Harvard Apparatus
phone
508.893.8999
toll free U.S.
800.272.2775
fax
508.429.5732
www.harvardapparatus.com
108
Harvard Apparatus
phone
508.893.8999
toll free U.S.
800.272.2775
fax
508.429.5732
www.harvardapparatus.com
NORMAL PRESSURE
0 to 30 psi
(0 to 2 bar)
HIGH FORCE
31 to 150 psi
(2.1 to 10.2 bar)
EXTREME HIGH PRESSURE
151 to 2000 psi
(10.3 to 137 bar)
1.
Application
L
O
W
Flow into open containers,
i.e. titrations, food trays filling
Inject into Tissue,
i.e. Drug infusion into muscle, brain
H
I
G
H
Flow into closed container,
i.e. Reaction Chamber 350 to 400 psi
High viscosity solutions at high flow rates in a short period of time,
i.e. Corn syrup
2.
FLOW RATE - Pumping Speed (The faster the flow rate, the higher the pressure)
0.003 µl/hr to 140 ml/min
141 ml/min to 220 ml/min
3.
SYRINGE SIZE (Syringe volume/plunger area + linear force of pump)
10 µl to 1 ml ie. 500 µl/min x 20 lbs = 1500 psi
4.
TUBING SIZE (Inner diameter, Smaller ID = higher pressure)
SMALL - Capillary (the longer more pressure)
LARGE - Hose
5.
TUBING LENGTH - DISTANCE (Depends on ID Smaller ID = higher pressure)
Short, < 1M
Long, > 1M
6.
VISCOSITY OF MATERIAL TO BE PUMPED (Higher viscosity = higher pressure)
AIR
18°C = 0.0182 cP
WATER
20°C = 1.002 cP
OLIVE OIL
20°C = 84 cP
PANCAKE SYRUP 20°C = 2500 cP
HONEY
20°C = 10000 cP
PEANUT BUTTER 20°C = 250000 cP
7.
TEMPERATURE OF SOLUTIONS BEING PUMPED (Higher temperature = lower viscosity = lower pressure)
0 to 15°C
15 to 80°C
1
Nature of the sample you are flowing into (Application)
2
The flow rate of the material
3
The surface area of the syringe
and the linear force capability of the pump
4
The tubing diameter
5
The tubing length
6
Viscosity of the material being pumped
7
The temperature of the material being pumped
PUMP REFERENCES
How to Calculate the
Pressure Requirement of Your Experiment
T
he following chart will help you determine the pressure requirement of your experiment.
This is important in selecting the correct pump with the proper psi capability for your
application. Choose the selections that are the closest to your experimental conditions or write in
your actual values. Once you have filled in the chart call us for technical assistance if needed.
PUMP REFERENCES
How to Calculate the Pressure Requirement of Your Experiment