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Harvard Apparatus
phone
508.893.8999
toll free U.S.
800.272.2775
fax
508.429.5732
www.harvardapparatus.com
SYRINGE PUMPS
Application Selection Guide
9
Cell Biology
Cellular studies typically involve the injection of very small volumes
under controlled conditions. Several models offer a foot pedal
accessory to start/stop flow on command.
·
For cellular microinjections of plasmids, viruses, and the
like,
see either the Pump 11 Pico Plus Elite on page 14,
PHD ULTRA
TM
Nanomite on page 28 or the Pump 11 Elite
Nanomite on page 16.
Chemical & Industrial/Electrospinning
Where chemical synthesis, pressurized systems, and other applications
demand high performance Harvard Apparatus has the right solution.
·
For high pressure applications, consider the PHD ULTRA
TM
XF and PHD ULTRA
TM
HPSI with stainless steel syringes on
page 26 or the
PHD ULTRA
TM
4400
on page 27.
Harvard Apparatus pumps are
used in creating Nano and Micro
scale polymer fibers in a high
voltage field. The stability of flow
and flexibility of use, in the PHD
ULTRA
TM
series pumps, makes it easy to alter the variables relating
to successful electrospinning. Our pumps include a grounded power
supply and can be supplied with a grounding strap, which will shunt
any high voltage arcs to ground.
Mass Spectrometry Calibration
and Electrospray Ionization
Calibration and Electrospray Ionization
Where precise volume delivery and pulse free flow are required for
optimum instrument calibration or solvent elimination, the Pump 11
series has long been the industry standard for manufacturers in OEM
configurations and end users. See the Pump 11 Elite on page 11, and
Pump 11 Pico Plus Elite systems on page 14.
Additionally, use of the gradient capabilities of the Pump 11 Elite
or PHD ULTRA
TM
Series allows for automated multi-point calibration
when performing GLP studies.
MALDI-TOF Matrix Addition
Using the Nanomite with its detachable delivery and electronics
allows for easy placement of the syringe over the target to
facilitate the dispensing of the
appropriate matrix. See the
PHD ULTRA
TM
Nanomite
on page 28 or the
Pump 11 Elite
Nanomite on
page 16.
Cell Biology
References
Microfluidic electroporation for selective release of intracellular molecules at the
single-cell level,
ELECTROPHORESIS, Volume 29, Issue 14, pages 2939­2944,
No. 14 July 2008
L-selectin-mediated lymphocyte-cancer cell interactions under low fluid shear
conditions,
THE JOURNAL OF BIOLOGICAL CHEMISTRY, 283, 15816-15824
Shear assay measurements of cell adhesion on biomaterials surfaces,
MATERIALS SCIENCE AND ENGINEERING: C, Volume 29, Issue 4, 5 May 2009,
Pages 1293-1301
Chemical and Industrial/Electrospinning
References
Microfluidic electroporation for selective release of intracellular molecules at the
single-cell level,
ANAL. CHEM., 2008, 80 (9), pp 3112­3122
Optical and electrical properties of indium tin oxide nanofibers prepared by

electrospinning,
NANOTECHNOLOGY, 2008 19 145603
Manufacture of small calibre quadruple lamina vascular bypass grafts using a
novel automated extrusion-phase-inversion method and nanocomposite polymer,
JOURNAL OF BIOMECHANICS, Vol. 42, ssue 6, Pages 722-730 (16 April 2009)
Mass Spectrometry Calibration
References
Which Electrospray-Based Ionization Method Best Reflects Protein-Ligand
Interactions Found in Solution? A Comparison of ESI, nanoESI, and ESSI for the
Determination of Dissociation Constants with Mass Spectrometry,
JOURNAL OF
THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,Volume 19, Issue 3,
March 2008, Pages 332-343
Gas-phase IR spectra of intact
-helical coiled coil protein complexes,
International Journal of Mass Spectrometry,
INTERNATIONAL JOURNAL OF MASS
SPECTROMETRY, Volume 283, Issues 1-3, 1 June 2009, Pages 161-168
Fragmentation patterns of newly isolated cassane butenolide diterpenes and
differentiation of stereoisomer by tandem mass spectrometry,
JOURNAL OF MASS
SPECTROMETRY, Volume 43, Issue 10, pages 1413­1420, October 2008
Application Selection Guide
SYRINGE PUMPS