Large Closed Bath Imaging Chambers (RC-21B, RC-21BR)

Large Closed Bath Imaging Chambers (RC-21B, RC-21BR)

Polycarbonate chambers providing a large viewing area and good access for microscope objectives.

  • Closed bath chamber design
  • Diamond fluidics
  • Ideal for fluorescent, calcium, and time-lapse imaging studies
  • Uses 22 mm square or 25 mm round coverslips
  • Large optical imaging area
  • Good access for upright or inverted microscopes

Item# Description U.S. List Price Quantity
64-0224 Large Bath Imaging Chamber, RC-21B, 22 mm Square
64-0225 Large bath Imaging Chamber, RC-21BR, 25 mm Round
64-0278 Series 20 Plain Platform, Model P-2
64-0285 Series 20 Heater Platform, Model PH-2
64-1561 PM-2 Chamber platform with magnetic clamps
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0704 CS-22S Coverslips for use with RC-21B Chamber, 0.15 mm (0.006 in), 22 x 22 mm, pkg of 100
64-0705 CS-25R Coverslips, 0.15 mm (0.006 in), 25 mm diameter, pkg of 100
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
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The RC-21B and RC-21BR chambers feature a closed bath, short working distances, and linear solution flow. Distance between the top and bottom coverslip is 2.5 mm and total bath volume is 358 µl.

At the bath output, a length of tubing is used as a back pressure control to insure smooth solution flow. An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe. This port is useful for injecting substances or for removing air bubbles from the sealed chamber.

Studies involving physiological bicarbonate buffers can be performed with these chambers with no gas loss at the chamber interface. The closed design allows the use of permeable supports. With laminar flow, minimal distortion of the sample occurs during perfusion.

RC-21B and RC-21BR chambers require a platform (P-2, PH-2, or PM-2) and stage adapter to complete the assembly for mounting onto a microscope.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22S and CS-25R, respectively, 100/pkg) and 10 ft of PE-160 polyethylene tubing.

Volume360 ul bath volume for 2.5 mm of solution
Material polycarbonate polycarbonate
Bath dimensions
(L x W x H)
17 x 13 x 2.5 mm 17 x 13 x 2.5 mm
Outer dimensions
(L x W x H)
47.5mm x 28.5mm x 2.5mm 47.5mm x 28.5mm x 2.5mm
Volume 358 μl 358 μl
Top coverglass 22 mm square 25 mm round
Bottom coverglass 22 mm square 25 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel 0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)

RC-21 drawing


  1. Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
    Kwinter D, Michael S
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  2. Monitoring the in vivo redox state of plant mitochondria: Effect of respiratory inhibitors, abiotic stress and assessment of recovery from oxidative challenge
    Schwarzländer M, Fricker MD, Sweetlove LJ
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  3. Influence of the Dissolution Rate on the Collapse and Shedding Behavior of Monostearin/Monopalmitin-rich Coated Microbubbles
    Shen Y, Powell RL, Longo ML
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  4. Confocal imaging of glutathione redox potential in living plant cells
    Schwarzländer M, Fricker MD, Müller C, Marty L, Brach T, Novak J, Sweetlove LJ, Hell R, Meyer AJ
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  5. Intracellular pH Homeostasis Plays a Role in the Tolerance of Debaryomyces hansenii and Candida zeylanoides to Acidified Nitrite
    Mortensen HD, Jacobsen T, Koch AG, Arneborg N
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  6. Characterizing molecular probes for diffusion measurements in the brain
    Kaur G, Hrabetova S, Guilfoyle DN, Nicholson C, Hrabe J
    J Neurosci Methods. 2008 Jun 30;171(2):218-25. Epub 2008 Mar 28
  7. Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
    Anggono V, Cousin MA, Robinson PJ
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  8. A Fluorescent Sensor for Imaging Reversible Redox Cycles in Living Cells
    Evan W. Miller, Shelly X. Bian, Christopher J. Chang
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  9. NHE1 inhibition by amiloride- and benzoylguanidine-type compounds. Inhibitor binding loci deduced from chimeras of NHE1 homologues with endogenous differences in inhibitor sensitivity
    Pedersen SF, King SA, Nygaard EB, Rigor RR, Cala PM
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  10. The Polypeptide Composition of Moving and Stationary Neurofilaments in Cultured Sympathetic Neurons
    Yan Y, Jensen K, Brown A
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  11. D-box is required for the degradation of human Shugoshin and chromosome alignment
    Fu G, Hua S, Ward T, Ding X, Yang Y, Guo Z, Yao X
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  12. Chemical inhibition of the Pho85 cyclin-dependent kinase reveals a role in the environmental stress response
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