Excised Patch Chamber with Two Slotted Open Baths (RC-28)
The dual slotted open bath construction of the excise patch chamber permits exchange of solution surrounding a membrane patch without contamination of the associated cell population.
- Designed for excised patch studies
- Open chamber design
- Dual slotted baths in two sizes
- Baths separated by a controllable dam
The RC-28 is a special chamber containing two bath compartments separated by an adjustable dam. The dam functions both as a gate and barrier between the baths. The composition and flow rate of each bath can be independently controlled, and each bath has a separate stainless steel suction tube for perfusion.
The dual slotted, open bath design allows for the exchange of solution surrounding an isolated membrane patch without contaminating or modifying the rest of the population. Raising the solution level in the dam area joins the two baths, while lowering the solution level isolates the two baths. After patching onto a cell in the large bath compartment, the dam is flooded, enabling secure transfer of the electrode attached cell to the smaller compartment. The dam is then drained isolating the two baths.
For the larger chamber, the bath area is 21 x 9.5 mm and the working volume is 243 ul per 1.0 mm of solution height. For the smaller chamber, the bath area is 28 x 3.8 mm and the volume is 111 ul.
Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.
The RC-28 is supplied with a pack of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, 50/pkg) and 10 ft of PE-160 polyethylene tubing.
|Dimensions, small bath d
(L x W x H)
|20.0 x 3.8 x 4.5 mm|
|Dimensions, large bath d
(L x W x H)
|28.0 x 9.5 x 4.5 mm|
|Volume, small bath, by depth||111 µl|
|Volume, large bath, by depth||243 µl|
|Specimen area, small bath (L x W)||28 x 3.8 mm|
|Specimen area, large bath (L x W)||21 x 9.5 mm|
|Bottom coverglass||24 x 50 mm|
(ID x OD)
|1.14 x 1.57 mm (PE-160)|
|Aspirator coupling||1.67 mm (OD)|