Fast Exchange Diamond Bath Chambers (RC-24 and RC-24E)
The RC-24 and RC-24E chambers allow for very fast solution exchange for a variety of applications. These recording chambers feature the smallest bath volumes of Warner's open style chambers.
- Designed for physiological measurements of cell cultured coverslips
- Applications include patch clamp, imaging, and intracellular/extracellular recordings
- Diamond shaped bath provides laminar solution flow
- Both perfusion and aspiration ports on same side of chamber
- Very small bath volume facilitates very fast solution exchange
The bath volume in these models is among the smallest of the open style chambers. This enables very fast solution exchange for a variety of applications, including patch clamp and electrophysiology studies. The small bath volumes are especially useful when using drugs in small quantities.
The RC-24 and RC-24E have a an unusual characteristic in that both the perfusion inlet and aspiration outlet is on the same side of the chamber. This allows the associated perfusion lines to be conveniently placed on only one side and facilitates electrode placement.
An agar bridge well for reference electrodes is provided, as is an aspiration well. The aspiration well is equipped with a stainless steel aspiration tube together with a clamp. The clamp insures that the adjustable suction tube will not move once the height is set by the user.
The floor of these chambers can be formed from either a 22 mm square coverslip or from a 22 mm x 40 mm rectangular coverslip. Each chamber requires a platform (PM-1, PH-1, or P-1) and stage adapter to complete the assembly for mounting on a microscope.
Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22x40 mm, 50/pkg) and 10 ft of PE-160 tubing.
(L x W x H)
|15 x 5 x 3.8 mm||15 x 6 x 3.8 mm|
|Volume, by depth||40 μl/mm||50 μl/mm|
|Bottom coverglass||22 x 40 mm||22 x 40 mm|
|Input tubing (ID x OD)||1.14 x 1.57 mm (PE-160)||1.14 x 1.57 mm (PE-160)|
|Aspirator coupling||1.67 mm (OD)||1.67 mm (OD)|