Heka Data Acquisition

Heka Data Acquisition

With PATCHMASTER, FITMASTER and CHARTMASTER experimental design, performance, and analysis are more flexible allowing a high degree of automation and providing access to experimental protocols that are otherwise unattainable.

  • Operates on Windows and Macintosh platforms
  • Full software control of HEKA patch clamp amplifiers
  • Calibrates and tests the patch clamp amplifiers of the EPC 9 and EPC 10 Series
  • Features adaptive feedback control and global variables
  • Suitable for research and industry

Item# Description U.S. List Price Quantity
89-5035 LIH 8+8 Computer Interface (Acquisition Board), 90-250 VAC, 50/60 Hz, CE
89-5040 PATCHMASTER
89-5042 PATCHMASTER Upgrade
89-5041 PATCHMASTER Pro
89-5048 CHARTMASTER
89-5046 FITMASTER
89-5047 FITMASTER Pro
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patchmaster software

PATCHMASTER

Multi-channel data acquisition software

PATCHMASTER offers features meeting the highest standards of modern electrophysiology. This software, running on PC-based and Macintosh computers, harbors all the comfortable functions of PULSE, but offers a variety of novel procedures aimed to make electrophysiological research more versatile and efficient.

With PATCHMASTER, experimental design, performance, and analysis are more flexible, giving rise to a high degree of automation and providing access to experimental protocols that are unattainable with commercial software.

Major innovations of the PATCHMASTER program

More channels

PATCHMASTER provides up to 8(16) A/D input channels and 4(8) D/A stimulation channels that can be sampled at high speed. In addition, the number of channels is no longer limited to the number of available AD/DA channels.

Virtual traces

Acquired data traces can be processed online by mathematical functions to compile additional derived data traces.

Extended trigger possibilities

Up to 16 trigger outputs can be defined as a stimulation channel.

Individual timing for different channels

Channels can have completely independent timing and pulse patterns. This allows for easy setup of complex stimulation patterns for multiple channels.

Data compression

For all channels, individual data compression factors can be specified to reduce the amount of stored data.

Global parameters for sequence editing

For definition of a pulse generator sequence, global parameters can be used (e.g. to define the duration of a segment or the amplitude of a stimulation). If these parameters are used in multiple segments or sequences, all locations can be edited by changing a single global parameter.

Multiple Telegraphing Amplifiers

More than one telegraphing amplifier can be supported by using multiple lookup tables.

Extended Lock-In

Simultaneous capacitance measurements on multiple headstages of a patch clamp amplifier (EPC10 Double, Triple, and Quadro) and on-cell capacitance measurements at high sine wave frequencies are now possible.

Extended Online Analysis

An arbitrary number of online functions and methods can be defined and saved. Even complex calculations are now possible. Online analysis methods can be directly linked to acquisition sequences.

Two Online Analysis Windows

Up to 12 graphs can be displayed in two different online analysis windows. For example, one window can be used to display series derived analyses such as IVs, and the other for display of time lapse data.

Photometry feature

Multi-wavelength stimulation for multiple fluorescence excitation systems and analysis for multiple fluorescence signals is now supported.

Protocol Editor for automation

A complete experiment can be automated and standardized, including incorporation of feedback from online analysis or external devices.

PATCHMASTER- a program for patch clamp, 2-electrode voltage clamp experiments, and general data acquisition

Full support of HEKA's patch clamp amplifiers EPC7, EPC8, EPC9, and the EPC10. The novel program design of PATCHMASTER is perfectly suited for the operation of multi-channel stimulation as used for patch clamp amplifiers with multiple headstages (e.g. EPC9 Double/Triple, EPC10 Double/Triple/Quadro).

Additionally, PATCHMASTER can be run with any other patch clamp amplifier, electrophysiological current clamp or voltage clamp devices (e.g. two-electrode voltage clamps used for research on Xenopus oocytes), or a standalone data acquisition interface for general stimulation/data acquisition purposes.


 

PATCHMASTER Interface

 

Powerful software for acquisition and analysis of electrophysiological data

PATCHMASTER software

 


 

The virtual front panel of EPC10 patch clamp amplifier

PATCHMASTER software

 


 

Flexible Pulse Generator configures stimulation and acquisition

PATCHMASTER software

 



PATCHMASTER software

PATCHMASTER Protocol Editor

The protocol editor is a completely new feature of PATCHMASTER. With this editor, complex experimental procedures can be designed, stored, and executed. This tool greatly increases the versatility of PATCHMASTER and provides means for automatic experiment performance.

The principal idea of the protocol editor is to generate a list of events or tasks, which then are executed automatically. Various functions such as repeat loops, input queries, or conditional statements allow for the generation of complex interactive processes.

PATCHMASTER software

Online Analysis - Powerful online and offline data processing

An arbitrary number of analyses can be performed on newly acquired or replayed data. Directly analyzed data or derivative data, obtained by application of mathematical functions on the analysis results, are then displayed in a versatile manner in several graphs placed in two independent windows. This allows for separation of different data types, for example, current-voltage plots are shown separately from time-based data (e.g. chart recording).

Analysis templates can be predefined and stored. Thus, several analysis procedures are available such that various incoming data types can be analyzed without extra editing just by switching between analysis procedures. A direct link between Pulse Generator sequences and analysis procedures provides definition of data acquisition and analysis prior to the experiment

PATCHMASTER software

Implemented Lock In modes

In 1982, Neher and Marty introduced the lock-in amplifier into the patch clamp field for Cm measurements using a single sine wave frequency. For determining the appropriate phase setting, they used dithering or the compensation network while changing the phase for obtaining a maximum signal. This method is appropriate under stationary conditions for measurements of changes in Cm and we refer to this method as the "piecewise-linear" method.

Since the piecewise-linear method is prone to errors (Gillis in B.Sakmann & E.Neher Eds. Single Channel Recording 2nd Edition, Plenum Press), in 1988 Lindau and Neher introduced a method using the real and imaginary part of the admittance, plus the DC-conductance, to determine the absolute values of Cm, membrane conductance, and access resistance. We refer to this method as the "Sine+DC" method.

The best resolution of small changes in Cm are achieved in the cell-attached patch clamp configuration. Since a different equivalent circuit applies in this recording mode, a third method, referred to as the "on-cell" method, has been implemented.

Different modes of calibration

Digital control of the filter settings, gain and compensation networks that are featured with the EPC9 and EPC10 patch clamp amplifiers directly benefit the calculated calibration mode. Phase shifts introduced by the measuring system can be calculated and the corrected phase of the lock-in amplifier, which is dependent upon the recording conditions, can be set automatically.


A measured calibration method allows the phase and attenuation of the recording system to be determined by analysis of the admittance of a pure resistor at the amplifiers input.

In case other procedures for determination of the phase and attenuation of the measuring system are used, a manual calibration mode allows the phase and attenuation of the software lock-in to be directly set by the user.

Lowest noise recordings

In the Sine+DC mode, automatic Cslow compensation cancels the bulk of the membrane capacitance and thus permits the patch clamp amplifier to be operated in the high gain (low noise) range during lock-in measurements. In the on-cell mode, the signal to noise ratio can be increased by using a higher sine wave frequency (typically 20 kHz) to resolve small changes in Cm (< 100 aF), commonly seen in fusion of single vesicles.

Simultaneous measurements from multiple patch clamp amplifiers

In combination with EPC9 or EPC10 Multi-Patch amplifiers, simultaneous Cm measurements on multiple amplifiers are supported by our software lock-in.

Online equivalent circuit parameter calculation software lock-in provides online calculation of equivalent circuit parameters and offline recalculation. Customer specific calculations can be done online by using the virtual trace feature of the pulse generator in PATCHMASTER.

 



FITMASTER

Data Review, Analysis and Fitting

FITMASTER features analysis and fitting routines for electrophysiological data. Analysis can be performed on the levels of Sweeps/Traces and Series. Besides standard fit functions such as Polynomials, Exponentials, Gaussians, and Boltzmanns, tailored functions to fit e.g. whole-cell current traces according to Hodgkin&Huxley gating formalism, current-voltage relationships and dose-response curves allow publication-proof analysis of your data.

The experienced patch clamp investigator will be fascinated by the versatility of the program and logical structure of the functions. All of the features of PATCHMASTER's Online Analysis are also present in FITMASTER. In principle, FITMASTER extends these capabilities by adding more specific analysis features and the generation of an analysis tree. The way FITMASTER works is tightly linked to the tree structure of data generated by PATCHAMSTER up to the level of a Series. Hence, FITMASTER has equivalent hierarchical levels of analysis corresponding to the tree levels featured in the Replay data tree. The analysis is organized in a structured way, relating to Sweeps and Series. The lowest level is the analysis of a raw data trace, a Trace. The TraceFit dialog provides tools for selecting a section of the trace for analysis and specifications of the type of analysis to be performed. The values determined by this sweep analysis can be further processed in SeriesFit. But in contrast to PULSEFIT, FITMASTER allows pooling of data on the level of the Series by introducing the new waves buffer concept. Several results of the TraceFit can be compiled in the waves buffer and then displayed in the SeriesFit graph. Several fit functions can be used to describe this ensemble of data points.

FITMASTER now features Action Potential analysis ...

... and Single Channel detection.

 



CHARTMASTER

Data Acquisition and Control Software

The CHARTMASTER software was designed with the main objective to streamline data collection, presentation and analysis during and after an experiment.

CHARTMASTER provides all of the necessary software tools that make experimental design and analysis more flexible.

With CHARTMASTER's high degree of automation, experimental protocols are now possible that were unattainable with other commercial software products.

CHARTMASTER's versatile design allows it to be used for any general purpose data acquisition need. In addition, CHARTMASTER can be used in conjunction with PATCHMASTER to provide even greater capabilities for elaborate electrophysiological recordings.

Pulse Generator


The heart of the CHARTMASTER software is the Pulse Generator. The Pulse Generator defines all of the parameters for data acquisition, waveform generation and external device control. A Pulse Generator file is comprised of any number of predefined sequences.

Protocol Editor

The CHARTMASTER protocol editor is a powerful feature in which complex experimental procedures can be designed, stored, and executed. This tool greatly increases the versatility of CHARTMASTER that will be appreciated by researchers asking for complex, precisely timed experimental protocols.

The principal idea of the protocol editor is to generate a list of events or tasks which can be executed automatically. Various functions such as REPEAT Loops, input queries, or conditional statements allow the generation of complex interactive processes. In addition, the high degree of automation possibilities increases efficiency, minimizes experimental errors and is thus highly suited for both industrial and research applications.

Online Analysis

The CHARTMASTER software provides an arbitrary number of analyses that can be performed on newly acquired or stored data. Directly analyzed or derivative data obtained by application of mathematical functions on the analysis results can be displayed as several graphs placed in two independent analysis windows.

This allows separation of different data types, for example, current-voltage plots can be shown separately from time lapse data (e.g. chart recording).

Input/Output Control


The I/O Control window allows direct access to the hardware interface. The status of digital and analog input channels is monitored. Digital and analog output signals can be set. In addition, defined input parameters are also displayed.

Analysis templates can be predefined and stored. Thus, several analysis procedures can easily be switched between and applied to incoming data types without extra editing. A direct link between Pulse Generator sequences and analysis procedures provides definition of data acquisition and analysis prior to the experiment.

 


 

LIH 8+8

16-bit Multi-Channel Data Acquisition System

LIH 8+8

The LIH 8+8 is a high resolution, low-noise scientific data acquisition system that utilizes the latest USB 2.0 and high speed processing technologies. The LIH 8+8 provides expandability and versatility that will satisfy both current and future needs. With its USB 2.0 interface, the LIH 8+8 can easily be connected to a laptop computer without the need for a peripheral PCI card. The analog input and output channels are isolated from the digital lines that communicate with the computer. Each analog channel has it's own separate ground patch and the digital section has a completely different ground. The result is complete isolation of the acquisition side from the computer side with full 16 bit capability and low noise.

Expandability

The LIH 8+8 system is comprised of a USB 2.0 computer interface and one or multiple analog rack units interconnected by CAT5 cables. The USB 2.0 provides superb performance, while the CAT5 cables allow multiple racks to be synchronized during data acquisition. A single external trigger is capable of starting multiple racks simultaneously.

The number of racks supported by one computer is only limited by the number of USB connectors available in that computer. Multiple racks installed in the same computer or in separate computers can be synchronised.

Input and Output Channels

The LIH 8+8 provides eight analog differential inputs, four analog differential outputs via BNC connectors at the front panel. Sixteen digital inputs and sixteen digital outputs are provided on one connector at the rear panel. In parallel, 4 digital outputs and 4 digital inputs are provided via BNC connectors on the front panel. All channels are sampled synchronously.

Sound Generator

The LIH 8+8 has a sound generator built-in. A sound connector at the rear panel allows connection of an active speaker or a headset.

Acquisition Mode

The LIH 8+8 features co-phasic acquisition of the two most relevant signals; ex. the current and voltage trace of the amplifier. The acquisition board samples these two AD-converters in parallel with no time delay. In the schematic below the two AD channels exhibiting co-phasic acquisition are AD-0 and AD-1.

The eight analog differential input channels are separated into two banks of four. One bank is comprised of AD channels 0,2,4 and 6 and the other bank is comprised of AD channels 1,3,5 and 7. Each bank is multiplexed into one 16-bit 200 kHz A/D converter. Both A/D converters sample simultaneously and synchronously at the maximum conversion rate resulting in a total throughput of 400 kHz. This unique arrangement allows pairs of channels to be digitized without phase-shift (ex; AD-0 & AD-1). In other words, there is no time delay between two DA-channels firing and the time of acquisition of two co-phasic AD-channels. If the bandwidth of the experiment calls for lower sampling rates, the interface adjusts the rate accordingly.

The LIH 8+8 can be controlled with PATCHMASTER or CHARTMASTER software, on either a Windows or Mac platform. TIDA software can also be used to control the LIH 8+8 on an older Windows (2000/XP) platform. In situations where the LIH 8+8 is being integrated in a self-written data acquisition software on Windows, HEKA provides an EPC DLL (dynamic link library).

Specifications

LIH 8+8 Specifications

Analog Inputs

  • Eight 16-bit analog inputs
  • Two A/D converters, each multiplexed into 4 inputs
  • 400 kHz aggregate, max. 200 kHz per A/D converter
  • Input type: differential
  • Input Range: ±10.24 V
  • Differential non-linearity: ±0.002% of FSR
  • Drift: ±2 ppm/°C
  • Input impedance: 1 MΩ
  • Signal to noise ratio: 86 dB, DC-160 kHz, <1mV PP

Digital Inputs

  • Sixteen rear digital inputs
  • 4 of the 16 digital inputs can be accessed on the front panel via BNC
  • 3.3 and 5V logic compatible

Trigger Inputs

  • One isolated hardware trigger input (on either positive or negative edge transition)
  • 3.3 and 5V logic compatible
  • Single BNC on rear panel

Sound output

  • One 3.5 mm output receptacle on rear panel
  • Frequency range of 0.2-4.0 kHz

Additional Connectors

  • Digital I/O connector for EPC8 or TIB 14S
  • Two CAT5 connectors for slave/master operation of multiple LIH 8+8

Dimensions

  • 47.5 x 4.4 x 31.1 cm
  • Weight: 3.6 kg
  • Mounts in 19" rack, mounting hardware included
  • Operates on 90-250 VAC, 50/60 Hz

Cable

  • USB 2.0 cable

Host Interface

  • USB 2.0

PATCHMASTER Specifications

Amplifier Control

  • All 'clamp' amplifiers are supported. Amplifier windows for all EPC9 and EPC10 amplifiers and telegraphing amplifiers are available.
  • Automatic test and calibration routines for all HEKA EPC9 and EPC10 amplifiers are provided.

Data Acquisition

  • Up to 16 input channels
  • Pulsed and continuous acquisition mode
  • Automatic data compression (different sample rates for different channels)
  • Virtual trace for mathematical online processing of acquired channels
  • For each acquisition channel a break criteria can be defined. An Automatic Break will stop the acquisition whenever one of break criteria becomes true.
  • Variable data format for storage (INTEGER, REAL, LONGREAL)

Leak Subtraction

  • Leak pulses (p/n correction) supported for all output channels (important for multi-headstage clamps)
  • Various leak pulse storage modes: none, average, all (important for offline leak correction)

Stimulation

  • Up to 4(8) stimulation channels
  • Stimulation with an arbitrary number of pulse segments
  • Segment types: constant, ramp, sine, square, non-stored
  • Various increment modes for segment amplitude and duration
  • Segment parameters assignable by global variables (pgf-parameters)

Digital Oscilloscope

  • Display of up to 20 different traces
  • Individual display scaling and visual appearance for all traces
  • Individual digital filter settings for all traces
  • Various different labeling modes (e.g. Grids+Labels, Grids+Values, Labels only)
  • Zoom
  • Dimmed overlay
  • Absolute and relative measurements with mouse click
  • Read individual data points with scan function

Online Analysis

  • Arbitrary number of user defined online methods
  • More than 40 predefined analysis functions
  • Mathematical standard operations allow setup of user defined analysis functions
  • Trace operation functions
  • Two online windows with up to 12 graphs for graphical representation of analysis results.
  • Link of analysis methods to different acquisition sequences

Trace Buffer

  • Four independent trace buffers are available for basic arithmetic operations on the level of a trace (add, subtract, accumulate, de-accumulate)
  • Buffer traces can be shown as a reference trace in the oscilloscope window.

I/O Control

  • Direct access to set digital outputs, analog outputs, input parameters and to send serial commands.
  • Monitoring of digital inputs, analog inputs and input parameter values.

Standardization and Automation

  • Macros: A series of user defined actions can be recorded and stored as a macro.
  • Protocol Editor: The Protocol Editor allows standardization and automation of complete experimental procedures. Within the procedure, the system can get feedback from external inputs, amplifier controls, online analysis results or user inputs and experimental parameters can be adjusted. A protocol can be started/called from another protocol.
  • Batch Control: The complete PATCHMASTER acquisition system can be controlled from another application. The user can write their own application with a custom tailored user interface but still benefit from the advanced features of the HEKA system.

Data Integrity

  • Acquired data are organized and stored in a data tree. Multiple data packages are usually stored in one data file and organized in levels of Groups/Experiments/Series/Sweeps and Traces to allow easy review, selection and analysis of all data in that data file.
  • Due to the complete software control, a complete set of parameters describing the state of the amplifier and other recording conditions is stored with the data. This allows detailed reconstruction of the experiment for exact analysis at later times.

Data Export

  • Export and printout of raw data traces or online analysis results
  • Supported formats: ASCII, IGOR PRO, MathLab and WMF
  • High level layout features of IGOR PRO supported.

Other Modules

Software lock-in:

    PATCHMASTER features a software lock-in amplifier for time resolved measurements of membrane capacitance.
  • Sine+DC, Piecewise-linear and On-Cell capacitance measurements modes
  • Calculated calibration mode allows automatic correction for phase lag introduced by the EPC9 or EPC10 patch clamp system
  • Measured and Manual calibration modes for highest accuracy in challenging recording modes
  • Lock In measurements on multiple amplifiers are supported (EPC9 and EPC10 multi patch amplifiers required)

Photometry Extension:

  • Support of fluorescence excitation light sources TILL Polychrome, PTI DeltaRAM, SUTTER DG4/DG5, Lambda-10
  • Multi-excitation and multi-emission protocols
  • Simultaneous high-speed fluorescence and patch clamp measurements

Compatibility

  • Runs on Windows 98, NT 4.0, 2000, XP, 7, 8, 10. Requires USB port.
  • Runs on Mac OS 9 and Mac OS X. Requires a USB port.
  • PATCHMASTER can read data recorded with PULSE or generated with PULSETOOLS or PULSESIM.

Hardware Requirements

  • Data acquisition: EPC9 or EPC10 type patch clamp amplifier or ITC-16, ITC-18, LIH 1600, or LIH 8+8 data acquisition interface in combination with any other clamp amplifier.
  • Computer: see data sheet "Recommended Configurations".

FITMASTER Specifications

TraceFit

  • Even complex mathematical expressions can be analyzed with the Online Analyis and then processed with SeriesFit in FITMASTER. Online methods created with PATCHMASTER can be used in FITMASTER.
  • TraceFit supports the analysis and the storage of results from multiple functions for a given series. A set of results for a given series, for example, can consist of parameters from different analysis and fit functions or form results retrieved from different segments of a trace.
  • When executing data fits in TraceFit, data sections of interest can be zoomed automatically to provide an optimal view necessary to decide upon the quality of the fit.
  • Performs multiple analyses on a single data trace. Useful to analyze responses to tetanus stimulation or other cyclic high speed stimulation.

SeriesFit

  • Results from single or multiple analyses can be compiled in a work buffer before being processed by SeriesFit. This way, data originating from different series can be merged (appended) for later analysis.
  • In addition to appending SweepFit results to the analysis work buffer, FITMASTER also allows data with identical X-references to be processed. One can form e.g. the average of multiple results. Such averaged data are then shown with or without error bars and a fit function can be fit to the mean data.
  • Math functions allows some basic mathematical functions average, “+”, “–“, “*”, “/” to be performed between data sets.
  • In addition, the content of the work buffer can be processed with the functions “invers”, “square”, “sqrt”, “ln”, “exp”, “+ const”, “- const”, “* const”, “/ const”.
  • The “mean” and “standard deviation” of the entire work buffer content are shown at the bottom of the columns at any time.
  • Data in the work buffer can be sorted according to their X- or Y- values.
  • Data points from the work buffer can be omitted from analysis. Results from non-converged fits in TraceFit are automatically marked as skipped.
  • The SeriesFit graph can be scaled and modified in many ways such as changing the colors, tics, scaling of the axes etc. In addition, the axis range can now be defined as “fixed” or “auto” for minimum and maximum as well as X- and Y-axis separately. The scale range in the “auto” mode can be based either on all data points or on the active, i.e. non-skipped, data points only. The data in the graph can be offset or normalized easily.
  • By clicking of individual data points in the SeriesFit graph, the number and value is shown in the notebook. Also toggling of the skip status can be performed by a mouse click.
  • Multiple background traces can be stored and overlaid to the SeriesFit graph.

Export

  • Data and fits of the TraceFit and SeriesFit can be exported into ASCII and IgorPro format. The export mode can be configured to customize output.
  • Fit parameters in TraceFit as well as in SeriesFit can be sent to the clipboard with a single mouse click and transferred to other programs easily.

Compatibility

  • Runs on Windows 98 / NT4.0 / 2000 / XP (requires a parallel printer port on Windows computers).
  • Runs on Mac OS 9 and Mac OS X (requires a USB port on Macintosh computers).
  • FITMASTER can read sweep data recorded with PATCHMASTER and PULSE or generated with PULSETOOLS or PULSESIM.

CHARTMASTER Specifications

Data Acquisition

  • Up to sixteen input channels
  • Episodic and continuous acquisition mode
  • Automatic data compression (different sample rates for different channels)
  • Virtual trace for mathematical online processing of acquired channels
  • A break condition which can terminate all ongoing acquisition can be defined for each acquisition channel
  • Selectable data format for storage (INTEGER, REAL, LONGREAL)

Waveform Generation

  • Up to sixteen waveform output channels
  • An output waveform can be generated with any arbitrary number of pulse segments
  • Output waveform can be created from external template file
  • Segment types: constant, ramp, sine, square, non-stored or continuous
  • Various increment modes for segment amplitude and duration
  • Segment parameters can be assigned by global variables (pgf-parameters)

Digital Oscilloscope

  • Display of up to twenty different traces
  • Individual display scaling, offset adjustment and visual appearance for all traces
  • Individual digital filter settings for all traces
  • Various labelling modes (e.g. Grids+Labels, Grids+Values, Labels only)
  • Zoom
  • Dimmed trace overlay
  • Absolute and relative measurements with mouse click
  • Individual data points can be read with scan function

Online Analysis

  • Arbitrary number of user defined online methods
  • Over forty predefined analysis functions
  • Standard mathematical operations allow creation of user defined analysis functions
  • Trace operation functions
  • Two online windows with up to twelve graphs for graphical representation of analysis results
  • Link of analysis methods to different acquisition sequences

Trace Buffers

  • Four independent trace buffers for performing basic arithmetic operations on a data trace (i.e. add, subtract, accumulate, deaccumulate)
  • Trace buffers can be displayed as a reference trace in the oscilloscope window

I/O Control

  • Direct control of digital and analog outputs
  • Serial command protocols for external device control
  • Active monitoring of digital and analog inputs as well as input parameter values
  • Direct access to global vaiables

Standardization and Automation

  • Macros: A series of user defined actions can be recorded and stored as a macro
  • Protocol Editor: The Protocol Editor allows complete experimental procedures to be easily created. Within a procedure, feedback from external inputs, amplifier controls, online analysis results or user inputs and experimental parameters can be adjusted. A protocol can be started / called from another protocol.
  • Batch Control: The CHARTMASTER acquisition program can be controlled from another software application. This allows custom applications, with their own user interface, to take advantage of all of the data acquisition features of CHARTMASTER while adding or subtracting features specific to their requirements.

Data Integrity

  • Acquired data are organized and stored in a data tree format. Multiple data acquisition runs can be stored in a single data file organized in levels of Groups / Experiments / Series / Sweeps and Traces. This allows easy review, selection and analysis of all the acquired data stored in the data file.
  • CHARTMASTER’s extensive software control provides a thorough set of parameters, describing the state of the recording conditions, to be stored along with the data. This allows detailed reconstruction of the experiment for exact analysis when reviewing the acquired data.

Data Export

  • Export and printout of raw data traces or online analysis results
  • Supported formats: ASCII, IGOR PRO (including high level layout features), MATLAB and WMF

Built-in Modules

  • Software Lock-In: CHARTMASTER features a software Lock-In amplifier for time-resolved measurements of admittance
  • Photometry Extension: Allows simultaneous high-speed fluorescence and data acquisition with either a TILL Photonics Polychrome, PTI DeltaRAM, Sutter Instrument Lambda 10, DG4, or DG5 light source with multi-excitation and multi-emission protocols
  • Solution Data Base: Allows definition and organisation of multiple solutions. Selected solutions can be linked to the acquired data

Compatibility

  • Compatible with Windows 2000, XP, Vista or Mac OS X (10.4 and above with either a PPC or Intel processor)
  • CHARTMASTER can read data files created by PATCHMASTER, PULSE, PULSETOOLS or PULSESIM

Supported Data Acquisition Interfaces

  • HEKA LIH 1600
  • InstruTECH LIH 8+8
  • InstruTECH ITC-16 and ITC-18

Minimum Computer Requirements

  • Mac OS X: Macintosh G5 or faster processor, Mac OS X (10.4 and above), 256 MB RAM, available USB port, CD-ROM or Internet access
  • Windows: Pentium 4 or faster processor, Windows 2000, XP or Vista, 256 to 512 MB RAM, available USB 2.0 port, CD-ROM or Internet access