assay measurements of cell adhesion on biomaterials surfaces, MATERIALS SCIENCE AND ENGINEERING: C, Volume 29, Issue 4, 5 May 2009, Pages 1293-1301 Chemical & Industrial/Electrospinning Where chemical synthesis, pressurized systems, and other applications demand high performance Harvard Apparatus has the right solution. • For high pressure applications, consider the PHD ULTRA™ XF and PHD ULTRA™ HPSI with stainless steel syringes on page 26 or the PHD ULTRA™ 4400 on page 27. Harvard Apparatus pumps are used in creating Nano and Micro scale polymer fibers in a high voltage field. The stability of flow and flexibility of use, in the PHD ULTRA™ series pumps, makes it easy to alter the variables relating to successful electrospinning. Our pumps include a grounded power supply and can be supplied with a grounding strap, which will shunt any high voltage arcs to ground. Mass Spectrometry Calibration References Which Electrospray-Based Ionization Method Best Reflects Protein-Ligand Interactions Found in Solution? A Comparison of ESI, nanoESI, and ESSI for the Determination of Dissociation Constants with Mass Spectrometry, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,Volume 19, Issue 3, March 2008, Pages 332-343 Gas-phase IR spectra of intact α-helical coiled coil protein complexes, International Journal of Mass Spectrometry, INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, Volume 283, Issues 1-3, 1 June 2009, Pages 161-168 Fragmentation patterns of newly isolated cassane butenolide diterpenes and differentiation of stereoisomer by tandem mass spectrometry, JOURNAL OF MASS SPECTROMETRY, Volume 43, Issue 10, pages 1413–1420, October 2008 Chemical and Industrial/Electrospinning References Microfluidic electroporation for selective release of intracellular molecules at the single-cell level, ANAL. CHEM., 2008, 80 (9), pp 3112–3122 Optical and electrical properties of indium tin oxide nanofibers prepared by electrospinning, NANOTECHNOLOGY, 2008 19 145603 Manufacture of small calibre quadruple lamina vascular bypass grafts using a novel automated extrusion-phase-inversion method and nanocomposite polymer, JOURNAL OF BIOMECHANICS, Vol. 42, ssue 6, Pages 722-730 (16 April 2009) Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com 9