Haas Top (BSC-HT)

Haas Top (BSC-HT)

The BSC-HT Haas Top is an interface-type chamber utilizing semi-submersion principles for the study of brain/tissue slices.

  • Maintains a stable intracellular membrane potential of more than 60 mV
  • Facilitates action potentials with overshoots close to 100 mV in amplitude, duration of less than 2 msec at 32°C, when used with hippocampal pyramidal and granular cells
  • Maintenance of the above potentials is possible for more than 1 hour undisturbed by changes in perfusion fluids
  • Preparation slices remain viable for up to 12 hours
  • Using flow rates of 1 ml/min, will completely exchange the chamber fluid in less than 1 minute

Item# Description U.S. List Price Quantity
64-1525 (BSC-BUW) BSC-BU base unit compatible with Warner temperature controllers, includes connecting cable
65-0074 Model BSC-ZT (Zbicz Top) for Tissue Slice Chamber
65-0075 Model BSC-HT (Haas Top) for Tissue Slice Chamber
65-0076 Model BSC-PC Prechamber (for holding slices) Tissue Slice Chamber
64-0100 (TC-324B) Heater Controller, single channel, 110-120 VAC, 60Hz
64-0101 (TC-344B) Heater Controller, dual channel, 110-120 VAC, 60Hz
65-0073 Model BSC-BU (Base Unit) Tissue Slice Chamber
65-0045 Model TC-202A Bipolar Temperature Controller for use with PDMI-2, PSMI, CSMI, LU-CB-1, LU-CPC-CEH, and BSC-BU.
65-0078 Model BSC-AIR Aeration Element Replacement, Set of 2
65-0081 Model BSC-TUBE Base Unit (Model BSC-BU) Tubing Set Replacement, Tygon .031 x .093 (ID x OD)
65-0079 Model BSC-ORING Base Unit (Model BSC-BU) Replacement Gasket Set
65-0082 Model BSC-G10 Haas Top (Model BSC-HT) Tissue Insert Replacement, Set of 2
65-0083 Model BSC-HTC Haas Top (Model BSC-HT) Plastic Cover Replacement
65-0084 Model BSC-HT-AG Haas Top (Model BSC-HT) Ground Wire Replacement
65-0086 Model BSC-ZTC Zbicz Top (Model BSC-ZT) Plastic Insert Replacement
65-0087 Model BSC-PC-VAL Prechamber (Model BSC-PC) Valve Replacement
65-0608 Insert for Model BSC-PC Prechamber Tissue Slice Chamber
65-0090 Model BSC-MESH Haas Top (Model BSC-HT) Zbicz Top (Model BSC-ZT) Replacement Mesh 300 µm grid, 5 x 5 in, pkg. of 4
65-0088 Model BSC-ACC-KIT Base Unit, Haas & Zbicz Tops Accessory Kit (Replacement Screws, Fittings and Mesh)
64-1697 (BSC-HEW) Replacement heating wire for BSC-BUW
64-1591 (BSC-CABLE-W) BSC=BU connecting cable for Warner temperature controllers
65-0080 Model BSC-HE Base Unit Heating Element Replacement
65-0091 Model BSC-CABLE Base Unit (Model BSC-BU)/Temperature Controller (Model TC-102) Replacement Power Cable
65-0104 Output Cable, 6-Pin to Banana for 65-0045 Model TC-202A or 65-0073 Model BSC-BU
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The Haas Top is an interface-type chamber utilizing semi-submersion principles for the study of brain/tissue slices.

  • Maintains a stable intracellular membrane potential of more than 60 mV
  • Facilitates action potentials with overshoots close to 100 mV in amplitude, duration of less than 2 msec at 32°C, when used with hippocampal pyramidal and granular cells
  • Maintenance of the above potentials is possible for more than 1 hour undisturbed by changes in perfusion fluids
  • Preparation slices remain viable for up to 12 hours
  • Using flow rates of 1 ml/min, will completely exchange the chamber fluid in less than 1 minute

The Haas Top (BSC-HT) provides for rapid exchange of superfusing fluids, and excellent stability for intracellular recording. Normally used in conjunction with the BSC-BUW or BSC-BU base units and a temperature controller. Slices rest on nylon mesh below which a thin sheet of the perfusate of interest flows. The bottom side of the slice is wet as capillary action saturates the mesh. Slices are oxygenated by a warmed and humidified mixture of 95/5% O2/CO2 which flows across the top surface of the slice. The BSC-HT has been designed so that the effects of two drugs can be studied simultaneously. One drug flows down the left portion of the chamber and the second down the right.

Specifications
Dimensions
(L x W x H)
111.13 x 85.41 x 23.80 mm
(4.375 x 3.365 x 0.937 in)
Weight 130.41 g (4.60 oz)