Low Profile Parallel Plate Flow Chamber (RC-31)

Low Profile Parallel Plate Flow Chamber (RC-31)

The RC-31 is a closed bath, low profile chamber incorporating special features for microscopic confocal imaging.

  • Adjustable distance between upper and lower coverslips, 50 µm to 350 µm
  • Optimal control of culture conditions and cellular environment
  • Compatible with both upright and inverted microscopes
  • Amenable for long term studies using live cells
  • Uses Warner's Series 30 stage adapters

Item# Description U.S. List Price Quantity
64-0754 (PE-90/10) Polyethylene tubing, PE-90, 10 ft
64-1696 (CS-40R15) Round cover glass, #1.5 thickness, 40 mm, 50 pack
64-1499 (CS-30R15) Round cover glass, #1.5 thickness, 30 mm, 90 pack
64-0335 Replacement Gaskets for Blank, 375 um Thickness, pkg of 10
64-0334 Replacement Gaskets for Blank, 250 um Thickness, pkg of 10
64-0341 Replacement Gaskets for Blanks, 150 um Thickness, pkg of 10
64-0333 Replacement Gaskets for Slotted Bath, 375 um Thickness, pkg of 10
64-0332 Replacement Gaskets for Slotted Bath, 250 um Thickness, pkg of 10
64-0343 Replacement Gaskets for Slotted Bath, 150 um Thickness, pkg of 10
64-0331 Replacement Gaskets for Large Bath, 375 um Thickness, pkg of 10
64-0330 Replacement Gaskets for Large Bath, 250 um Thickness, pkg of 10
64-0342 Replacement Gaskets for Large Bath, 150 um Thickness, pkg of 10
64-1685 Confocal imaging chamber (uses Series 30 platforms)
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Unique features of the RC-31 include user defined bath geometry and a large viewing area. A 30 mm, #1.5 glass coverslip forms the top of the chamber, while a 40 mm, #1.5 coverslip forms the bottom, respectively, creating a closed bath. The closed design allows the chamber to be inverted in the mounting platform enabling its use with both inverted or upright microscopes. The final viewing aperture is 25 mm diameter.

Variable Bath Volume/Fast Perfusate Exchange

The side walls of the RC-31 bath is formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 50-350 µm and is defined by the thickness and number of gaskets used.

Gaskets

Pre-cut gaskets having two different geometries are also supplied; one having a wide cut to allow full use of the 17.7 mm diameter aperture window, and a narrow, slotted version (4 x 37 mm) designed for fast solution exchange. Blank gaskets are also supplied with the chamber permitting the development of the customized bath geometry required for individual applications.

 

The RC-31 comes complete with 30 mm round top coverglass, 40 mm round bottom coverglass, blank gaskets (10 pk each in 150, 250, and 375 µm thickness), slotted gaskets (10 pk each in 150, 250, and 375 µm thickness), and wide gaskets (10 pk each in 150, 250, and 375 µm thickness), and 10 ft PE-90 Tubing

Specifications64-034164-034364-034264-1685
ModelGS-30B/4GS-30S/4GS-30L/4RC-31
Physical Di mensions150 um150 um150 um11.5 x 82 mm
VolumeFor 250um thick gaskets:
Slotted bath 26
Physical Dimensions (H X D) 11.5 x 82 mm
Viewing Area 23 mm in diameter
Bath volumes For 250um thick gaskets:
Slotted bath 26 µl
Wide bath 133 µl
Weight 0.22 kg, assembled with optional heaters
Construction Aluminum, black anodized
Gaskets Silicone rubber
Input/Output Tubing PE-90
Coverslips Top: 30 mm round, #1.5 Thickness
Bottom: 40 mm round, #1.5 Thickness
Microscope Stage Adapters* Uses Series 30
All major models:
Leica, Nikon, Zeiss, Olympus, Prior, Ludl, Burleigh
DOCUMENT DESCRIPTION
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure