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Harvard Apparatus
phone
508.893.8999
toll free U.S.
800.272.2775
fax
508.429.5732
www.harvardapparatus.com
112
PUMP REFERENCES
Common Syringe Data
Harvard Apparatus
phone
508.893.8999
toll free U.S.
800.272.2775
fax
508.429.5732
www.harvardapparatus.com
The following list is a guide to common syringes and their associated
diameters and surface area. Syringe diameter data, in mm, is listed
below for each syringe. All Harvard Apparatus microprocessor syringe
pumps require the user to input syringe diameter information. The
pump uses this diameter data to set flow rates. The PHD 22/2000
series of syringe pumps also has this information built into the pump
memory in a handy Syringe Look Up Table. Surface area information
was used to calculate PSI (pounds per square inch) data for the
pressure on page 111. Average pressures for any syringe pump
and syringe combination can be calculated by dividing the average
(nominal) syringe pump force by the syringe diameter (in square
inches) to obtain PSI. Example, nominal pressure obtained using a 25
ml Hamilton Gastight
®
Syringe on a PHD 22/2000 standard pressure
syringe pump would be: 50 lbs / 0.644 in2 = 77.6 PSI (5.35 bars).
Diameter and Plunger Surface Area
Common Syringe
Diameters

Volume
Diameter
(mm)
Surface
Area (in
2
)
BD Plastic
1 ml
4.699
0.026880
3 ml
8.585
0.089722
5 ml
11.989
0.174980
10 ml
14.427
0.253381
20 ml
19.05
0.441786
30 ml
21.59
0.567450
50/60 ml
26.594
0.860974
BD Glass
0.5 ml
4.64
0.026209
1 ml
4.64
0.026209
2.5 ml
8.66
0.091297
5 ml
11.86
0.171235
10 ml
14.34
0.250335
20 ml
19.13
0.445505
30 ml
22.7
0.627298
50 ml
28.6
0.995760
100 ml
34.9
1.482768
SGE Glass
25 µl
0.73
0.000649
50 µl
1.03
0.001292
100 µl
1.46
0.002595
250 µl
2.3
0.006440
500 µl
3.26
0.012938
1 ml
4.61
0.025872
2.5 ml
7.28
0.064519
5 ml
10.3
0.129151
10 ml
14.57
0.258429
Common Syringe
Diameters

Volume
Diameter
(mm)
Surface
Area (in
2
)
Harvard Apparatus Stainless Steel
2.5 ml
4.851
0.027937
8 ml
9.525
0.110447
20 ml
19.13
0.445505
50 ml
28.6
0.995760
100 ml
34.9
1.482768
200 ml
44.75
2.438382
Terumo Plastic
3 ml
8.95
0.097514
5 ml
13
0.205735
10 ml
15.8
0.303904
20 ml
20.15
0.494279
30 ml
23.1
0.649601
60 ml
29.1
1.030881
Air-Tite All Plastic
2.5 ml
9.6
0.112193
5 ml
12.45
0.188695
10 ml
15.9
0.307763
20 ml
20.05
0.489386
30 ml
22.9
0.638401
50 ml
29.2
1.037979
Cadence Science (formerly Popper & Sons)
Perfectum Glass
0.5 ml
3.45
0.014490
1 ml
4.5
0.024652
2 ml
8.92
0.096862
3 ml
8.99
0.098388
5 ml
11.7
0.166646
10 ml
14.7
0.263061
20 ml
19.58
0.466711
30 ml
22.7
0.627298
50 ml
29
1.023808
100 ml
35.7
1.551525
Common Syringe
Diameters

Volume
Diameter
(mm)
Surface
Area (in
2
)
Hamilton Gastight Glass
0.5 µl
0.103
0.000013
1 µl
0.146
0.000026
2 µl
0.206
0.000052
5 µl
0.343
0.000129
10 µl
0.485
0.000258
25 µl
0.729
0.000647
50 µl
1.03
0.001294
100 µl
1.457
0.002595
250 µl
2.304
0.006440
500 µl
3.256
0.012938
1 ml
4.608
0.025872
2.5 ml
7.285
0.064519
5 ml
10.3
0.129151
10 ml
14.567
0.258429
25 ml
23.033
0.643989
50 ml
32.573
1.293772
100 ml
32.573
1.293772
Covidien Monoject Plastic (formerly Kendall)
1 ml
4.674
0.026323
3 ml
8.865
0.097297
6 ml
12.600
0.196350
12 ml
15.621
0.307763
20 ml
20.142
0.506621
35 ml
23.571
0.689567
60 ml
26.568
0.861362
140 ml
37.948
1.795084
PUMP REFERENCES
Common Syringe Data