Fast Exchange Diamond Bath Chambers (RC-24 and RC-24E)

Fast Exchange Diamond Bath Chambers (RC-24 and RC-24E)

The RC-24 and RC-24E chambers allow for very fast solution exchange for a variety of applications. These recording chambers feature the smallest bath volumes of Warner's open style chambers.

  • Designed for physiological measurements of cell cultured coverslips
  • Applications include patch clamp, imaging, and intracellular/extracellular recordings
  • Diamond shaped bath provides laminar solution flow
  • Both perfusion and aspiration ports on same side of chamber
  • Very small bath volume facilitates very fast solution exchange

Item# Description U.S. List Price Quantity
64-1431 Straight Suction Tube, Series 20 Classic Design for use with RC-24, RC-24E, and RC-28 chambers
64-0274 Heater Elements (2) for PH (Heater) Platforms with Mounting Pads and Screws, RH-2
64-0107 TA-29 Replacement Cable with Bead Thermistor
64-0106 Model CC-28 Cable Assemblies with Connector for TC-324B/344B
64-0704 CS-22S Coverslips for use with RC-21B Chamber, 0.15 mm (0.006 in), 22 x 22 mm, pkg of 100
64-0707 CS-22/40 Coverslips, use with RC-22, RC22C, RC-24, RC-24E, RC-24N, RC-26, RC-26G, RC-26GLP, RC-30, and RC-50, pkg of 50
64-0756 (PE-160/100) Polyethylene tubing, PE-160, 100 ft
64-0755 PE-160/10 Polyethylene Tubing PE-160, 10 ft.
64-1920 (SA-20PS1) Adapter Ring for Pecon Heating Insert P
64-1506 (RPC-1) Replacement platform clamps and screws for P-1, P-3, P-4, P-5, and P-6 platforms, 2 pack
64-1526 PM-1 Chamber platform with magnetic clamps
64-0284 Series 20 Heater Platform, Model PH-1
64-0277 Series 20 Plain Platform, Model P-1
64-0231 Fast Exchange RC-24E Recording Chamber
64-0230 Fast Exchange RC-24 Recording Chamber
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The bath volume in these models is among the smallest of the open style chambers. This enables very fast solution exchange for a variety of applications, including patch clamp and electrophysiology studies. The small bath volumes are especially useful when using drugs in small quantities.

The RC-24 and RC-24E have a an unusual characteristic in that both the perfusion inlet and aspiration outlet is on the same side of the chamber. This allows the associated perfusion lines to be conveniently placed on only one side and facilitates electrode placement.

An agar bridge well for reference electrodes is provided, as is an aspiration well. The aspiration well is equipped with a stainless steel aspiration tube together with a clamp. The clamp insures that the adjustable suction tube will not move once the height is set by the user.

The floor of these chambers can be formed from either a 22 mm square coverslip or from a 22 mm x 40 mm rectangular coverslip. Each chamber requires a platform (P-1, PH-1, or PM-1) and a stage adapter to complete the assembly for mounting on a microscope.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22x40 mm, 50/pkg) and 10 ft of PE-160 tubing.

 RC-24 RC-24E
Material Polycarbonate Polycarbonate
Bath dimension
(L x W x H)
15 x 5 x 3.8 mm 15 x 6 x 3.8 mm
Volume, by depth 40 μl/mm 50 μl/mm
Bottom coverglass 22 x 40 mm 22 x 40 mm
Input tubing (ID x OD) 1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)

 RC-24 Drawing

  1. The Effect of Thermal Acclimation on Action Potentials and Sarcolemmal K+ Channels from Pacific Bluefin Tuna Cardiomyocytes
    Galli GL, Lipnick MS, Block BA
    Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R502-9. Epub 2009 Jun 10
  2. Analogs of MK-499 are differentially affected by a mutation in the S6 domain of the hERG K+ channel
    Karczewski J, Wang J, Kane SA, Kiss L, Koblan KS, Culberson JC, Spencer RH
    Biochem Pharmacol. 2009 May 15;77(10):1602-11. Epub 2009 Feb 27
  3. Pharmacological properties of a pore induced by raising intracellular Ca2+
    Faria RX, Reis RA, Casabulho CM, Alberto AV, de Farias FP, Henriques-Pons A, Alves LA
    Am J Physiol Cell Physiol. 2009 Jul;297(1):C28-42. Epub 2009 Mar 25
  4. D1 dopaminergic control of G protein–dependent inward rectifier K+ (GIRK)–like channel current in pyramidal neurons of the medial prefrontal cortex
    Witkowski G, Szulczyk B, Rola R, Szulczyk P
    Neuroscience. 2008 Jul 31;155(1):53-63. Epub 2008 Jun 19
  5. Modulation of Ca2+ channel current by mu opioid receptors in prefrontal cortex pyramidal neurons in rats
    Rola R, Jarkiewicz M, Szulczyk P
    Acta Neurobiol Exp (Wars). 2008;68(1):10-25
  6. High [Na+]i in cardiomyocytes from rainbow trout
    Birkedal R, Shiels HA
    Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R861-6. Epub 2007 May 16
  7. Decreased Neuronal Death in Na+/H+ Exchanger Isoform 1-Null Mice after In Vitro and In Vivo Ischemia
    Jing Luo, Hai Chen, Douglas B. Kintner, Gary E. Shull, and Dandan Sun
    The Journal of Neuroscience, December 7, 2005, 25(49):11256-11268
    Kim P. Larsson, Hanna M. Peltonen, Genevieve Bart, Lauri M. Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P. Kukkonen, and Karl E. O. Åkerman,
    J. Biol. Chem., Vol. 280, Issue 3, 1771-1781, January 21, 2005
  9. Stimulation of astrocyte Na+/H+ exchange activity in response to in vitro ischemia depends in part on activation of ERK1/2
    Douglas B. Kintner, Andy Look, Gary E. Shull, and Dandan Sun
    Am J Physiol Cell Physiol 289: C934-C945, 2005
  10. The contribution of ionic currents to changes in refractoriness of human atrial myocytes associated with chronic atrial fibrillation
    Workman AJ, Kane KA, Rankin AC
    Cardiovasc Res. 2001 Nov;52(2):226-35
Integrated Components Brochure_06_7_11.pdfIntegrated Components for Live Cell Imaging Brochure
RC-24 24E (080724).pdfRC-24 and RC-24E Chamber Instructions
perfusion_strategies.pdfPerfusion Strategies (Leinders-Zufall)